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Viruses 2016, 8(7), 181;

Development of Neutralization Assay Using an eGFP Chikungunya Virus

Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
Ningbo International Travel Healthcare Center, Ningbo 315012, China
School of Medicine, Hunan Normal University, Changsha 410000, China
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Luis Martinez-Sobrido
Received: 2 March 2016 / Revised: 31 May 2016 / Accepted: 23 June 2016 / Published: 28 June 2016
(This article belongs to the Special Issue Replication-Competent Reporter-Expressing Viruses)
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Chikungunya virus (CHIKV), a member of the Alphavirus genus, is an important human emerging/re-emerging pathogen. Currently, there are no effective antiviral drugs or vaccines against CHIKV infection. Herein, we construct an infectious clone of CHIKV and an eGFP reporter CHIKV (eGFP-CHIKV) with an isolated strain (assigned to Asian lineage) from CHIKV-infected patients. The eGFP-CHIKV reporter virus allows for direct visualization of viral replication through the levels of eGFP expression. Using a known CHIKV inhibitor, ribavirin, we confirmed that the eGFP-CHIKV reporter virus could be used to identify inhibitors against CHIKV. Importantly, we developed a novel and reliable eGFP-CHIKV reporter virus-based neutralization assay that could be used for rapid screening neutralizing antibodies against CHIKV. View Full-Text
Keywords: Chikungunya virus; reporter virus; antiviral; neutralization antibody Chikungunya virus; reporter virus; antiviral; neutralization antibody

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Deng, C.-L.; Liu, S.-Q.; Zhou, D.-G.; Xu, L.-L.; Li, X.-D.; Zhang, P.-T.; Li, P.-H.; Ye, H.-Q.; Wei, H.-P.; Yuan, Z.-M.; Qin, C.-F.; Zhang, B. Development of Neutralization Assay Using an eGFP Chikungunya Virus. Viruses 2016, 8, 181.

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