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Open AccessArticle

Extrachromosomal Histone H2B Contributes to the Formation of the Abscission Site for Cell Division

1
Unit of Cellular Networks and Molecular Therapeutic Targets, IRCCS-Regina Elena National Cancer Institute, 00144 Rome, Italy
2
Institutes of Molecular Biology and Pathology (IBPM), National Research Council (CNR), c/o Sapienza University, 00185 Rome, Italy
*
Authors to whom correspondence should be addressed.
Cells 2019, 8(11), 1391; https://doi.org/10.3390/cells8111391
Received: 8 October 2019 / Revised: 25 October 2019 / Accepted: 31 October 2019 / Published: 5 November 2019
(This article belongs to the Special Issue Molecular Factors and Mechanisms Involved in Cytokinesis)
Histones are constitutive components of nucleosomes and key regulators of chromatin structure. We previously observed that an extrachromosomal histone H2B (ecH2B) localizes at the intercellular bridge (ICB) connecting the two daughter cells during cytokinesis independently of DNA and RNA. Here, we show that ecH2B binds and colocalizes with CHMP4B, a key component of the ESCRT-III machinery responsible for abscission, the final step of cell division. Abscission requires the formation of an abscission site at the ICB where the ESCRT-III complex organizes into narrowing cortical helices that drive the physical separation of sibling cells. ecH2B depletion does not prevent membrane cleavage rather results in abscission delay and accumulation of abnormally long and thin ICBs. In the absence of ecH2B, CHMP4B and other components of the fission machinery, such as IST1 and Spastin, are recruited to the ICB and localize at the midbody. However, in the late stage of abscission, these fission factors fail to re-localize at the periphery of the midbody and the abscission site fails to form. These results show that extrachromosomal activity of histone H2B is required in the formation of the abscission site and the proper localization of the fission machinery. View Full-Text
Keywords: extrachromosomal histone H2B; abscission site; CHMP4B; ESCRT-III fission machinery extrachromosomal histone H2B; abscission site; CHMP4B; ESCRT-III fission machinery
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Monteonofrio, L.; Valente, D.; Rinaldo, C.; Soddu, S. Extrachromosomal Histone H2B Contributes to the Formation of the Abscission Site for Cell Division. Cells 2019, 8, 1391.

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