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Adsorption and Absorption of Collagen Peptides to Polydimethlysiloxane and Its Influence on Platelet Adhesion Flow Assays

Chemical and Biological Engineering Department, Colorado School of Mines, Golden, CO 80401, USA
Departments of Bioengineering and Pediatrics, University of Colorado Denver|Anschutz Medical Campus, Aurora, CO 80045, USA
Author to whom correspondence should be addressed.
Micromachines 2020, 11(1), 62;
Received: 15 October 2019 / Revised: 30 December 2019 / Accepted: 2 January 2020 / Published: 5 January 2020
(This article belongs to the Special Issue Blood-on-a-Chip)
Collagen peptides are an alternative to animal derived collagens for platelet function studies under flow. The purpose of this study was to examine the use of collagen peptides in polydimethylsiloxane (PDMS) devices. Three collagen peptides with amino acid sequences and structures that capture von Willebrand factor and bind it with the platelet receptors integrin α2β1 and glycoprotein VI were patterned on glass, silicon, and PDMS. Each of these surfaces was also functionalized with tridecafluoro-1,1,2,2-tetrahydrooctyltrichlorosilane (FOTS). Surfaces were characterized by their ability to support platelet adhesion, topology by atomic force microscopy, contact angle, and peptides absorption. PDMS readily absorbs collagen peptides, depleting them from solution, thus reducing their adsorption to glass and silicon substrates when used for micropatterning. Treatment of PDMS with FOTS, but not bovine serum albumin or poloxamer 407, inhibits collagen peptide absorption and supports adsorption and platelet adhesion at venous and arterial shear rates. Similarly, FOTS treatment of glass or silicon supports collagen peptide adsorption even in the presence of untreated PDMS. In conclusion, PDMS acts as an absorptive sink for collagen peptides, rendering a non-adhesive surface for platelet adhesion and competing for peptides when used for micropatterning. The absorption of collagen peptides can be overcome by functionalization of PDMS with a fluorinated alkyl silane, thus allowing its use as a material for micropatterning or as a surface for platelet adhesion flow assays. View Full-Text
Keywords: platelets; collagen peptides; cell adhesion; polydimethylsiloxane platelets; collagen peptides; cell adhesion; polydimethylsiloxane
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Sorrells, M.G.; Neeves, K.B. Adsorption and Absorption of Collagen Peptides to Polydimethlysiloxane and Its Influence on Platelet Adhesion Flow Assays. Micromachines 2020, 11, 62.

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