Loss of Cyclin-Dependent Kinase Inhibitor Alters Oncolytic Adenovirus Replication and Promotes More Efficient Virus Production

Höti, Naseruddin; Johnson, Tamara Jane; Chowdhury, Wasim H.; Rodriguez, Ronald

doi:10.3390/cancers10060202

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Loss of Cyclin-Dependent Kinase Inhibitor Alters Oncolytic Adenovirus Replication and Promotes More Efficient Virus Production

^1,†,

^2,†,

and

^3,*

Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA

James Brady Urological Institute, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA

Department of Urology, University of Texas Health Science Center San Antonio, San Antonio, TX 78229, USA

Author to whom correspondence should be addressed.

^†

These authors contributed equally to the work.

Cancers 2018, 10(6), 202; https://doi.org/10.3390/cancers10060202

Received: 10 May 2018 / Revised: 6 June 2018 / Accepted: 11 June 2018 / Published: 15 June 2018

(This article belongs to the Special Issue Oncolytic Virotherapy)

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Abstract

We elucidate the role of p21/Waf-1, a cyclin-dependent kinase inhibitor, on the oncolytic infection and replication cycle of adenovirus by studying both mRNA and adenoviral proteins expression. We found that infection in the absence of p21 causes a significant increase in adenoviral genomes and late gene expression. Similarly, the oncolytic adenoviral infected p21^−/− cells have earlier formation of replication foci and robust replication kinetics that were not observed in the wild type p21/Waf-1 intact cells. These findings suggest a culmination that the presence of intact p21 in host cells causes defects in the oncolytic viral life cycle which results in the production of immature and noninfectious particles. View Full-Text

Keywords: adenovirus replication; p21; oncolytic adenovirus

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MDPI and ACS Style

Höti, N.; Johnson, T.J.; Chowdhury, W.H.; Rodriguez, R. Loss of Cyclin-Dependent Kinase Inhibitor Alters Oncolytic Adenovirus Replication and Promotes More Efficient Virus Production. Cancers 2018, 10, 202. https://doi.org/10.3390/cancers10060202

AMA Style

Höti N, Johnson TJ, Chowdhury WH, Rodriguez R. Loss of Cyclin-Dependent Kinase Inhibitor Alters Oncolytic Adenovirus Replication and Promotes More Efficient Virus Production. Cancers. 2018; 10(6):202. https://doi.org/10.3390/cancers10060202

Chicago/Turabian Style

Höti, Naseruddin, Tamara J. Johnson, Wasim H. Chowdhury, and Ronald Rodriguez. 2018. "Loss of Cyclin-Dependent Kinase Inhibitor Alters Oncolytic Adenovirus Replication and Promotes More Efficient Virus Production" Cancers 10, no. 6: 202. https://doi.org/10.3390/cancers10060202

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Loss of Cyclin-Dependent Kinase Inhibitor Alters Oncolytic Adenovirus Replication and Promotes More Efficient Virus Production

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