Next Article in Journal
Newly-Discovered Neural Features Expand the Pathobiological Knowledge of Blastic Plasmacytoid Dendritic Cell Neoplasm
Next Article in Special Issue
Current Technologies for RNA-Directed Liquid Diagnostics
Previous Article in Journal
Tumor Microenvironment of Esophageal Cancer
Previous Article in Special Issue
Circulating Long Non-Coding RNAs as Novel Potential Biomarkers for Osteogenic Sarcoma
Article

High Cell-Free DNA Integrity Is Associated with Poor Breast Cancer Survival

1
Institute of Clinical Medicine, Pathology and Forensic Medicine, University of Eastern Finland, FI-70211 Kuopio, Finland
2
Cancer Center, Kuopio University Hospital, FI-70029 Kuopio, Finland
3
Department of Clinical Pathology, Kuopio University Hospital, FI-70029 Kuopio, Finland
4
Multidisciplinary Cancer Research Community (RC Cancer), University of Eastern Finland, FI-70211 Kuopio, Finland
5
Biobank of Eastern Finland, Kuopio University Hospital, FI-70029 Kuopio, Finland
*
Author to whom correspondence should be addressed.
Academic Editors: Nikolas von Bubnoff and Florian Scherer
Cancers 2021, 13(18), 4679; https://doi.org/10.3390/cancers13184679
Received: 20 August 2021 / Revised: 10 September 2021 / Accepted: 15 September 2021 / Published: 18 September 2021
(This article belongs to the Special Issue Liquid Nucleic Acid-Based Biomarkers in Solid Tumors)
A recent point of focus in breast cancer (BC) research has been the utilization of cell-free DNA and its concentration (cfDConc) and integrity (cfDI) as potential biomarkers. Though the association of cfDConc and BC survival is already recognized, studies on the prognostic value of cfDI have had contradictory results. The aim of this study was to investigate the prognostic potential of cfDConc and cfDI in Eastern Finnish BC cases with a non-metastatic disease. While the prognostic value of cfDConc remained non-significant in our analyses, high cfDI was an independent prognostic factor for poor overall survival (OS) and breast cancer-specific survival (BCSS). Inclusion of cfDI in the multivariate logistic regression model improved the predictive performance of the model, thus suggesting that the combined use of traditional tumor features and liquid biopsy could help to discriminate BC patients with poor OS and BCSS more accurately at the time of diagnosis.
Background: A recent point of focus in breast cancer (BC) research has been the utilization of cell-free DNA (cfDNA) and its concentration (cfDConc) and integrity (cfDI) as potential biomarkers. Though the association of cfDConc and poor survival is already recognized, studies on the prognostic value of cfDI have had contradictory results. Here, we provide further evidence to support the use of cfDI as a potential biomarker. Methods: We selected 204 Eastern Finnish BC cases with non-metastatic disease and isolated cfDNA from the serum collected at the time of diagnosis before any treatment was given. The cfDConc and cfDI were measured with a fluorometer and electrophoresis and analyzed with 25 years of survival data. Results: High cfDConc was not an independent prognostic factor in our analyses while high cfDI was found to be an independent prognostic factor for poor OS (p = 0.020, hazard ratio (HR) = 1.57, 95% confidence interval (CI) 1.07–2.29, Cox) and BCSS (p = 0.006, HR = 1.93, 95% CI 1.21–3.08)). Inclusion of cfDI in the multivariate logistic regression model improved the predictive performance. Conclusions: Our results show high cfDI is an independent prognostic factor for poor OS and BCSS and improves the predictive performance of logistic regression models, thus supporting its prognostic potential. View Full-Text
Keywords: liquid biopsy; biomarker; DNA fragmentation; diagnostics liquid biopsy; biomarker; DNA fragmentation; diagnostics
Show Figures

Figure 1

MDPI and ACS Style

Lamminaho, M.; Kujala, J.; Peltonen, H.; Tengström, M.; Kosma, V.-M.; Mannermaa, A. High Cell-Free DNA Integrity Is Associated with Poor Breast Cancer Survival. Cancers 2021, 13, 4679. https://doi.org/10.3390/cancers13184679

AMA Style

Lamminaho M, Kujala J, Peltonen H, Tengström M, Kosma V-M, Mannermaa A. High Cell-Free DNA Integrity Is Associated with Poor Breast Cancer Survival. Cancers. 2021; 13(18):4679. https://doi.org/10.3390/cancers13184679

Chicago/Turabian Style

Lamminaho, Maria, Jouni Kujala, Hanna Peltonen, Maria Tengström, Veli-Matti Kosma, and Arto Mannermaa. 2021. "High Cell-Free DNA Integrity Is Associated with Poor Breast Cancer Survival" Cancers 13, no. 18: 4679. https://doi.org/10.3390/cancers13184679

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop