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International Journal of Plant Biology is published by MDPI from Volume 13 Issue 1 (2022). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with PAGEPress.
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Article

In Vitro Culture as an Aid to Conservation of Indigenous Ferns: Diplazium Proliferum

by
Zubeir M. Golamaully
1,
Vishwakalyan Bhoyroo
1,2,*,
Nadeem Nazurally
1 and
Vineshwar Gopal
3
1
Faculty of Agriculture, University of Mauritius, Reduit 80837, Mauritius
2
Department of Agricultural and Food Sciences, Faculty of Agriculture, University of Mauritius, Reduit 80837, Mauritius
3
National Parks and Conservation Services, Reduit, Mauritius
*
Author to whom correspondence should be addressed.
Int. J. Plant Biol. 2015, 6(1), 6020; https://doi.org/10.4081/pb.2015.6020
Submission received: 16 May 2015 / Revised: 24 July 2015 / Accepted: 24 July 2015 / Published: 20 November 2015

Abstract

With the ever growing population and economic needs of Mauritius, the flora of Mauritius has never been in more danger and one group of vascular plants is even more in peril; ferns. Diplazium proliferum is indigenous to the Mascarene region and is considered as a rare species in Mauritius. The need to develop a tested in vitro propagation protocol is a must to protect the biodiversity of Mauritius. This experiment was geared towards the establishment of a proper sterilization technique and the effect of 6-benzylaminopurine (BAP) and light on in vitro culture of this fern. Sterilization with 0.05% Mercuric chloride was effective to eliminate fungal contamination and allow germination of spores. Culture media supplemented with BAP did not significantly increase growth rate of both gametophytes and sporophytes of D. proliferum. Present results suggest efficient sterilization methods to be a crucial stage for successful in vitro regeneration of ferns. The established protocol will be used as an optimized baseline protocol for the propagation of other indigenous ferns.
Keywords: ferns; in vitro culture; Diplazium proliferum; conservation ferns; in vitro culture; Diplazium proliferum; conservation

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MDPI and ACS Style

Golamaully, Z.M.; Bhoyroo, V.; Nazurally, N.; Gopal, V. In Vitro Culture as an Aid to Conservation of Indigenous Ferns: Diplazium Proliferum. Int. J. Plant Biol. 2015, 6, 6020. https://doi.org/10.4081/pb.2015.6020

AMA Style

Golamaully ZM, Bhoyroo V, Nazurally N, Gopal V. In Vitro Culture as an Aid to Conservation of Indigenous Ferns: Diplazium Proliferum. International Journal of Plant Biology. 2015; 6(1):6020. https://doi.org/10.4081/pb.2015.6020

Chicago/Turabian Style

Golamaully, Zubeir M., Vishwakalyan Bhoyroo, Nadeem Nazurally, and Vineshwar Gopal. 2015. "In Vitro Culture as an Aid to Conservation of Indigenous Ferns: Diplazium Proliferum" International Journal of Plant Biology 6, no. 1: 6020. https://doi.org/10.4081/pb.2015.6020

APA Style

Golamaully, Z. M., Bhoyroo, V., Nazurally, N., & Gopal, V. (2015). In Vitro Culture as an Aid to Conservation of Indigenous Ferns: Diplazium Proliferum. International Journal of Plant Biology, 6(1), 6020. https://doi.org/10.4081/pb.2015.6020

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