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Open AccessArticle

Selective Retrieval of Individual Cells from Microfluidic Arrays Combining Dielectrophoretic Force and Directed Hydrodynamic Flow

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Laboratory of Life Sciences Electronics, École Polytechnique Fédérale de Lausanne, 1015 Lausanne, CH, Switzerland
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Laboratory of Systems Biology and Genetics, École Polytechnique Fédérale de Lausanne, 1015 Lausanne, CH, Switzerland
*
Author to whom correspondence should be addressed.
Micromachines 2020, 11(3), 322; https://doi.org/10.3390/mi11030322
Received: 14 February 2020 / Revised: 12 March 2020 / Accepted: 19 March 2020 / Published: 20 March 2020
(This article belongs to the Special Issue Micromachines for Dielectrophoresis)
Hydrodynamic-based microfluidic platforms enable single-cell arraying and analysis over time. Despite the advantages of established microfluidic systems, long-term analysis and proliferation of cells selected in such devices require off-chip recovery of cells as well as an investigation of on-chip analysis on cell phenotype, requirements still largely unmet. Here, we introduce a device for single-cell isolation, selective retrieval and off-chip recovery. To this end, singularly addressable three-dimensional electrodes are embedded within a microfluidic channel, allowing the selective release of single cells from their trapping site through application of a negative dielectrophoretic (DEP) force. Selective capture and release are carried out in standard culture medium and cells can be subsequently mitigated towards a recovery well using micro-engineered hybrid SU-8/PDMS pneumatic valves. Importantly, transcriptional analysis of recovered cells revealed only marginal alteration of their molecular profile upon DEP application, underscored by minor transcriptional changes induced upon injection into the microfluidic device. Therefore, the established microfluidic system combining targeted DEP manipulation with downstream hydrodynamic coordination of single cells provides a powerful means to handle and manipulate individual cells within one device. View Full-Text
Keywords: single-cell microfluidics; single-cell recovery; single-cell array; hydrodynamic trapping; electrokinetics; tridimensional electrodes; dielectrophoresis (DEP); mRNA sequencing; Drop-seq single-cell microfluidics; single-cell recovery; single-cell array; hydrodynamic trapping; electrokinetics; tridimensional electrodes; dielectrophoresis (DEP); mRNA sequencing; Drop-seq
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Thiriet, P.-E.; Pezoldt, J.; Gambardella, G.; Keim, K.; Deplancke, B.; Guiducci, C. Selective Retrieval of Individual Cells from Microfluidic Arrays Combining Dielectrophoretic Force and Directed Hydrodynamic Flow. Micromachines 2020, 11, 322.

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