Tunicamycin Sensitivity-Suppression by High Gene Dosage Reveals New Functions of the Yeast Hog1 MAP Kinase
Abstract
:1. Introduction
2. Materials and Methods
2.1. Yeast Strains, Gene Disruptions, and Culture Conditions
2.2. Stress Assays
2.3. High Dosage Suppression Screening
2.4. Gene Expression
2.5. Physical Interactions and Construction of the Interaction Network
2.6. Polyprenol Determination
3. Results
3.1. Isolation of High Dosage-Gene Suppressors of Hog1Δ Tunicamycin Sensitivity
3.2. Genes Can Be Divided into General and HOG-Specific Suppressors of Tn Sensitivity
3.3. Inactivation of Some Suppressor Genes Confers Sensitivity to Tn
3.4. Suppressor Genes may Form an Interaction Network with HOG1
3.5. The Cis-Prenyltransferase Srt1 also Suppresses the Tn Sensitivity of the Hog1Δ Mutant
3.6. Hog1-Tn Sensitivity Is Enhanced by Overexpression of ERG9
3.7. The hog1Δ Strain Has Reduced Polyprenols Concentration
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene | Protein Name | Biological Process | Cellular Component |
---|---|---|---|
ALG7 | UDP-N-acetyl-glycosamine-1-P-transferase | Protein N-linked glycosylation | Endoplasmic reticulum |
GFA1 | Glutamine-fructose-6-phosphate amidotransferase | Cell wall biosynthesis | Unknown |
YOR1 | Plasma membrane ATP-binding cassette (ABC) transporter | Xenobiotic transport | Plasma membrane |
NAB6 | Putative RNA binding protein | Binds to poliA RNAs | Cytoplasm |
KIN2 | Serine/threonine protein kinase | Exocytosis | Plasma membrane |
KIN1 | Serine/threonine protein kinase | Exocytosis | Plasma membrane |
RER2 | Cis-prenyltranferase | ER to Golgi vesicle-mediated transport. Protein glycosilation | Endoplasmic reticulum |
RER1 | Retention in the endoplasmic reticulum | ER to Golgi vesicle-mediated transport. Protein retention in the ER lumen. Retrograde vesiclemediated transport, Golgi to ER | COPI-coated vesicle vacuole |
ECM13 | Protein induced by traetment with methoxypsoralen and UVA irradation | Cell wall biosynthesis? | Unknown |
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Hernández-Elvira, M.; Martínez-Gómez, R.; Domínguez-Martin, E.; Méndez, A.; Kawasaki, L.; Ongay-Larios, L.; Coria, R. Tunicamycin Sensitivity-Suppression by High Gene Dosage Reveals New Functions of the Yeast Hog1 MAP Kinase. Cells 2019, 8, 710. https://doi.org/10.3390/cells8070710
Hernández-Elvira M, Martínez-Gómez R, Domínguez-Martin E, Méndez A, Kawasaki L, Ongay-Larios L, Coria R. Tunicamycin Sensitivity-Suppression by High Gene Dosage Reveals New Functions of the Yeast Hog1 MAP Kinase. Cells. 2019; 8(7):710. https://doi.org/10.3390/cells8070710
Chicago/Turabian StyleHernández-Elvira, Mariana, Ricardo Martínez-Gómez, Eunice Domínguez-Martin, Akram Méndez, Laura Kawasaki, Laura Ongay-Larios, and Roberto Coria. 2019. "Tunicamycin Sensitivity-Suppression by High Gene Dosage Reveals New Functions of the Yeast Hog1 MAP Kinase" Cells 8, no. 7: 710. https://doi.org/10.3390/cells8070710