Next Article in Journal
Relevance of Autophagy in Parenchymal and Non-Parenchymal Liver Cells for Health and Disease
Previous Article in Journal
Zonisamide Administration Improves Fatty Acid β-Oxidation in Parkinson’s Disease
Article Menu

Export Article

Open AccessArticle
Cells 2019, 8(1), 15; https://doi.org/10.3390/cells8010015

A Role for Circular Non-Coding RNAs in the Pathogenesis of Sporadic Parathyroid Adenomas and the Impact of Gender-Specific Epigenetic Regulation

1
1st Department of Propaedeutic Internal Medicine, National and Kapodistrian University of Athens, School of Medicine, Laikon General Hospital, Athens 11527, Greece
2
Pathology Department, Faculty of Medicine, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece
3
3rd Department of Surgery, AHEPA Univ. Hospital, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece
4
Laboratory of Clinical and Molecular Endocrinology, 1st Department of Internal Medicine, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece
5
1st Department of Surgery, AHEPA Univ. Hospital, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece
*
Author to whom correspondence should be addressed.
Received: 15 November 2018 / Revised: 22 December 2018 / Accepted: 24 December 2018 / Published: 30 December 2018
Full-Text   |   PDF [1488 KB, uploaded 30 December 2018]   |  

Abstract

Epigenetic changes, including altered small non-coding RNAs, appear to be implicated in the pathogenesis of sporadic parathyroid adenomas (PAs). In this study, we investigated the circular RNAs (circRNAs) expression profile in sporadic PAs. Sixteen tissue samples of sporadic PAs, and four samples of normal parathyroid tissue (NPT) were investigated. Sample preparation and microarray hybridization were performed based on the Arraystar’s standard protocols, and circRNAs sequences were predicted by bioinformatics tools. We identified 35 circRNAs that were differentially expressed in sporadic PAs compared to NPT; 22 were upregulated, and 13 were downregulated, according to the pre-defined thresholds of fold-change > 2.0 and p < 0.05. In the subgroup analysis of PAs from male patients (n = 7) compared to PAs from female patients (n = 9), we also find a different expression profile. In particular, 19 circRNAs were significantly upregulated, and four circRNAs were significantly downregulated in male patients, compared to female counterparts. We show here for the first time a differential circRNA expression pattern in sporadic PAs compared to NPT, and a different expression profile in PA samples from male compared to female patients, suggesting an epigenetic role in the PA pathogenesis, and also an effect of gender in the epigenetic regulation of PAs. View Full-Text
Keywords: parathyroid adenomas; pathogenesis; epigenetics; non-coding RNAs; circular RNAs parathyroid adenomas; pathogenesis; epigenetics; non-coding RNAs; circular RNAs
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Yavropoulou, M.P.; Poulios, C.; Michalopoulos, N.; Gatzou, A.; Chrisafi, S.; Mantalovas, S.; Papavramidis, T.; Daskalaki, E.; Sofou, E.; Kotsa, K.; Kesisoglou, I.; Zebekakis, P.; Yovos, J.G. A Role for Circular Non-Coding RNAs in the Pathogenesis of Sporadic Parathyroid Adenomas and the Impact of Gender-Specific Epigenetic Regulation. Cells 2019, 8, 15.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Cells EISSN 2073-4409 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top