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A Universal Photochemical Method to Prepare Carbohydrate Sensors Based on Perfluorophenylazide Modified Polydopamine for Study of Carbohydrate-Lectin Interactions by QCM Biosensor

1
Shannxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A & F University, Yangling 712100, China
2
School of Pharmaceutical Sciences, Zhengzhou University, 100 Kexue Avenue, Zhengzhou 450001, China
*
Authors to whom correspondence should be addressed.
Polymers 2019, 11(6), 1023; https://doi.org/10.3390/polym11061023
Received: 8 May 2019 / Revised: 4 June 2019 / Accepted: 6 June 2019 / Published: 10 June 2019
(This article belongs to the Special Issue Polymer Based Bio-Sensors)
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Abstract

A universal photochemical method to prepare carbohydrate sensors based on perfluorophenylazide (PFPA) modified polydopamine (PDA) for the study of carbohydrate–lectin interactions by a quartz crystal microbalance (QCM) biosensor was developed. The PFPA was immobilized on PDA-coated gold sensors via Schiff base reactions. Upon light irradiation, the underivatized carbohydrates were inserted into the sensor surface, including mannose, galactose, fucose and N-acetylglucosamine (GlcNAc). Carbohydrate sensors were evaluated for the binding to a series of plant lectins. A kinetic study of the interactions between mannose and concanavalin A (Con A), fucose and Ulex europaeus agglutinin I (UEA-I) were performed. This method can eliminate the tedious modification of carbohydrates, improve the experimental efficiency, and reduce the experimental cost, which is of great significance for the development of QCM biosensors and the study of biomolecular interactions. View Full-Text
Keywords: perfluorophenylazide; polydopamine; underivatized carbohydrates; QCM biosensor; carbohydrate-lectin interactions perfluorophenylazide; polydopamine; underivatized carbohydrates; QCM biosensor; carbohydrate-lectin interactions
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Guo, L.; Chao, S.; Huang, P.; Lv, X.; Song, Q.; Wu, C.; Pei, Y.; Pei, Z. A Universal Photochemical Method to Prepare Carbohydrate Sensors Based on Perfluorophenylazide Modified Polydopamine for Study of Carbohydrate-Lectin Interactions by QCM Biosensor. Polymers 2019, 11, 1023.

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