Production of ROS by Gallic Acid Activates KDM2A to Reduce rRNA Transcription
1
Laboratory of Molecular and Cellular Biology, Faculty of Pharmacy, Takasaki University of Health and Welfare, Takasaki 370-0033, Japan
2
Education and Research Support Center, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan
3
Department of Biochemistry, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan
4
Laboratory of Molecular Design Chemistry, Faculty of Pharmacy, Takasaki University of Health and Welfare, Takasaki 370-0033, Japan
*
Author to whom correspondence should be addressed.
Cells 2020, 9(10), 2266; https://doi.org/10.3390/cells9102266
Received: 9 September 2020
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Revised: 4 October 2020
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Accepted: 8 October 2020
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Published: 10 October 2020
(This article belongs to the Special Issue Ribosome Biogenesis in Health and Disease)
Metformin, which is suggested to have anti-cancer effects, activates KDM2A to reduce rRNA transcription and proliferation of cancer cells. Thus, the specific activation of KDM2A may be applicable to the treatment of cancers. In this study, we screened a food-additive compound library to identify compounds that control cell proliferation. We found that gallic acid activated KDM2A to reduce rRNA transcription and cell proliferation in breast cancer MCF-7 cells. Gallic acid accelerated ROS production and activated AMPK. When ROS production or AMPK activity was inhibited, gallic acid did not activate KDM2A. These results suggest that both ROS production and AMPK activation are required for activation of KDM2A by gallic acid. Gallic acid did not reduce the succinate level, which was required for KDM2A activation by metformin. Metformin did not elevate ROS production. These results suggest that the activation of KDM2A by gallic acid includes mechanisms distinct from those by metformin. Therefore, signals from multiple intracellular conditions converge in KDM2A to control rRNA transcription. Gallic acid did not induce KDM2A-dependent anti-proliferation activity in non-tumorigenic MCF10A cells. These results suggest that the mechanism of KDM2A activation by gallic acid may be applicable to the treatment of breast cancers.
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Keywords:
rRNA transcription; breast cancer; KDM2A; gallic acid; ROS; histone demethylase
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MDPI and ACS Style
Tanaka, Y.; Obinata, H.; Konishi, A.; Yamagiwa, N.; Tsuneoka, M. Production of ROS by Gallic Acid Activates KDM2A to Reduce rRNA Transcription. Cells 2020, 9, 2266. https://doi.org/10.3390/cells9102266
AMA Style
Tanaka Y, Obinata H, Konishi A, Yamagiwa N, Tsuneoka M. Production of ROS by Gallic Acid Activates KDM2A to Reduce rRNA Transcription. Cells. 2020; 9(10):2266. https://doi.org/10.3390/cells9102266
Chicago/Turabian StyleTanaka, Yuji, Hideru Obinata, Akimitsu Konishi, Noriyuki Yamagiwa, and Makoto Tsuneoka. 2020. "Production of ROS by Gallic Acid Activates KDM2A to Reduce rRNA Transcription" Cells 9, no. 10: 2266. https://doi.org/10.3390/cells9102266
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