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Replication-Competent Influenza A Viruses Expressing Reporter Genes
Article

Development of Neutralization Assay Using an eGFP Chikungunya Virus

1
Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
2
Ningbo International Travel Healthcare Center, Ningbo 315012, China
3
School of Medicine, Hunan Normal University, Changsha 410000, China
4
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Luis Martinez-Sobrido
Viruses 2016, 8(7), 181; https://doi.org/10.3390/v8070181
Received: 2 March 2016 / Revised: 31 May 2016 / Accepted: 23 June 2016 / Published: 28 June 2016
(This article belongs to the Special Issue Replication-Competent Reporter-Expressing Viruses)
Chikungunya virus (CHIKV), a member of the Alphavirus genus, is an important human emerging/re-emerging pathogen. Currently, there are no effective antiviral drugs or vaccines against CHIKV infection. Herein, we construct an infectious clone of CHIKV and an eGFP reporter CHIKV (eGFP-CHIKV) with an isolated strain (assigned to Asian lineage) from CHIKV-infected patients. The eGFP-CHIKV reporter virus allows for direct visualization of viral replication through the levels of eGFP expression. Using a known CHIKV inhibitor, ribavirin, we confirmed that the eGFP-CHIKV reporter virus could be used to identify inhibitors against CHIKV. Importantly, we developed a novel and reliable eGFP-CHIKV reporter virus-based neutralization assay that could be used for rapid screening neutralizing antibodies against CHIKV. View Full-Text
Keywords: Chikungunya virus; reporter virus; antiviral; neutralization antibody Chikungunya virus; reporter virus; antiviral; neutralization antibody
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MDPI and ACS Style

Deng, C.-L.; Liu, S.-Q.; Zhou, D.-G.; Xu, L.-L.; Li, X.-D.; Zhang, P.-T.; Li, P.-H.; Ye, H.-Q.; Wei, H.-P.; Yuan, Z.-M.; Qin, C.-F.; Zhang, B. Development of Neutralization Assay Using an eGFP Chikungunya Virus. Viruses 2016, 8, 181. https://doi.org/10.3390/v8070181

AMA Style

Deng C-L, Liu S-Q, Zhou D-G, Xu L-L, Li X-D, Zhang P-T, Li P-H, Ye H-Q, Wei H-P, Yuan Z-M, Qin C-F, Zhang B. Development of Neutralization Assay Using an eGFP Chikungunya Virus. Viruses. 2016; 8(7):181. https://doi.org/10.3390/v8070181

Chicago/Turabian Style

Deng, Cheng-Lin, Si-Qing Liu, Dong-Gen Zhou, Lin-Lin Xu, Xiao-Dan Li, Pan-Tao Zhang, Peng-Hui Li, Han-Qing Ye, Hong-Ping Wei, Zhi-Ming Yuan, Cheng-Feng Qin, and Bo Zhang. 2016. "Development of Neutralization Assay Using an eGFP Chikungunya Virus" Viruses 8, no. 7: 181. https://doi.org/10.3390/v8070181

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