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Article

Self-Assembled Permanent Micro-Magnets in a Polymer-Based Microfluidic Device for Magnetic Cell Sorting

1
CNRS, INSA Lyon, Ecole Centrale de Lyon, CPE Lyon, INL, UMR5270, University Lyon, Université Claude Bernard Lyon 1, 69622 Villeurbanne, France
2
CNRS, UMR5306 Institut Lumière Matière, University Lyon, Université Claude Bernard Lyon 1, 69100 Villeurbanne, France
3
Laboratoire de Biochimie Et Biologie Moléculaire, Groupe Hospitalier Sud, Hospices Civils de Lyon, 69495 Pierre Bénite, France
4
CNRS, INSA Lyon, CPE Lyon, CNRS, INL, UMR5270, University Lyon, Ecole Centrale de Lyon, Université Claude Bernard Lyon 1, 69130 Ecully, France
*
Authors to whom correspondence should be addressed.
Academic Editors: Vitalii Zablotskii and Xin Zhang
Cells 2021, 10(7), 1734; https://doi.org/10.3390/cells10071734
Received: 26 May 2021 / Revised: 25 June 2021 / Accepted: 5 July 2021 / Published: 9 July 2021
(This article belongs to the Collection Magnetic Fields and Cells)
Magnetophoresis-based microfluidic devices offer simple and reliable manipulation of micro-scale objects and provide a large panel of applications, from selective trapping to high-throughput sorting. However, the fabrication and integration of micro-scale magnets in microsystems involve complex and expensive processes. Here we report on an inexpensive and easy-to-handle fabrication process of micrometer-scale permanent magnets, based on the self-organization of NdFeB particles in a polymer matrix (polydimethylsiloxane, PDMS). A study of the inner structure by X-ray tomography revealed a chain-like organization of the particles leading to an array of hard magnetic microstructures with a mean diameter of 4 µm. The magnetic performance of the self-assembled micro-magnets was first estimated by COMSOL simulations. The micro-magnets were then integrated into a microfluidic device where they act as micro-traps. The magnetic forces exerted by the micro-magnets on superparamagnetic beads were measured by colloidal probe atomic force microscopy (AFM) and in operando in the microfluidic system. Forces as high as several nanonewtons were reached. Adding an external millimeter-sized magnet allowed target magnetization and the interaction range to be increased. Then, the integrated micro-magnets were used to study the magnetophoretic trapping efficiency of magnetic beads, providing efficiencies of 100% at 0.5 mL/h and 75% at 1 mL/h. Finally, the micro-magnets were implemented for cell sorting by performing white blood cell depletion. View Full-Text
Keywords: magnetophoresis; micro-magnets; polymer composite; microfluidic devices; particle separation magnetophoresis; micro-magnets; polymer composite; microfluidic devices; particle separation
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MDPI and ACS Style

Descamps, L.; Audry, M.-C.; Howard, J.; Mekkaoui, S.; Albin, C.; Barthelemy, D.; Payen, L.; Garcia, J.; Laurenceau, E.; Le Roy, D.; Deman, A.-L. Self-Assembled Permanent Micro-Magnets in a Polymer-Based Microfluidic Device for Magnetic Cell Sorting. Cells 2021, 10, 1734. https://doi.org/10.3390/cells10071734

AMA Style

Descamps L, Audry M-C, Howard J, Mekkaoui S, Albin C, Barthelemy D, Payen L, Garcia J, Laurenceau E, Le Roy D, Deman A-L. Self-Assembled Permanent Micro-Magnets in a Polymer-Based Microfluidic Device for Magnetic Cell Sorting. Cells. 2021; 10(7):1734. https://doi.org/10.3390/cells10071734

Chicago/Turabian Style

Descamps, Lucie, Marie-Charlotte Audry, Jordyn Howard, Samir Mekkaoui, Clément Albin, David Barthelemy, Léa Payen, Jessica Garcia, Emmanuelle Laurenceau, Damien Le Roy, and Anne-Laure Deman. 2021. "Self-Assembled Permanent Micro-Magnets in a Polymer-Based Microfluidic Device for Magnetic Cell Sorting" Cells 10, no. 7: 1734. https://doi.org/10.3390/cells10071734

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