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Preparation and Characterization of Thermoresponsive Poly(N-Isopropylacrylamide) for Cell Culture Applications

by Lei Yang 1,*, Xiaoguang Fan 2,*, Jing Zhang 1 and Jia Ju 1
1
College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, China
2
College of Engineering, Shenyang Agricultural University, Shenyang 110866, China
*
Authors to whom correspondence should be addressed.
Polymers 2020, 12(2), 389; https://doi.org/10.3390/polym12020389
Received: 7 November 2019 / Revised: 7 January 2020 / Accepted: 17 January 2020 / Published: 9 February 2020
(This article belongs to the Section Polymer Processing and Performance)
Poly(N-isopropylacrylamide) (PNIPAAm) is a typical thermoresponsive polymer used widely and studied deeply in smart materials, which is attractive and valuable owing to its reversible and remote “on–off” behavior adjusted by temperature variation. PNIPAAm usually exhibits opposite solubility or wettability across lower critical solution temperature (LCST), and it is readily functionalized making it available in extensive applications. Cell culture is one of the most prospective and representative applications. Active attachment and spontaneous detachment of targeted cells are easily tunable by surface wettability changes and volume phase transitions of PNIPAAm modified substrates with respect to ambient temperature. The thermoresponsive culture platforms and matching thermal-liftoff method can effectively substitute for the traditional cell harvesting ways like enzymatic hydrolysis and mechanical scraping, and will improve the stable and high quality of recovered cells. Therefore, the establishment and detection on PNIPAAm based culture systems are of particular importance. This review covers the important developments and recommendations for future work of the preparation and characterization of temperature-responsive substrates based on PNIPAAm and analogues for cell culture applications. View Full-Text
Keywords: poly(N-isopropylacrylamide); thermosensitive polymer; thermoresponsive culture platform; cell culture; polymer characterization poly(N-isopropylacrylamide); thermosensitive polymer; thermoresponsive culture platform; cell culture; polymer characterization
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MDPI and ACS Style

Yang, L.; Fan, X.; Zhang, J.; Ju, J. Preparation and Characterization of Thermoresponsive Poly(N-Isopropylacrylamide) for Cell Culture Applications. Polymers 2020, 12, 389.

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