Viruses 2016, 8(7), 181; https://doi.org/10.3390/v8070181
Development of Neutralization Assay Using an eGFP Chikungunya Virus
1
Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
2
Ningbo International Travel Healthcare Center, Ningbo 315012, China
3
School of Medicine, Hunan Normal University, Changsha 410000, China
4
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China
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These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Luis Martinez-Sobrido
Received: 2 March 2016 / Revised: 31 May 2016 / Accepted: 23 June 2016 / Published: 28 June 2016
(This article belongs to the Special Issue Replication-Competent Reporter-Expressing Viruses)
Abstract
Chikungunya virus (CHIKV), a member of the Alphavirus genus, is an important human emerging/re-emerging pathogen. Currently, there are no effective antiviral drugs or vaccines against CHIKV infection. Herein, we construct an infectious clone of CHIKV and an eGFP reporter CHIKV (eGFP-CHIKV) with an isolated strain (assigned to Asian lineage) from CHIKV-infected patients. The eGFP-CHIKV reporter virus allows for direct visualization of viral replication through the levels of eGFP expression. Using a known CHIKV inhibitor, ribavirin, we confirmed that the eGFP-CHIKV reporter virus could be used to identify inhibitors against CHIKV. Importantly, we developed a novel and reliable eGFP-CHIKV reporter virus-based neutralization assay that could be used for rapid screening neutralizing antibodies against CHIKV. View Full-TextKeywords:
Chikungunya virus; reporter virus; antiviral; neutralization antibody
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MDPI and ACS Style
Deng, C.-L.; Liu, S.-Q.; Zhou, D.-G.; Xu, L.-L.; Li, X.-D.; Zhang, P.-T.; Li, P.-H.; Ye, H.-Q.; Wei, H.-P.; Yuan, Z.-M.; Qin, C.-F.; Zhang, B. Development of Neutralization Assay Using an eGFP Chikungunya Virus. Viruses 2016, 8, 181.
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