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Micromachines 2017, 8(11), 335; https://doi.org/10.3390/mi8110335

A Microfluidic Device for Culturing an Encapsulated Ovarian Follicle

1
Department of Biomedical Engineering, Dalian University of Technology, Dalian 116024, China
2
Dalian Institute of Maternal and Child Health Care, Dalian 116024, China
3
School of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian 116024, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 4 October 2017 / Revised: 16 November 2017 / Accepted: 17 November 2017 / Published: 20 November 2017
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Abstract

Microfluidic chips have been proved effective in mimicking different organs of human body. Simulating human ovarian follicles by microfluidic device will be useful in exploring the mechanism of folliculogenesis and related diseases. In this paper, a microfluidic chip was designed to culture a single human pre-antral follicle. Ovarian follicles were first encapsulated in 3D calcium alginate hydrogel beads and then cultured on chip and in dish under same conditions. The diameters of cultured ovarian follicles were measured, and the same amount of medium was collected from microfluidic device or dish per two days for measuring the estradiol and androgen concentrations. The results confirmed the successful growth of ovarian follicles on chip with their hormonal trends and diameters increase, which were similar to ovarian follicles cultured in dish. It is concluded that this microfluidic chip can be used to culture a single human ovarian follicle, which provides a useful tool to explore the hormonal changes and their interactions during folliculogenesis. View Full-Text
Keywords: ovarian follicles; microfluidics; organ on a chip ovarian follicles; microfluidics; organ on a chip
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Aziz, A.U.R.; Fu, M.; Deng, J.; Geng, C.; Luo, Y.; Lin, B.; Yu, X.; Liu, B. A Microfluidic Device for Culturing an Encapsulated Ovarian Follicle. Micromachines 2017, 8, 335.

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