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Toxins 2017, 9(11), 374; https://doi.org/10.3390/toxins9110374

Impact of a Single Oral Acute Dose of Aflatoxin B1 on Liver Function/Cytokines and the Lymphoproliferative Response in C57Bl/6 Mice

1
Department of Pathological Sciences, State University of Londrina, P.O. Box 10.011, Londrina 86057-970, Paraná, Brazil
2
Department of Food Science and Technology, State University of Londrina, P.O. Box 10.011, Londrina 86057-970, Paraná, Brazil
3
Department of Preventive Veterinary Medicine, State University of Londrina, P.O. Box 10.011, Londrina 86051-970, Paraná, Brazil
4
Food Hygiene Laboratory, Faculty of Agriculture, Kagawa University, Miki-cho 761-0795, Kagawa, Japan
5
Department of Veterinary Biomedicine, University of Montreal, 3200 Rue Sicotte, St-Hyacinthe, QC J2S 2M2, Canada
*
Authors to whom correspondence should be addressed.
Academic Editor: Chiara Dall’Asta
Received: 16 October 2017 / Revised: 15 November 2017 / Accepted: 15 November 2017 / Published: 17 November 2017
(This article belongs to the Special Issue Impact of Human Metabolism on the Toxicological Effects of Mycotoxins)
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Abstract

Aflatoxin B1 (AFB1), a mycotoxin found in food and feed, exerts harmful effects on humans and animals. The liver is the earliest target of AFB1, and its effects have been evaluated in animal models exposed to acute or chronic doses. Considering the possibility of sporadic ingestion of AFB1-contaminated food, this study investigated the impact of a single oral dose of AFB1 on liver function/cytokines and the lymphoproliferative response in mice. C57BL/6 mice were treated with a single oral AFB1 dose (44, 442 or 663 μg AFB1/kg of body weight) on the first day. Liver function (ALT, γ-GT, and total protein), cytokines (IL-4, IFN-γ, and IL-17), histopathology, and the spleen lymphoproliferative response to mitogens were evaluated on the 5th day. Although AFB1 did not produce any significant changes in the biochemical parameters, 663 μg AFB1/kg-induced hepatic upregulation of IL-4 and IFN-γ, along with liver tissue injury and suppression of the lymphoproliferative response to ConA (p < 0.05). In conclusion, a single oral dose of AFB1 exposure can induce liver tissue lesions, liver cytokine modulation, and immune suppression in C57BL/6 mice. View Full-Text
Keywords: cytokines; inflammatory response; immunosuppression; mycotoxin cytokines; inflammatory response; immunosuppression; mycotoxin
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Ishikawa, A.T.; Hirooka, E.Y.; Alvares e Silva, P.L.; Bracarense, A.P.F.R.L.; Flaiban, K.K.M.C.; Akagi, C.Y.; Kawamura, O.; Costa, M.C.; Itano, E.N. Impact of a Single Oral Acute Dose of Aflatoxin B1 on Liver Function/Cytokines and the Lymphoproliferative Response in C57Bl/6 Mice. Toxins 2017, 9, 374.

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