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Article

Effect of Microbial Status on Hepatic Odd-Chain Fatty Acids Is Diet-Dependent

1
Department Physiology of Energy Metabolism, German Institute of Human Nutrition Potsdam-Rehbruecke (DIfE), 14558 Nuthetal, Germany
2
Research Group Intestinal Microbiology, German Institute of Human Nutrition Potsdam-Rehbruecke (DIfE), 14558 Nuthetal, Germany
3
Department Molecular Epidemiology, German Institute of Human Nutrition Potsdam-Rehbruecke (DIfE), 14558 Nuthetal, Germany
4
Institute of Nutritional Science, University of Potsdam, 14469 Potsdam, Germany
*
Author to whom correspondence should be addressed.
Academic Editors: Julio Galvez and Alba Rodriguez-Nogales
Nutrients 2021, 13(5), 1546; https://doi.org/10.3390/nu13051546
Received: 13 April 2021 / Revised: 28 April 2021 / Accepted: 30 April 2021 / Published: 4 May 2021
(This article belongs to the Special Issue Nutrition and Metabolic Syndrome Management)
Odd-chain fatty acids (OCFA) are inversely associated with type-2-diabetes in epidemiological studies. They are considered as a biomarker for dairy intake because fermentation in ruminants yields high amounts of propionate, which is used as the primer for lipogenesis. Recently, we demonstrated endogenous OCFA synthesis from propionate in humans and mice, but how this is affected by microbial colonization is still unexplored. Here, we investigated the effect of increasing microbiota complexity on hepatic lipid metabolism and OCFA levels in different dietary settings. Germ-free (GF), gnotobiotic (SIH, simplified human microbiota) or conventional (CONV) C3H/HeOuJ-mice were fed a CHOW or high-fat diet with inulin (HFI) to induce microbial fermentation. We found that hepatic lipogenesis was increased with increasing microbiota complexity, independently of diet. In contrast, OCFA formation was affected by diet as well as microbiota. On CHOW, hepatic OCFA and intestinal gluconeogenesis decreased with increasing microbiota complexity (GF > SIH > CONV), while cecal propionate showed a negative correlation with hepatic OCFA. On HFI, OCFA levels were highest in SIH and positively correlated with cecal propionate. The propionate content in the CHOW diet was 10 times higher than that of HFI. We conclude that bacterial propionate production affects hepatic OCFA formation, unless this effect is masked by dietary propionate intake. View Full-Text
Keywords: pentadecanoic acid (C15:0); heptadecanoic acid (C17:0); type-2-diabetes; fatty acid synthesis; acetate; propionate; probiotics; gut microbiota; prebiotics; inulin pentadecanoic acid (C15:0); heptadecanoic acid (C17:0); type-2-diabetes; fatty acid synthesis; acetate; propionate; probiotics; gut microbiota; prebiotics; inulin
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MDPI and ACS Style

Weitkunat, K.; Bishop, C.A.; Wittmüss, M.; Machate, T.; Schifelbein, T.; Schulze, M.B.; Klaus, S. Effect of Microbial Status on Hepatic Odd-Chain Fatty Acids Is Diet-Dependent. Nutrients 2021, 13, 1546. https://doi.org/10.3390/nu13051546

AMA Style

Weitkunat K, Bishop CA, Wittmüss M, Machate T, Schifelbein T, Schulze MB, Klaus S. Effect of Microbial Status on Hepatic Odd-Chain Fatty Acids Is Diet-Dependent. Nutrients. 2021; 13(5):1546. https://doi.org/10.3390/nu13051546

Chicago/Turabian Style

Weitkunat, Karolin, Christopher A. Bishop, Maria Wittmüss, Tina Machate, Tina Schifelbein, Matthias B. Schulze, and Susanne Klaus. 2021. "Effect of Microbial Status on Hepatic Odd-Chain Fatty Acids Is Diet-Dependent" Nutrients 13, no. 5: 1546. https://doi.org/10.3390/nu13051546

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