Next Article in Journal
The Catalase Gene Family in Cotton: Genome-Wide Characterization and Bioinformatics Analysis
Next Article in Special Issue
Low Molecular Mass Myocardial Hyaluronan in Human Hypertrophic Cardiomyopathy
Previous Article in Journal
Biochemical Differences in Cerebrospinal Fluid between Secondary Progressive and Relapsing–Remitting Multiple Sclerosis
Previous Article in Special Issue
Tumor Extracellular Matrix Remodeling: New Perspectives as a Circulating Tool in the Diagnosis and Prognosis of Solid Tumors
Article Menu

Export Article

Open AccessArticle
Cells 2019, 8(2), 85; https://doi.org/10.3390/cells8020085

Transcriptome-Wide Analysis of Human Chondrocyte Expansion on Synoviocyte Matrix

1
Orthopedic Surgery, Baylor College of Medicine, Houston, TX 77030, USA
2
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
*
Author to whom correspondence should be addressed.
Received: 9 December 2018 / Revised: 18 January 2019 / Accepted: 21 January 2019 / Published: 24 January 2019
(This article belongs to the Special Issue Extracellular Matrix Remodeling)
Full-Text   |   PDF [3280 KB, uploaded 24 January 2019]   |  
  |   Review Reports

Abstract

Human chondrocytes are expanded and used in autologous chondrocyte implantation techniques and are known to rapidly de-differentiate in culture. These chondrocytes, when cultured on tissue culture plastic (TCP), undergo both phenotypical and morphological changes and quickly lose the ability to re-differentiate to produce hyaline-like matrix. Growth on synoviocyte-derived extracellular matrix (SDECM) reduces this de-differentiation, allowing for more than twice the number of population doublings (PD) whilst retaining chondrogenic capacity. The goal of this study was to apply RNA sequencing (RNA-Seq) analysis to examine the differences between TCP-expanded and SDECM-expanded human chondrocytes. Human chondrocytes from three donors were thawed from primary stocks and cultured on TCP flasks or on SDECM-coated flasks at physiological oxygen tension (5%) for 4 passages. During log expansion, RNA was extracted from the cell layer (70–90% confluence) at passages 1 and 4. Total RNA was column-purified and DNAse-treated before quality control analysis and next-generation RNA sequencing. Significant effects on gene expression were observed due to both culture surface and passage number. These results offer insight into the mechanism of how SDECM provides a more chondrogenesis-preserving environment for cell expansion, the transcriptome-wide changes that occur with culture, and potential mechanisms for further enhancement of chondrogenesis-preserving growth. View Full-Text
Keywords: chondrocyte RNA-Seq; dedifferentiation; chondrogenesis; synoviocyte matrix; physioxia; RNA-Seq; cell senescence chondrocyte RNA-Seq; dedifferentiation; chondrogenesis; synoviocyte matrix; physioxia; RNA-Seq; cell senescence
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Kean, T.J.; Ge, Z.; Li, Y.; Chen, R.; Dennis, J.E. Transcriptome-Wide Analysis of Human Chondrocyte Expansion on Synoviocyte Matrix. Cells 2019, 8, 85.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Cells EISSN 2073-4409 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top