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Open AccessArticle

Preoperative Typing of Thyroid and Parathyroid Tumors with a Combined Molecular Classifier

1
Department of the Structure and Function of Chromosomes, Institute of Molecular and Cellular Biology, SB RAS, 630090 Novosibirsk, Russia
2
AO Vector-Best, 630117 Novosibirsk, Russia
3
Department of Natural Sciences, Novosibirsk State University, 630090 Novosibirsk, Russia
4
National Center of Clinical Morphological Diagnostics, 192283 Saint Petersburg, Russia
5
Institute of Molecular Pathology and Pathomorphology, 630117 Novosibirsk, Russia
6
Institute of Cytology and Genetics, SB RAS, 630090 Novosibirsk, Russia
7
Department of Endocrinology and Endocrine Surgery of Saint Petersburg State University N.I. Pirogov Clinic of High Medical Technologies, 190103 Saint Petersburg, Russia
*
Author to whom correspondence should be addressed.
Cancers 2021, 13(2), 237; https://doi.org/10.3390/cancers13020237
Received: 24 December 2020 / Revised: 5 January 2021 / Accepted: 6 January 2021 / Published: 11 January 2021
(This article belongs to the Special Issue Biomarkers of Thyroid Cancer)
We previously proposed a new diagnostic algorithm that allows identification and classification of malignancy markers of thyroid tumors in cytological preparations of biopsy material through an analysis of several molecular markers. We previously evaluated the diagnostic characteristics of this algorithm on a sample of category III and IV cytological preparations (Bethesda system, 2017) for the detection of malignant tumors. However, in that study, we did not determine the accuracy of classification. Also, the algorithm did not allow discrimination of parathyroid gland nodules. In the present work, our goal was to include the identification of parathyroid cells in the molecular classifier and to evaluate the performance of our algorithm on the typing of thyroid tumors. We demonstrated that the diagnostic panel including the analysis of microRNA and mRNA expression, the V600E mutation in the BRAF gene, and mitochondrial-to-nuclear DNA ratio enables accurate identification of parathyroid and several types of thyroid carcinomas.
In previous studies, we described a method for detecting and typing malignant tumors of the thyroid gland in fine-needle aspiration biopsy samples via analysis of a molecular marker panel (normalized HMGA2 mRNA level; normalized microRNA-146b, -221, and -375 levels; mitochondrial-to-nuclear DNA ratio; and BRAFV600E mutation) in cytological preparations by quantitative PCR. In the present study, we aimed to estimate the specificity of the typing of different thyroid tumors by the proposed method. Fine-needle aspiration cytological preparations from 278 patients were used. The histological diagnosis was known for each sample. The positive and negative predictive values of the method assessed in this study were, respectively, 100% and 98% for papillary thyroid carcinoma (n = 63), 100% and 100% for medullary thyroid carcinoma (n = 19), 43.5% and 98% for follicular carcinoma (n = 15), and 86% and 100% for Hürthle cell carcinoma (n = 6). Thus, we demonstrate that the diagnostic panel, including the analysis of microRNA expression, mRNA expression, the BRAFV600E mutation, and the mitochondrial-to-nuclear DNA ratio, allows the highly accurate identification of papillary thyroid carcinoma, medullary thyroid carcinoma, and Hürthle cell carcinoma but not malignant follicular tumors (positive predictive value was below 50%). View Full-Text
Keywords: thyroid carcinoma; parathyroid adenoma; preoperative diagnosis; molecular marker; HMGA2; microRNA; mitochondrial DNA thyroid carcinoma; parathyroid adenoma; preoperative diagnosis; molecular marker; HMGA2; microRNA; mitochondrial DNA
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MDPI and ACS Style

Titov, S.E.; Kozorezova, E.S.; Demenkov, P.S.; Veryaskina, Y.A.; Kuznetsova, I.V.; Vorobyev, S.L.; Chernikov, R.A.; Sleptsov, I.V.; Timofeeva, N.I.; Ivanov, M.K. Preoperative Typing of Thyroid and Parathyroid Tumors with a Combined Molecular Classifier. Cancers 2021, 13, 237. https://doi.org/10.3390/cancers13020237

AMA Style

Titov SE, Kozorezova ES, Demenkov PS, Veryaskina YA, Kuznetsova IV, Vorobyev SL, Chernikov RA, Sleptsov IV, Timofeeva NI, Ivanov MK. Preoperative Typing of Thyroid and Parathyroid Tumors with a Combined Molecular Classifier. Cancers. 2021; 13(2):237. https://doi.org/10.3390/cancers13020237

Chicago/Turabian Style

Titov, Sergei E.; Kozorezova, Evgeniya S.; Demenkov, Pavel S.; Veryaskina, Yulia A.; Kuznetsova, Irina V.; Vorobyev, Sergey L.; Chernikov, Roman A.; Sleptsov, Ilya V.; Timofeeva, Nataliya I.; Ivanov, Mikhail K. 2021. "Preoperative Typing of Thyroid and Parathyroid Tumors with a Combined Molecular Classifier" Cancers 13, no. 2: 237. https://doi.org/10.3390/cancers13020237

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