Seed Transmission of Beet Curly Top Virus and Beet Curly Top Iran Virus in a Local Cultivar of Petunia in Iran
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sources of Petunia Seeds and Virus Isolates
2.2. Seed Transmission Experiments
2.3. DNA Extraction, PCR Analysis and Sequencing
2.4. Immunocapture PCR
2.5. DIG Southern Blot Hybridization Analysis
2.6. Seed Transmission Rate of BCTV-Svr and BCTIV
3. Results
3.1. Detection of BCTV-Svr and BCTIV in Petunia Seeds and Their Developed Symptomatic Plants
3.2. Vertical Transmission of BCTV-Svr and BCTIV through Three Generations of Petunia
3.3. Presence of Virion Particles in the Seedlings and Seeds
3.4. BCTV-Svr Infection of Seeds in Agroinfected Petunia Plants
3.5. Southern Blot Analysis of BCTV-Svr-Infected Plants
3.6. Seed Transmission Rate of BCTV-Svr and BCTIV
4. Discussion
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Primer | * Nucleotide Position | Sequence from 5′ to 3′ | Expected Product Size (bp) |
---|---|---|---|
BCTV-Svr358V | 358–383 | GTGGATCAATTTCCAGACAATTATC | 520 |
BCTV-Svr877C | 853–877 | CCCCATAAGAGCCATATCAAACTTC | |
BCTIV474V | 474–493 | TACAAGAAGTATGGCGGTTC | 682 |
BCTIV1155C | 1134–1155 | AAGAATAGCATTCTCCTTCAC |
Experiment | Sampling Stage | Petunia Cultivar | Infected/Tested (Percentage of Infection) | ||
---|---|---|---|---|---|
BCTV-Svr | BCTIV | Mixed Infection | |||
1 | 4–6 leaf | local | 13/34 (38.2%) | 3/34 (8.8%) | 1/34 (2.9%) |
PCSP | 0/23 (0%) | 0/23 (0%) | 0/23 (0%) | ||
2 | flowering | local | 39/50 (78.0%) | 5/50 (10.0%) | 2/50 (4.0%) |
PCSP | 0/56 (0%) | 0/56 (0%) | 0/56 (0%) | ||
3 | flowering | local | 49/70 (70.0%) | 13/70 (18.5%) | 3/70 (4.2%) |
PCSP | 0/70 (0%) | 0/70 (0%) | 0/70 (0%) |
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Anabestani, A.; Behjatnia, S.A.A.; Izadpanah, K.; Tabein, S.; Accotto, G.P. Seed Transmission of Beet Curly Top Virus and Beet Curly Top Iran Virus in a Local Cultivar of Petunia in Iran. Viruses 2017, 9, 299. https://doi.org/10.3390/v9100299
Anabestani A, Behjatnia SAA, Izadpanah K, Tabein S, Accotto GP. Seed Transmission of Beet Curly Top Virus and Beet Curly Top Iran Virus in a Local Cultivar of Petunia in Iran. Viruses. 2017; 9(10):299. https://doi.org/10.3390/v9100299
Chicago/Turabian StyleAnabestani, Ameneh, Seyed Ali Akbar Behjatnia, Keramat Izadpanah, Saeid Tabein, and Gian Paolo Accotto. 2017. "Seed Transmission of Beet Curly Top Virus and Beet Curly Top Iran Virus in a Local Cultivar of Petunia in Iran" Viruses 9, no. 10: 299. https://doi.org/10.3390/v9100299