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Open AccessArticle

Human Adipose-Derived Stem Cell Secreted Extracellular Matrix Incorporated into Electrospun Poly(Lactic-co-Glycolic Acid) Nanofibrous Dressing for Enhancing Wound Healing

1
Department of Chemical Engineering, College of Engineering, National Taiwan University, Taipei 106, Taiwan
2
School of Dental Technology, Taipei Medical University, Taipei 106, Taiwan
3
Department of Surgery, National Taiwan University Hospital, Taipei 100, Taiwan
*
Author to whom correspondence should be addressed.
Polymers 2019, 11(10), 1609; https://doi.org/10.3390/polym11101609
Received: 20 August 2019 / Revised: 26 September 2019 / Accepted: 29 September 2019 / Published: 3 October 2019
(This article belongs to the Special Issue Polymeric Colloidal Materials for Biomedical Applications)
Wound dressing, which prevents dehydration and provides a physical barrier against infection to wound beds, can improve wound healing. The interactions between extracellular matrix (ECM) and growth factors is critical to the healing process. Electrospun nanofibers are promising templates for wound dressings due to the structure similarity to ECM of skin. Otherwise, the ECM secreted by human adipose-derived stem cells (hASCs) is rich in growth factors known to enhance wound healing. Accordingly, we propose that the PLGA nanofibrous template incorporated with hASCs-secreted ECM may enhance wound healing. In this study, PLGA nanofibrous matrixes with an aligned or a random structure were prepared by electrospinning. Human ASCs cultured on the aligned matrix had a better viability and produced a larger amount of ECM relative to that of random one. After 7 days’ cultivation, the hASCs on aligned PLGA substrates underwent decellularization to fabricate cECM/PLGA dressings. By using immunohistochemical staining against F-actin and cell nucleus, the removal of cellular components was verified. However, the type I collagen and laminin were well preserved on the cECM/PLGA nanofibrous matrixes. In addition, this substrate was hydrophilic, with appropriate mechanical strength to act as a wound dressing. The L929 fibroblasts had good activity, survival and proliferation on the cECM/PLGA meshes. In addition, the cECM/PLGA nanofibrous dressings improved the wound healing of surgically created full-thickness skin excision in a mouse model. This hASCs-secreted ECM incorporated into electrospun PLGA nanofibrous could be a promising dressing for enhancing wound healing. View Full-Text
Keywords: wound healing; electrospinning; extracellular matrix; human adipose-derived stem cells wound healing; electrospinning; extracellular matrix; human adipose-derived stem cells
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MDPI and ACS Style

Tang, K.-C.; Yang, K.-C.; Lin, C.-W.; Chen, Y.-K.; Lu, T.-Y.; Chen, H.-Y.; Cheng, N.-C.; Yu, J. Human Adipose-Derived Stem Cell Secreted Extracellular Matrix Incorporated into Electrospun Poly(Lactic-co-Glycolic Acid) Nanofibrous Dressing for Enhancing Wound Healing. Polymers 2019, 11, 1609.

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