Next Article in Journal
New Insights into X-Chromosome Reactivation during Reprogramming to Pluripotency
Next Article in Special Issue
Linking the Endocannabinoidome with Specific Metabolic Parameters in an Overweight and Insulin-Resistant Population: From Multivariate Exploratory Analysis to Univariate Analysis and Construction of Predictive Models
Previous Article in Journal
Calcium Increase and Substance P Release Induced by the Neurotoxin Brevetoxin-1 in Sensory Neurons: Involvement of PAR2 Activation through Both Cathepsin S and Canonical Signaling
Previous Article in Special Issue
Role of 2-Arachidonoyl-Glycerol and CB1 Receptors in Orexin-A-Mediated Prevention of Oxygen–Glucose Deprivation-Induced Neuronal Injury
 
 
Article

Mgll Knockout Mouse Resistance to Diet-Induced Dysmetabolism Is Associated with Altered Gut Microbiota

1
Département de Médecine, Université Laval, Québec, QC, G1V 0A6, Canada
2
Canada Excellence Research Chair on the Microbiome-Endocannabinoidome Axis in metabolic Health, Québec, QC, G1V 4G5, Canada
3
Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec, QC, G1V 4G5, Canada
4
Institut sur la Nutrition et les Aliments Fonctionnels, Université Laval, Québec, QC, G1V 0A6, Canada
5
Faculté des Sciences de L’agriculture et de L’alimentation, Université Laval, Québec, QC, G1V 0A6, Canada
6
Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria
7
Institute of Biomolecular Chemistry, National Research Council, 80078 Pozzuoli, NA, Italy
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Cells 2020, 9(12), 2705; https://doi.org/10.3390/cells9122705
Received: 19 November 2020 / Revised: 10 December 2020 / Accepted: 10 December 2020 / Published: 17 December 2020
Monoglyceride lipase (MGLL) regulates metabolism by catabolizing monoacylglycerols (MAGs), including the endocannabinoid 2-arachidonoyl glycerol (2-AG) and some of its bioactive congeners, to the corresponding free fatty acids. Mgll knockout mice (Mgll−/−) exhibit elevated tissue levels of MAGs in association with resistance to the metabolic and cardiovascular perturbations induced by a high fat diet (HFD). The gut microbiome and its metabolic function are disrupted in obesity in a manner modulated by 2-arachidonoyl glycerol (2-AG’s) main receptors, the cannabinoid CB1 receptors. We therefore hypothesized that Mgll−/− mice have an altered microbiome, that responds differently to diet-induced obesity from that of wild-type (WT) mice. We subjected mice to HFD and assessed changes in the microbiomes after 8 and 22 weeks. As expected, Mgll−/− mice showed decreased adiposity, improved insulin sensitivity, and altered circulating incretin/adipokine levels in response to HFD. Mgll−/− mice on a chow diet exhibited significantly higher levels of Hydrogenoanaerobacterium, Roseburia, and Ruminococcus than WT mice. The relative abundance of the Lactobacillaceae and Coriobacteriaceae and of the Lactobacillus, Enterorhabdus, Clostridium_XlVa, and Falsiporphyromonas genera was significantly altered by HFD in WT but not Mgll−/− mice. Differently abundant families were also associated with changes in circulating adipokine and incretin levels in HFD-fed mice. Some gut microbiota family alterations could be reproduced by supplementing 2-AG or MAGs in culturomics experiments carried out with WT mouse fecal samples. We suggest that the altered microbiome of Mgll−/− mice contributes to their obesity resistant phenotype, and results in part from increased levels of 2-AG and MAGs. View Full-Text
Keywords: monoglyceride lipase; endocannabinoids; 2-arachidonoyl glycerol; monoacylglycerols; microbiota monoglyceride lipase; endocannabinoids; 2-arachidonoyl glycerol; monoacylglycerols; microbiota
Show Figures

Figure 1

MDPI and ACS Style

Dione, N.; Lacroix, S.; Taschler, U.; Deschênes, T.; Abolghasemi, A.; Leblanc, N.; Di Marzo, V.; Silvestri, C. Mgll Knockout Mouse Resistance to Diet-Induced Dysmetabolism Is Associated with Altered Gut Microbiota. Cells 2020, 9, 2705. https://doi.org/10.3390/cells9122705

AMA Style

Dione N, Lacroix S, Taschler U, Deschênes T, Abolghasemi A, Leblanc N, Di Marzo V, Silvestri C. Mgll Knockout Mouse Resistance to Diet-Induced Dysmetabolism Is Associated with Altered Gut Microbiota. Cells. 2020; 9(12):2705. https://doi.org/10.3390/cells9122705

Chicago/Turabian Style

Dione, Niokhor, Sébastien Lacroix, Ulrike Taschler, Thomas Deschênes, Armita Abolghasemi, Nadine Leblanc, Vincenzo Di Marzo, and Cristoforo Silvestri. 2020. "Mgll Knockout Mouse Resistance to Diet-Induced Dysmetabolism Is Associated with Altered Gut Microbiota" Cells 9, no. 12: 2705. https://doi.org/10.3390/cells9122705

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop