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Microbiology Research
Volume 3
Issue 1
10.4081/mr.2012.e9
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Article

In Vitro Antimicrobial Activity of Salvadora persica Extract on Helicobacter pylori Strains Isolated from Duodenal Ulcer Biopsies

by
Ehsan Mirkamandar
1,
Mohammad Reza Shakibaie
2,*,
Saeed Adeli
2,
Mitra Mehrabani
3,
Mohammad Mehdi Hayatbakhsh
4 and
Saeed Esmailian
1
1
Student Research Committee, Iran
2
Department of Microbiology and Environmental Health Research Center, Iran
3
Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran
4
Gastroenterology Division, Department of Internal Medicine, Afzalipoor Hospital, Kerman, Iran
*
Author to whom correspondence should be addressed.
Microbiol. Res. 2012, 3(1), e9; https://doi.org/10.4081/mr.2012.e9
Submission received: 12 October 2011 / Revised: 8 January 2012 / Accepted: 8 February 2012 / Published: 3 April 2012

Abstract

The aim of this study was to evaluate the in vitro antimicrobial activity of a methanolic extract of Salvadora persica solution on Helicobacter pylori isolated from duodenal ulcer. Over 22 strains of H. pylori were isolated from duodenal ulcer from August 2010 to June 2011. The S. persica stem was purchased from a local herb market and finely powdered. Extraction was performed with 60% methanol using a soxhlet extractor for 48 h until the solvent turned colorless while being incubated in an oven at 40 °C for 48 h till dried. Dry powder was used to determine antimicrobial activity by the agar ditch method. Minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the extract were determined by the agar dilution method. At concentrations of 10, 100, 200, 500 µg/mL, no zone of inhibition around the ditches was observed while a clear zone of inhibition (12 mm) was detected at 1000 µg/mL concentration for all the isolates. The best antimicrobial activity was observed at MIC 1000 µg/mL (P ≤ 0.05). Furthermore, 10 out of 22 isolates were inhibited at 750 µg/mL of the extract. The MBC results showed that at a concentration of 1000 µg/mL all cells were dead while at a concentration of 750 µg/mL of S. persica a few H. pylori cells were still able to form colonies on Brucella agar supplemented with sheep red blood cells and antibiotics. From the above results it can be concluded that high concentration of S.persica could inhibit the growth of H. pylori and MIC and MBC were similar at that concentration.
Keywords: Helicobacter pylori; Salvadora persica; extraction; minimum inhibitory concentration; minimal bactericidal concentration Helicobacter pylori; Salvadora persica; extraction; minimum inhibitory concentration; minimal bactericidal concentration

Share and Cite

MDPI and ACS Style

Mirkamandar, E.; Shakibaie, M.R.; Adeli, S.; Mehrabani, M.; Hayatbakhsh, M.M.; Esmailian, S. In Vitro Antimicrobial Activity of Salvadora persica Extract on Helicobacter pylori Strains Isolated from Duodenal Ulcer Biopsies. Microbiol. Res. 2012, 3, e9. https://doi.org/10.4081/mr.2012.e9

AMA Style

Mirkamandar E, Shakibaie MR, Adeli S, Mehrabani M, Hayatbakhsh MM, Esmailian S. In Vitro Antimicrobial Activity of Salvadora persica Extract on Helicobacter pylori Strains Isolated from Duodenal Ulcer Biopsies. Microbiology Research. 2012; 3(1):e9. https://doi.org/10.4081/mr.2012.e9

Chicago/Turabian Style

Mirkamandar, Ehsan, Mohammad Reza Shakibaie, Saeed Adeli, Mitra Mehrabani, Mohammad Mehdi Hayatbakhsh, and Saeed Esmailian. 2012. "In Vitro Antimicrobial Activity of Salvadora persica Extract on Helicobacter pylori Strains Isolated from Duodenal Ulcer Biopsies" Microbiology Research 3, no. 1: e9. https://doi.org/10.4081/mr.2012.e9

APA Style

Mirkamandar, E., Shakibaie, M. R., Adeli, S., Mehrabani, M., Hayatbakhsh, M. M., & Esmailian, S. (2012). In Vitro Antimicrobial Activity of Salvadora persica Extract on Helicobacter pylori Strains Isolated from Duodenal Ulcer Biopsies. Microbiology Research, 3(1), e9. https://doi.org/10.4081/mr.2012.e9

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