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Silver Doped Mesoporous Silica Nanoparticles Based Electrochemical Enzyme-Less Sensor for Determination of H2O2 Released from Live Cells

1
Department of Preventative Medicine, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Medical School of Ningbo University, Ningbo 315211, China
2
Institute of Pharmaceutical Analysis and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy, Jinan University, Guangzhou 510632, China
3
School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
*
Authors to whom correspondence should be addressed.
Micromachines 2019, 10(4), 268; https://doi.org/10.3390/mi10040268
Received: 21 March 2019 / Revised: 9 April 2019 / Accepted: 9 April 2019 / Published: 21 April 2019
(This article belongs to the Special Issue Biosensors for Pathogen Detection)
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Abstract

In this study, a silver doped mesoporous silica nanoparticles-based enzyme-less electrochemical sensor for the determination of hydrogen peroxide (H2O2) released from live cells was constructed for the first time. The presented electrochemical sensor exhibited fast response (2 s) towards the reduction of H2O2 concentration variation at an optimized potential of −0.5 V with high selectivity over biological interferents such as uric acid, ascorbic acid, and glucose. In addition, a wide linear range (4 μM to 10 mM) with a low detection limit (LOD) of 3 μM was obtained. Furthermore, the Ag-mSiO2 nanoparticles/glass carbon electrode (Ag-mSiO2 NPs/GCE) based enzyme-less sensor showed good electrocatalytic performance, as well as good reproducibility, and long-term stability, which provided a successful way to in situ determine H2O2 released from live cells. It may also be promising to monitor the effect of reactive oxygen species (ROS) production in bacteria against oxidants and antibiotics. View Full-Text
Keywords: silver-mesoporous silica nanoparticles; hydrogen peroxide; electrochemical sensor; live cells silver-mesoporous silica nanoparticles; hydrogen peroxide; electrochemical sensor; live cells
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Yang, D.; Ni, N.; Cao, L.; Song, X.; Alhamoud, Y.; Yu, G.; Zhao, J.; Zhou, H. Silver Doped Mesoporous Silica Nanoparticles Based Electrochemical Enzyme-Less Sensor for Determination of H2O2 Released from Live Cells. Micromachines 2019, 10, 268.

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