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Development of ent-kaurene Oxidase-Based Conserved Intron Spanning Primers for Species Identification in the Genus Poa (Poaceae; Bluegrass)

1
United States Department of Agriculture, Williams Hall 1680 Madison Ave., Wooster, OH 44691, USA
2
Texas AgriLife Research and Extension Center-Dallas, Texas A&M System, 17360 Coit Road, Dallas, TX 75252, USA
3
Department of Plant Science, Pennsylvania State University, University Park, PA 16802, USA
*
Author to whom correspondence should be addressed.
Agronomy 2018, 8(5), 58; https://doi.org/10.3390/agronomy8050058
Received: 30 March 2018 / Revised: 17 April 2018 / Accepted: 19 April 2018 / Published: 24 April 2018
(This article belongs to the Special Issue Turfgrass Biology, Genetics, and Breeding)
Interspecific hybridization has been attempted to combine the heat and drought of Poa arachnifera Torr. with the turf quality characteristics of several Poa species. Confirmation of an F1 hybrid through morphological analysis of vegetative and flowering characteristics is often time consuming and ambiguous. Ent-kaurene oxidase (KO) has been sequenced in rice, barley, and wheat. In rice, each of the five copies of KO gene has unique lengths for the first intron. Conserved intron spanning primers (CISP) can be used as a DNA marker to exploit variations of intron lengths that flank conserved gene sequences. In the present study, we developed CISP to sequence partial genomic fragments of the KO gene from seven Poa species. Through sequence analysis, species-specific primers were also developed to produce co-dominant markers that can be used to identify interspecific hybrids between Texas bluegrass and six other Poa species used in the present study. View Full-Text
Keywords: Texas bluegrass; turfgrass; molecular marker Texas bluegrass; turfgrass; molecular marker
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LaMantia, J.M.; Chandra, A.; Huff, D.R. Development of ent-kaurene Oxidase-Based Conserved Intron Spanning Primers for Species Identification in the Genus Poa (Poaceae; Bluegrass). Agronomy 2018, 8, 58.

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