Immobilization of Pyrroloquinoline Quinone-Dependent Alcohol Dehydrogenase with a Polyion Complex and Redox Polymer for a Bioanode

Sakurada, Yuki; Takeda, Kouta; Ohno, Hiroyuki; Nakamura, Nobuhumi

doi:10.3390/catal7100296

Open AccessArticle

Immobilization of Pyrroloquinoline Quinone-Dependent Alcohol Dehydrogenase with a Polyion Complex and Redox Polymer for a Bioanode

by

Yuki Sakurada

,

Kouta Takeda

,

Hiroyuki Ohno

and

Nobuhumi Nakamura

^*

Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-8588, Japan

^*

Author to whom correspondence should be addressed.

Catalysts 2017, 7(10), 296; https://doi.org/10.3390/catal7100296

Submission received: 28 August 2017 / Revised: 28 September 2017 / Accepted: 30 September 2017 / Published: 3 October 2017

(This article belongs to the Special Issue Biocatalysis and Biotransformations)

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Abstract

A bioanode for ethanol oxidation was prepared by immobilizing the recombinant pyrroloquinoline quinone (PQQ)-dependent alcohol dehydrogenase from Pseudomonas putida KT 2440 (PpADH) with polyion complex (PIC) and redox polymer. The PIC based on poly-l-lysine (PLL) and poly-l-glutamic acid (PGA) was suitable for immobilizing PpADH on the electrode. PpADH was immobilized using only one redox polymer, aminoferrocene, which was attached to the PGA backbone (PGA-AmFc) on the electrode. The anodic current density at 0.6 V (vs. Ag/AgCl) was 22.6 μA·cm⁻². However, when the number of the cycles was increased, the catalytic current drastically decreased. PpADH was immobilized using PGA-AmFc and PIC on the electrode. The anodic current density at 0.5 V (vs. Ag/AgCl) was 47.3 μA·cm⁻², and the performance maintained 74% of the initial value after five cycles. This result indicated that the combination of PIC and PGA-AmFc was suitable for the immobilization of PpADH on the electrode. In addition, the long-term stability and catalytic current density were improved by using the large surface area afforded by the gold nanoparticles.

Keywords: biofuel cells; quinoproteins; pyrroloquinoline quinone; alcohol dehydrogenase; polyion complex; redox mediator; gold nanoparticles

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MDPI and ACS Style

Sakurada, Y.; Takeda, K.; Ohno, H.; Nakamura, N. Immobilization of Pyrroloquinoline Quinone-Dependent Alcohol Dehydrogenase with a Polyion Complex and Redox Polymer for a Bioanode. Catalysts 2017, 7, 296. https://doi.org/10.3390/catal7100296

AMA Style

Sakurada Y, Takeda K, Ohno H, Nakamura N. Immobilization of Pyrroloquinoline Quinone-Dependent Alcohol Dehydrogenase with a Polyion Complex and Redox Polymer for a Bioanode. Catalysts. 2017; 7(10):296. https://doi.org/10.3390/catal7100296

Chicago/Turabian Style

Sakurada, Yuki, Kouta Takeda, Hiroyuki Ohno, and Nobuhumi Nakamura. 2017. "Immobilization of Pyrroloquinoline Quinone-Dependent Alcohol Dehydrogenase with a Polyion Complex and Redox Polymer for a Bioanode" Catalysts 7, no. 10: 296. https://doi.org/10.3390/catal7100296

APA Style

Sakurada, Y., Takeda, K., Ohno, H., & Nakamura, N. (2017). Immobilization of Pyrroloquinoline Quinone-Dependent Alcohol Dehydrogenase with a Polyion Complex and Redox Polymer for a Bioanode. Catalysts, 7(10), 296. https://doi.org/10.3390/catal7100296

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Immobilization of Pyrroloquinoline Quinone-Dependent Alcohol Dehydrogenase with a Polyion Complex and Redox Polymer for a Bioanode

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