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EV-Associated miRNAs from Peritoneal Lavage are a Source of Biomarkers in Endometrial Cancer

1
Department of Pathology and Molecular Genetics/Oncologic Pathology Group, Biomedical Research Institute of Lleida (IRBLleida), University of Lleida, CIBERONC, 25198 Lleida, Spain
2
Department of Pathology, University Hospital of Bellvitge, Bellvitge Biomedical Research Institute (IDIBELL), 08908 L’Hospitalet de Llobregat, Spain
3
Biomedical Research Group in Gynecology, Vall Hebron Research Institute (VHIR), CIBERONC, 08035 Barcelona, Spain
4
Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Spain
5
Centre for Genomic Regulation (CRG), The Barcelona Institute or Science and Technology, Dr. Aiguader 88, 08003 Barcelona, Spain
6
Exosomes Laboratory and Metabolomics Platform. CIC bioGUNE, CIBEREHD Bizkaia Technology Park, 48160 Derio, Spain
7
Pleural Medicine Unit, Arnau de Vilanova University Hospital, Cancer Biomarker Group, IRBLleida, 25198 Lleida, Spain
8
Gynecological Oncology Department, Vall Hebron University Hospital, CIBERONC, 08035 Barcelona, Spain
9
IKERBASQUE, Basque Foundation for Science, 48011 Bilbao, Spain
10
University Pompeu Fabra, 08002 Barcelona, Spain
*
Authors to whom correspondence should be addressed.
Cancers 2019, 11(6), 839; https://doi.org/10.3390/cancers11060839
Received: 29 April 2019 / Revised: 13 June 2019 / Accepted: 14 June 2019 / Published: 18 June 2019
(This article belongs to the Special Issue New Insights into Breast and Endometrial Cancer)
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Abstract

Endometrial cancer (EC) is the sixth most common cancer in women worldwide and is responsible for more than 89,000 deaths every year. Mortality is associated with presence of poor prognostic factors at diagnosis, i.e., diagnosis at an advanced stage, with a high grade and/or an aggressive histology. Development of novel approaches that would permit us to improve the clinical management of EC patients is an unmet need. In this study, we investigate a novel approach to identify highly sensitive and specific biomarkers of EC using extracellular vesicles (EVs) isolated from the peritoneal lavage of EC patients. EVs of peritoneal lavages of 25 EC patients were isolated and their miRNA content was compared with miRNAs of EVs isolated from the ascitic fluid of 25 control patients. Expression of the EV-associated miRNAs was measured using the Taqman OpenArray technology that allowed us to detect 371 miRNAs. The analysis showed that 114 miRNAs were significantly dysregulated in EC patients, among which eight miRNAs, miRNA-383-5p, miRNA-10b-5p, miRNA-34c-3p, miRNA-449b-5p, miRNA-34c-5p, miRNA-200b-3p, miRNA-2110, and miRNA-34b-3p, demonstrated a classification performance at area under the receiver operating characteristic curve (AUC) values above 0.9. This finding opens an avenue for the use of EV-associated miRNAs of peritoneal lavages as an untapped source of biomarkers for EC. View Full-Text
Keywords: endometrial cancer; uterine cancer; exosomes; biomarkers; miRNAs; ascitic fluid; peritoneal lavage; liquid biopsy; extracellular vesicles; microRNAs endometrial cancer; uterine cancer; exosomes; biomarkers; miRNAs; ascitic fluid; peritoneal lavage; liquid biopsy; extracellular vesicles; microRNAs
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Roman-Canal, B.; Moiola, C.P.; Gatius, S.; Bonnin, S.; Ruiz-Miró, M.; González, E.; González-Tallada, X.; Llordella, I.; Hernández, I.; Porcel, J.M.; Gil-Moreno, A.; Falcón-Pérez, J.M.; Ponomarenko, J.; Matias-Guiu, X.; Colas, E. EV-Associated miRNAs from Peritoneal Lavage are a Source of Biomarkers in Endometrial Cancer. Cancers 2019, 11, 839.

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