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Open AccessFeature PaperReview

Characterization of Cell Glycocalyx with Mass Spectrometry Methods

1
Department of Chemistry, University of California, Davis, CA 95616, USA
2
Department of Biochemistry, University of California, Davis, CA 95616, USA
*
Author to whom correspondence should be addressed.
Cells 2019, 8(8), 882; https://doi.org/10.3390/cells8080882
Received: 27 June 2019 / Revised: 5 August 2019 / Accepted: 12 August 2019 / Published: 13 August 2019
(This article belongs to the Special Issue Functional Glycomics)
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PDF [2905 KB, uploaded 13 August 2019]
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Abstract

The cell membrane plays an important role in protecting the cell from its extracellular environment. As such, extensive work has been devoted to studying its structure and function. Crucial intercellular processes, such as signal transduction and immune protection, are mediated by cell surface glycosylation, which is comprised of large biomolecules, including glycoproteins and glycosphingolipids. Because perturbations in glycosylation could result in dysfunction of cells and are related to diseases, the analysis of surface glycosylation is critical for understanding pathogenic mechanisms and can further lead to biomarker discovery. Different mass spectrometry-based techniques have been developed for glycan analysis, ranging from highly specific, targeted approaches to more comprehensive profiling studies. In this review, we summarized the work conducted for extensive analysis of cell membrane glycosylation, particularly those employing liquid chromatography with mass spectrometry (LC-MS) in combination with various sample preparation techniques. View Full-Text
Keywords: glycocalyx; glycomics; glycoproteomics; LC-MS/MS; cell membrane proteins; glycosphingolipids; C13 labeling glycocalyx; glycomics; glycoproteomics; LC-MS/MS; cell membrane proteins; glycosphingolipids; C13 labeling
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Li, Q.; Xie, Y.; Wong, M.; Lebrilla, C.B. Characterization of Cell Glycocalyx with Mass Spectrometry Methods. Cells 2019, 8, 882.

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