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Article

A Global Screening Assay to Select for Maize Phenotypes with a High Tolerance or Resistance to Fusarium verticillioides (Sacc.) Nirenberg Rots

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Instituto Politécnico Nacional, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Sinaloa (CIIDIR-IPN Unidad Sinaloa), Laboratorio de Fitomejoramiento Molecular, Blvd. Juan de Dios Bátiz Paredes no. 250, Col. San Joachín, Guasave 81101, Sinaloa, Mexico
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Híbridos Lobo SA de CV, Departamento de Investigación, Av. Guerrero 330 Int 2, Cuauhtémoc 31500, Chihuahua, Mexico
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Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Cluster BioMimic®, Carretera Antigua a Coatepec 351, Congregación el Haya, Xalapa 91070, Veracruz, Mexico
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Departamento de Agronomia, Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal
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Syngenta Ghent Innovation Center, Devgen NV, Technologiepark 30, 9052 Gent-Zwijnaarde, Belgium
*
Author to whom correspondence should be addressed.
These authors contributed equally to this manuscript.
Agronomy 2020, 10(12), 1990; https://doi.org/10.3390/agronomy10121990
Received: 11 November 2020 / Revised: 9 December 2020 / Accepted: 10 December 2020 / Published: 18 December 2020
(This article belongs to the Special Issue Maize Breeding for Alternative and Multiple Uses)
Fusarium verticillioides (Sacc.) Nirenberg (Fv) causes rots in maize around the world and produces mycotoxins that contaminate grains, making this species a significant health concern for both animals and humans. One of the best approaches to address rots is to identify highly tolerant or resistant genotypes that can be used for genetic improvement. The aim of the study was to evaluate dose-response assays to tolerance or resistance for Fv rots throughout the maize life cycle. These tests assessed the effects of Fv during post-germination development and the seedling (V2) stage by seed infection, the plantlet (V4) stage by substrate infection, and in the reproductive phase in maize stalks (R2 stage) and ears (R6 stage) by R1 stage inoculation. In all assays, the doses were effective at distinguishing contrasting phenotypes. Severity, root fresh weight, and aerial length were the most informative parameters at the V2 and V4 stages. Evaluation of the stalk necrosis area between and within the internodes of susceptible genotypes revealed significant differences among doses, and a positive correlation between necrosis and conidia concentration was observed in internodes. Injecting eight million conidia in the ear was sufficient for selecting different phenotypes. A total of 85% of the genotypes conserved their same capacity to respond to Fv infection throughout the maize life cycle, so that screening at the early vegetative stage (e.g., V2) could be useful for distinguishing contrasting phenotypes in the reproductive stage. Implementing these screening assays in a maize breeding program could be valuable for classifying the degrees of resilience of maize germplasms to Fv rots. This global screening has the potential to be employed to select against other Fusarium species. View Full-Text
Keywords: fusariosis; maize breeding; Zea mays L. fusariosis; maize breeding; Zea mays L.
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MDPI and ACS Style

Román, S.G.; Quiroz-Chávez, J.; Villalobos, M.; Urías-Gutiérrez, V.; Nava-Pérez, E.; Ruíz-May, E.; Singh, R.K.; Sharma, L.; Quiroz-Figueroa, F.R. A Global Screening Assay to Select for Maize Phenotypes with a High Tolerance or Resistance to Fusarium verticillioides (Sacc.) Nirenberg Rots. Agronomy 2020, 10, 1990. https://doi.org/10.3390/agronomy10121990

AMA Style

Román SG, Quiroz-Chávez J, Villalobos M, Urías-Gutiérrez V, Nava-Pérez E, Ruíz-May E, Singh RK, Sharma L, Quiroz-Figueroa FR. A Global Screening Assay to Select for Maize Phenotypes with a High Tolerance or Resistance to Fusarium verticillioides (Sacc.) Nirenberg Rots. Agronomy. 2020; 10(12):1990. https://doi.org/10.3390/agronomy10121990

Chicago/Turabian Style

Román, Shamir G., Jesús Quiroz-Chávez, Miguel Villalobos, Vianey Urías-Gutiérrez, Eusebio Nava-Pérez, Eliel Ruíz-May, Rupesh K. Singh, Lav Sharma, and Francisco R. Quiroz-Figueroa 2020. "A Global Screening Assay to Select for Maize Phenotypes with a High Tolerance or Resistance to Fusarium verticillioides (Sacc.) Nirenberg Rots" Agronomy 10, no. 12: 1990. https://doi.org/10.3390/agronomy10121990

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