Effects of High-Dose Vitamin D Supplementation on Placental Vitamin D Metabolism and Neonatal Vitamin D Status
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Population
2.2. Placental Collection
2.3. Umbilical Cord Blood
2.4. 25(OH)D Analysis
2.5. VDBP Analysis
2.6. RNA Extraction
2.7. qPCR
2.8. Statistics and Descriptive Analysis
3. Results
3.1. Placental Expression of vitD-Related Genes
3.1.1. Correlation to the Third-Trimester Maternal Serum 25(OH)D
3.1.2. The Effect of Pre-Pregnancy BMI
3.1.3. Impact of Early vitD Status
3.1.4. Delivery Mode and Offspring Sex
3.2. Maternal VDBP during Pregnancy
3.3. Neonatal vitD Status
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Primer |
---|---|
CUBN | |
Forward | 5′-CTGGACGGCCATTACTCACA-3′ |
Reverse | 5′-CTGAGACTGGAAGACGGCAG-3′ |
LRP2 | |
Forward | 5′-GCCCTTTCGCTGTCCTAGTT-3′ |
Reverse | 5′-AGGGCTCTTGAACACACTCG-3′ |
VDR | |
Forward | 5′-GCCTGACCCTGGAGACTTTG-3′ |
Reverse | 5′-GGGCAGGTGAATAGTGCCTT-3′ |
HPR1T | |
Forward | 5′-CCTGGCGTCGTGATTAGTGA-3′ |
Reverse | 5′-GAGCACACAGAGGGCTACAA-3′ |
CycA | |
Forward | 5′-GCCGAGGAAAACCGTGTACTA-3′ |
Reverse | 5′-ACCCTGACACATAAACCCTGG-3′ |
Probe | |
CYP24A1 | HS00167999_m1 |
CYP27B1 | HS01096154_m1 |
HPRT1 | HS02800695_m1 |
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Vestergaard, A.L.; Andersen, M.K.; Andersen, H.H.; Bossow, K.A.; Bor, P.; Larsen, A. Effects of High-Dose Vitamin D Supplementation on Placental Vitamin D Metabolism and Neonatal Vitamin D Status. Nutrients 2024, 16, 2145. https://doi.org/10.3390/nu16132145
Vestergaard AL, Andersen MK, Andersen HH, Bossow KA, Bor P, Larsen A. Effects of High-Dose Vitamin D Supplementation on Placental Vitamin D Metabolism and Neonatal Vitamin D Status. Nutrients. 2024; 16(13):2145. https://doi.org/10.3390/nu16132145
Chicago/Turabian StyleVestergaard, Anna Louise, Matilde Kanstrup Andersen, Helena Hørdum Andersen, Krista Agathe Bossow, Pinar Bor, and Agnete Larsen. 2024. "Effects of High-Dose Vitamin D Supplementation on Placental Vitamin D Metabolism and Neonatal Vitamin D Status" Nutrients 16, no. 13: 2145. https://doi.org/10.3390/nu16132145