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Core-Shell Beads Made by Composite Liquid Marble Technology as A Versatile Microreactor for Polymerase Chain Reaction

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Queensland Micro- and Nanotechnology Centre, Nathan Campus, Griffith University, 170 Kessels Road, Brisbane, QLD 4111, Australia
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School of Environment and Science, Nathan Campus, Griffith University, 170 Kessels Road, Brisbane, QLD 4111, Australia
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Department of Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan
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Frontier Research Institute for Interdisciplinary Science, Tohoku University, 6-3 Aramaki aza Aoba-ku, Sendai, Miyagi 980-8578, Japan
*
Author to whom correspondence should be addressed.
Micromachines 2020, 11(3), 242; https://doi.org/10.3390/mi11030242
Received: 28 January 2020 / Revised: 21 February 2020 / Accepted: 25 February 2020 / Published: 26 February 2020
(This article belongs to the Section B:Biology and Biomedicine)
Over the last three decades, the protocols and procedures of the DNA amplification technique, polymerase chain reaction (PCR), have been optimized and well developed. However, there have been no significant innovations in processes for sample dispersion for PCR that have reduced the amount of single-use or unrecyclable plastic waste produced. To address the issue of plastic waste, this paper reports the synthesis and successful use of a core-shell bead microreactor using photopolymerization of a composite liquid marble as a dispersion process. This platform uses the core-shell bead as a simple and effective sample dispersion medium that significantly reduces plastic waste generated compared to conventional PCR processes. Other improvements over conventional PCR processes of the novel dispersion platform include increasing the throughput capability, enhancing the performance and portability of the thermal cycler, and allowing for the contamination-free storage of samples after thermal cycling. View Full-Text
Keywords: composite liquid marble; core-shell bead; polymerase chain reaction (PCR) composite liquid marble; core-shell bead; polymerase chain reaction (PCR)
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Sreejith, K.R.; Gorgannezhad, L.; Jin, J.; Ooi, C.H.; Takei, T.; Hayase, G.; Stratton, H.; Lamb, K.; Shiddiky, M.; Dao, D.V.; Nguyen, N.-T. Core-Shell Beads Made by Composite Liquid Marble Technology as A Versatile Microreactor for Polymerase Chain Reaction. Micromachines 2020, 11, 242.

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