Next Article in Journal
Rural Subsistence Maize Farming in South Africa: Risk Assessment and Intervention models for Reduction of Exposure to Fumonisin Mycotoxins
Next Article in Special Issue
Effects of Destruxin A on Silkworm’s Immunophilins
Previous Article in Journal
The Role of Streptococcal and Staphylococcal Exotoxins and Proteases in Human Necrotizing Soft Tissue Infections
Previous Article in Special Issue
The Relationship Analysis on Corn Stalk Rot and Ear Rot According to Fusarium Species and Fumonisin Contamination in Kernels
Article Menu

Export Article

Open AccessArticle

Effects of Dietary Zearalenone on Oxidative Stress, Cell Apoptosis, and Tight Junction in the Intestine of Juvenile Grass Carp (Ctenopharyngodon idella)

1,†, 1,2,3,†, 1,2,4, 1,2,4, 1,2,5, 1, 6, 7, 7 and 1,2,3,*
1
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China
2
Fish Nutrition and safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China
3
Key Laboratory for Animal Disease-Resistance Nutrition, Chengdu 611130, China
4
Key Laboratory of Animal Disease-Resistant Nutrition, Ministry of Education, Chengdu 611130, China
5
Key Laboratory of Animal Disease-Resistant Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Chengdu 611130, China
6
Tongwei Research Institute, Tongwei Co., Ltd., Chengdu 600438, China
7
Animal Nutrition Institute, Sichuan Academy of Animal Science, Sichuan Animtech Feed. Co., Ltd., Chengdu 610066, China
*
Author to whom correspondence should be addressed.
These two authors contributed to this work equally.
Toxins 2019, 11(6), 333; https://doi.org/10.3390/toxins11060333
Received: 20 April 2019 / Revised: 28 May 2019 / Accepted: 6 June 2019 / Published: 12 June 2019
(This article belongs to the Special Issue Fungal Infestations in Humans, Animals, Crops)
  |  
PDF [4870 KB, uploaded 12 June 2019]
  |  

Abstract

Zearalenone (ZEA) is a prevalent mycotoxin with high toxicity in animals. In order to study its effect on juvenile grass carp (Ctenopharyngodon idella), six diets supplemented with different levels of ZEA (0, 535, 1041, 1548, 2002, and 2507 μg/kg diet) for 10 weeks were studied to assess its toxicity on intestinal structural integrity and potential mechanisms of action. Our report firstly proved that ZEA led to growth retardation and body deformity, and impaired the intestinal structural integrity of juvenile grass carp, as revealed by the following findings: (1) ZEA accumulated in the intestine and caused histopathological lesions; (2) ZEA resulted in oxidative injury, apoptosis, and breached tight junctions in the fish intestine, which were probably associated with Nuclear factor-erythroid 2-related factor 2 (Nrf2), p38 mitogen activated protein kinases (p38MAPK), and myosin light chain kinase (MLCK) signaling pathways, respectively. ZEA had no influence on the antioxidant gene levels of Kelch-like ECH associating protein 1 (Keap1)b (rather than Keap1a), glutathione-S-transferase (GST)P1, GSTP2 (not in the distal intestine (DI)), tight junctions occludin, claudin-c (not in the proximal intestine (PI)), or claudin-3c (not in the mid intestine (MI) or DI). View Full-Text
Keywords: zearalenone; intestine; oxidative injury; apoptosis; tight junctions zearalenone; intestine; oxidative injury; apoptosis; tight junctions
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Wang, Y.-L.; Zhou, X.-Q.; Jiang, W.-D.; Wu, P.; Liu, Y.; Jiang, J.; Wang, S.-W.; Kuang, S.-Y.; Tang, L.; Feng, L. Effects of Dietary Zearalenone on Oxidative Stress, Cell Apoptosis, and Tight Junction in the Intestine of Juvenile Grass Carp (Ctenopharyngodon idella). Toxins 2019, 11, 333.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top