Next Article in Journal
Block Copolymer Membranes from Polystyrene-b-poly(solketal methacrylate) (PS-b-PSMA) and Amphiphilic Polystyrene-b-poly(glyceryl methacrylate) (PS-b-PGMA)
Previous Article in Journal
Multifunctional Polymer Nanoparticles for Dual Drug Release and Cancer Cell Targeting
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Polymers 2017, 9(6), 215;

Primary Hepatocytes Cultured on a Fiber-Embedded PDMS Chip to Study Drug Metabolism

1,2,* , 1
College of Food Science, Sichuan Agricultural University, Yaan 625014, China
School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China
Author to whom correspondence should be addressed.
Academic Editor: Patrick van Rijn
Received: 27 April 2017 / Revised: 25 May 2017 / Accepted: 7 June 2017 / Published: 10 June 2017
Full-Text   |   PDF [3030 KB, uploaded 10 June 2017]   |  


In vitro drug screening using reliable and predictable liver models remains a challenge. The identification of an ideal biological substrate is essential to maintain hepatocyte functions during in vitro culture. Here, we developed a fiber-embedded polydimethylsiloxane (PDMS) chip to culture hepatocytes. Hepatocyte spheroids formed in this device were subjected to different flow rates, of which a flow rate of 50 μL/min provided the optimal microenvironment for spheroid formation, maintained significantly higher rates of albumin and urea synthesis, yielded higher CYP3A1 (cytochrome P450 3A1) and CYP2C11 (cytochrome P450 2C11) enzyme activities for metabolism, and demonstrated higher expression levels of liver-specific genes. In vitro metabolism tests on tolbutamide and testosterone by hepatocytes indicated predicted clearance rates of 1.98 ± 0.43 and 40.80 ± 10.13 mL/min/kg, respectively, which showed a good in vitro–in vivo correspondence. These results indicate that this system provides a strategy for the construction of functional engineered liver tissue that can be used to study drug metabolism. View Full-Text
Keywords: fibers; microfluidic chips; hepatocytes; drug fibers; microfluidic chips; hepatocytes; drug

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Liu, Y.; Hu, K.; Wang, Y. Primary Hepatocytes Cultured on a Fiber-Embedded PDMS Chip to Study Drug Metabolism. Polymers 2017, 9, 215.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Polymers EISSN 2073-4360 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top