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Open AccessArticle

Ets Family Transcription Factor Fli-1 Promotes Leukocyte Recruitment and Production of IL-17A in the MRL/Lpr Mouse Model of Lupus Nephritis

1
Department of Rheumatology, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan
2
Division of Rheumatology and Immunology, Medical University of South Carolina, Charleston, SC 29425, USA
*
Authors to whom correspondence should be addressed.
Cells 2020, 9(3), 714; https://doi.org/10.3390/cells9030714
Received: 15 February 2020 / Revised: 8 March 2020 / Accepted: 13 March 2020 / Published: 14 March 2020
(This article belongs to the Special Issue Molecular and Cellular Basis of Autoimmune Diseases)
The transcription factor Friend leukemia integration 1 (Fli-1) regulates the expression of numerous cytokines and chemokines and alters the progression of lupus nephritis in humans and in the MRL/MpJ-Faslpr (MRL/lpr) mouse model. Th17-mediated immune responses are notably important as they promote ongoing inflammation. The purpose of this study is to determine the impact of Fli-1 on expression of interleukin-17A (IL-17A) and the infiltration of immune cells into the kidney. IL-17A concentrations were measured by ELISA in sera collected from MRL/lpr Fli-1-heterozygotes (Fli-1+/−) and MRL/lpr Fli-1+/+ control littermates. Expression of IL-17A and related proinflammatory mediators was measured by real-time polymerase chain reaction (RT-PCR). Immunofluorescence staining was performed on renal tissue from MRL/lpr Fli-1+/ and control littermates using anti-CD3, anti-CD4, and anti-IL-17A antibodies to detect Th17 cells and anti-CCL20 and anti-CD11b antibodies to identify CCL20+ monocytes. The expression of IL-17A in renal tissue was significantly reduced; this was accompanied by decreases in expression of IL-6, signal transducer and activator of transcription 3 (STAT3), and IL-1β. Likewise, we detected fewer CD3+IL-17+ and CD4+IL-17+ cells in renal tissue of MLR/lpr Fli-1+/ mice and significantly fewer CCL20+CD11b+ monocytes. In conclusion, partial deletion of Fli-1 has a profound impact on IL-17A expression and on renal histopathology in the MRL/lpr mouse. View Full-Text
Keywords: Fli-1; MRL/lpr mouse; lupus nephritis; IL-17A; interstitial inflammation; CCL20; chemokines Fli-1; MRL/lpr mouse; lupus nephritis; IL-17A; interstitial inflammation; CCL20; chemokines
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Sato, S.; Zhang, X.K.; Temmoku, J.; Fujita, Y.; Matsuoka, N.; Yashiro-Furuya, M.; Asano, T.; Kobayashi, H.; Watanabe, H.; Migita, K. Ets Family Transcription Factor Fli-1 Promotes Leukocyte Recruitment and Production of IL-17A in the MRL/Lpr Mouse Model of Lupus Nephritis. Cells 2020, 9, 714.

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