Irisin Role in Chondrocyte 3D Culture Differentiation and Its Possible Applications
Abstract
:1. Introduction
2. Materials and Methods
2.1. Irisin Treatment
2.2. Chondrocyte Pellet Cultures
2.3. Real-Time PCR
- sense Sox-9 5′-GCTCTGGAGACTTCTGAACGAGAG-3′;
- antisense Sox-9 5′-CGTTCTTCACCGACTTCCTCC-3′;
- sense Aggrecan 5′-GACTTCCGCTGGTCAGATGG-3′;
- antisense Aggrecan 5′-RCGTTTGTAGGTGGTGGCTGTG-3′;
- sense Collagen I 5′-TGAAGGGACACAGAGGTTTCAG-3′;
- antisense Collagen I 5′-GTAGCACCATCATTTCCACGA-3′;
- sense Collagen II 5′-CATGAGGGCGCGGTAGAGAC-3′;
- antisense Collagen II 5′-TGCCAGCCTCCTGGACATC-3′;
- sense Collagen X 5′-AACTCCCAGCACGCAGAATCC-3′;
- antisense Collagen X 5′- GGCATTTGGTATCGTTCAGCG-3′;
- sense β-actin 5′-AATCGTGCGTGACATTAAG-3′;
- antisense β-actin 5′-GAAGGAAGGCTGGAAGAG-3′;
- sense β2 microglobulin (B2M) 5′-ATGAGTATGCCTGCCGTGTGA-3′;
- antisense β2 microglobulin 5′-GGCATCTTCAAACCTCCATG-3′;
2.4. Western Blot
2.5. Histological Analysis
2.6. Immunofluorescence of Pellet Sections
2.7. Statistical Analysis
3. Results
3.1. Extracellular Signal-Regulated Kinase (ERK) Phosphorylation in HACs Treated with Irisin
3.2. Irisin Treatment Increases Sox-9, Aggrecan, and Collagen II Expression in Chondrocyte Pellets
3.3. Effects of Irisin on the mRNA Expression of Chondrogenic Markers
3.4. Irisin Stimulates Chondrocyte Differentiation and Tissue Growth
3.5. Irisin Modulates HACs Chondrogenic Differentiation
3.6. Irisin Does Not Affect the Expression of Aggrecan in HAC Pellets
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Posa, F.; Zerlotin, R.; Ariano, A.; Cosola, M.D.; Colaianni, G.; Fazio, A.D.; Colucci, S.; Grano, M.; Mori, G. Irisin Role in Chondrocyte 3D Culture Differentiation and Its Possible Applications. Pharmaceutics 2023, 15, 585. https://doi.org/10.3390/pharmaceutics15020585
Posa F, Zerlotin R, Ariano A, Cosola MD, Colaianni G, Fazio AD, Colucci S, Grano M, Mori G. Irisin Role in Chondrocyte 3D Culture Differentiation and Its Possible Applications. Pharmaceutics. 2023; 15(2):585. https://doi.org/10.3390/pharmaceutics15020585
Chicago/Turabian StylePosa, Francesca, Roberta Zerlotin, Anastasia Ariano, Michele Di Cosola, Graziana Colaianni, Aldo Di Fazio, Silvia Colucci, Maria Grano, and Giorgio Mori. 2023. "Irisin Role in Chondrocyte 3D Culture Differentiation and Its Possible Applications" Pharmaceutics 15, no. 2: 585. https://doi.org/10.3390/pharmaceutics15020585