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Article

Novel Interplay between p53 and HO-1 in Embryonic Stem Cells

1
CONICET, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Universidad de Buenos Aires, Buenos Aires C1428EGA, Argentina
2
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires C1428EGA, Argentina
3
Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires C1428EGA, Argentina
*
Authors to whom correspondence should be addressed.
Cells 2021, 10(1), 35; https://doi.org/10.3390/cells10010035
Received: 30 October 2020 / Revised: 22 December 2020 / Accepted: 24 December 2020 / Published: 29 December 2020
(This article belongs to the Special Issue New Insight into Heme Oxygenases: Beyond Heme Degradation)
Stem cells genome safeguarding requires strict oxidative stress control. Heme oxygenase-1 (HO-1) and p53 are relevant components of the cellular defense system. p53 controls cellular response to multiple types of harmful stimulus, including oxidative stress. Otherwise, besides having a protective role, HO-1 is also involved in embryo development and in embryonic stem (ES) cells differentiation. Although both proteins have been extensively studied, little is known about their relationship in stem cells. The aim of this work is to explore HO-1-p53 interplay in ES cells. We studied HO-1 expression in p53 knockout (KO) ES cells and we found that they have higher HO-1 protein levels but similar HO-1 mRNA levels than the wild type (WT) ES cell line. Furthermore, cycloheximide treatment increased HO-1 abundance in p53 KO cells suggesting that p53 modulates HO-1 protein stability. Notably, H2O2 treatment did not induce HO-1 expression in p53 KO ES cells. Finally, SOD2 protein levels are also increased while Sod2 transcripts are not in KO cells, further suggesting that the p53 null phenotype is associated with a reinforcement of the antioxidant machinery. Our results demonstrate the existence of a connection between p53 and HO-1 in ES cells, highlighting the relationship between these stress defense pathways. View Full-Text
Keywords: heme oxygenase-1; p53; embryonic stem cells; gene expression regulation heme oxygenase-1; p53; embryonic stem cells; gene expression regulation
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MDPI and ACS Style

Toro, A.; Anselmino, N.; Solari, C.; Francia, M.; Oses, C.; Sanchis, P.; Bizzotto, J.; Vazquez Echegaray, C.; Petrone, M.V.; Levi, V.; Vazquez, E.; Guberman, A. Novel Interplay between p53 and HO-1 in Embryonic Stem Cells. Cells 2021, 10, 35. https://doi.org/10.3390/cells10010035

AMA Style

Toro A, Anselmino N, Solari C, Francia M, Oses C, Sanchis P, Bizzotto J, Vazquez Echegaray C, Petrone MV, Levi V, Vazquez E, Guberman A. Novel Interplay between p53 and HO-1 in Embryonic Stem Cells. Cells. 2021; 10(1):35. https://doi.org/10.3390/cells10010035

Chicago/Turabian Style

Toro, Ayelén, Nicolás Anselmino, Claudia Solari, Marcos Francia, Camila Oses, Pablo Sanchis, Juan Bizzotto, Camila Vazquez Echegaray, María V. Petrone, Valeria Levi, Elba Vazquez, and Alejandra Guberman. 2021. "Novel Interplay between p53 and HO-1 in Embryonic Stem Cells" Cells 10, no. 1: 35. https://doi.org/10.3390/cells10010035

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