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Open AccessReview

Protein–Polyelectrolyte Interaction: Thermodynamic Analysis Based on the Titration Method

by 1,‡, 1,‡, 2,*, 1,3,4 and 1,3,4,*
1
State-Key Laboratory of Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China
2
Institute of Vascular Surgery, Fudan University, 180 Fenglin road, Shanghai 200032, China
3
International Joint Research Center of Green Energy Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China
4
Engineering Research Center of Xinjiang Bingtuan of Materials Chemical Engineering, Shihezi University, Xinjiang 832000, China
*
Authors to whom correspondence should be addressed.
This short review is in memory of my PhD advisor Prof. Paul Dubin who passed away in May, 2018.
Xiaohan Wang and Kai Zheng contributed equally this this work.
Polymers 2019, 11(1), 82; https://doi.org/10.3390/polym11010082
Received: 28 November 2018 / Revised: 26 December 2018 / Accepted: 2 January 2019 / Published: 7 January 2019
This review discussed the mechanisms including theories and binding stages concerning the protein–polyelectrolyte (PE) interaction, as well as the applications for both complexation and coacervation states of protein–PE pairs. In particular, this review focused on the applications of titration techniques, that is, turbidimetric titration and isothermal titration calorimetry (ITC), in understanding the protein–PE binding process. To be specific, by providing thermodynamic information such as pHc, pHφ, binding constant, entropy, and enthalpy change, titration techniques could shed light on the binding affinity, binding stoichiometry, and driving force of the protein–PE interaction, which significantly guide the applications by utilization of these interactions. Recent reports concerning interactions between proteins and different types of polyelectrolytes, that is, linear polyelectrolytes and polyelectrolyte modified nanoparticles, are summarized with their binding differences systematically discussed and compared based on the two major titration techniques. We believe this short review could provide valuable insight in the understanding of the structure–property relationship and the design of applied biomedical PE-based systems with optimal performance. View Full-Text
Keywords: polyelectrolyte; complexation; electrostatics; thermodynamic analysis; isothermal titration calorimetry polyelectrolyte; complexation; electrostatics; thermodynamic analysis; isothermal titration calorimetry
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Wang, X.; Zheng, K.; Si, Y.; Guo, X.; Xu, Y. Protein–Polyelectrolyte Interaction: Thermodynamic Analysis Based on the Titration Method . Polymers 2019, 11, 82.

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