Isolation and Genetic Characterization of Genotype VII Velogenic Pathotype Newcastle Disease Virus from Commercial Chicken Farms in Central Ethiopia, Distinct from the Local Vaccine Strains
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Area
2.2. Study Population
2.3. Study Design
2.4. Sample Size Determination and Sampling
2.5. Data Collection
Questionnaire Survey
2.6. Clinical and Postmortem Examination
2.7. Sample Collection, Transportation, and Storage
2.8. Isolation and Molecular Detection of ND Virus
2.9. Sequencing and Phylogenetic Analysis
2.10. Pathogenicity Assessment
2.11. Data Management and Statistical Analysis
3. Results
3.1. Questionnaire Survey
3.2. Clinical and Postmortem Findings
3.3. Isolation of ND Virus
3.4. Haemagglutination Test
3.5. Molecular Detection of ND Field Viruses
3.6. Sequence Analysis of ND Field Virus Isolates
3.7. Phylogenetic Analysis of ND Viruses
3.8. Pathogenicity of ND Virus Filed Isolates
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Farm # | Age (Days) | Breed | Production Type | Flock Size | Location |
---|---|---|---|---|---|
1 | 31 | Rose 308 | Broilers | 10,000 | Bishoftu |
2 | 35 | Lohman Brown | Layers | 10,000 | Bishoftu |
3 | 25 | Cobb 500 | Broilers | 2000 | Mojo |
4 | 29 | Bovans brown | Layers | 4000 | Mojo |
5 | 28 | Sasso | Dual purpose | 3000 | Addis Ababa |
6 | 62 | Sasso | Dual purpose | 500 | Bishoftu |
Farm # | Location | No. of Susceptible Chicken | Morbidity (%) | Mortality (%) | Case Fatality Rate (%) |
---|---|---|---|---|---|
1 | Bishoftu | 10,000 | 1300 (13%) | 700 (7%) | 53.8% |
2 | Bishoftu | 10,000 | 1450 (14.5%) | 1000 (10%) | 68.9% |
3 | Mojo | 2000 | 1000 (50%) | 542 (27.1%) | 54.2% |
4 | Mojo | 4000 | 2000 (50%) | 1700 (42.5%) | 85.0% |
5 | Addis Ababa | 3000 | 950 (31%) | 800 (26.6%) | 84.2% |
6 | Bishoftu | 500 | None | None | None |
Total | 29,000 | 6700 (23.1%) | 4742 (16.3%) | 70.8% |
Farm # | Breed | Production Type | Clinical Signs Observed |
---|---|---|---|
1 | Rose 308 | Broilers | Respiratory distress with gasping, depression, paralysis of the legs, and greenish diarrhoea |
2 | Lohman Brown | Layers | Torticollis, circling, and depression |
3 | Cobb 500 | Broilers | Respiratory distress with gasping, depression, greenish diarrhoea, and torticollis |
4 | Bovans brown | Layers | Ocular discharge, greenish diarrhoea, and depression |
5 | Sasso | Dual purpose | Depression, greenish diarrhoea, and ocular discharge |
6 | Sasso | Dual purpose | Depression, torticollis, and greenish diarrhoea |
Farm Code | Sample Code | No of Eggs Died | Mortality (%) | Time of Embryo Death (hr) | HA Test Result | HA Titre | PCR Result | Mean Death Time (hr) |
---|---|---|---|---|---|---|---|---|
Farm 1 | 1 | 3, P1 | 75 | 48 (2), 72 (1) | Pos | 128 | Pos | 42 |
2 | 3, P1 | 75 | 48 (2), 72 (1) | Pos | 128 | Pos | 51.4 | |
3 | 4, P1 | 100 | 48 (2), 72 (2) | Pos | 512 | Pos | 45 | |
4 | 0 | 0 | - | Neg | Neg | - | ||
5 | 1, P3 | 25 | 96 | Neg | Neg | - | ||
Farm 2 | 6 | 4, P1 | 100 | 48 (3), 72 (1) | Pos | 1024 | Pos | 41 |
7 | 2, P1 | 50 | 96 (1), 96 (1) | Neg | Neg | - | ||
8 | 0 | 0 | - | Neg | Neg | - | ||
9 | 1, P3 | 25 | 72 (1) | Neg | Neg | - | ||
10 | 0 | 0 | - | Neg | Neg | - | ||
Farm 3 | 11 | 0 | 0 | - | Neg | Neg | - | |
12 | 4, P1 | 100 | 48 (2), 72 (2) | Pos | 512 | Pos | 57 | |
13 | 3, P1 | 75 | 48 (2), 72 (1) | Pos | 128 | Pos | 49 | |
14 | 1, P3 | 25 | 96 (1) | Neg | Neg | - | ||
15 | 0 | 0 | - | Neg | Neg | - | ||
Farm 4 | 16 | 1, P2 | 50 | 48 (2) | Neg | Neg | - | |
17 | 0 | 0 | - | Neg | Neg | - | ||
18 | 0 | 0 | - | Neg | Neg | - | ||
19 | 1, P3 | 25 | 96 (1) | Neg | Neg | - | ||
20 | 4, P1 | 100 | 48 (3), 72 (1) | Pos | 1024 | Pos | 43 | |
Farm 5 | 26 | 3, P1 | 75 | 48 (2), 72 (1) | Pos | 512 | Pos | 56 |
27 | 2, P2 | 50 | 48 (1), 72 (1) | Pos | 16 | Pos | 48 | |
28 | 3, P1 | 75 | 48 (1), 72 (2) | Pos | 64 | Pos | 52 | |
29 | 2, P2 | 50 | 48 (1), 96 (1) | Pos | 16 | Pos | 42 | |
30 | 1, P3 | 25 | 96 (1) | Neg | Neg | - | ||
Farm 6 | 21 | 0 | 0 | - | Neg | Neg | - | |
22 | 0 | 0 | - | Neg | Neg | - | ||
23 | 1, P1 | 25 | 96 (1) | Neg | Neg | - | ||
24 | 0 | 0 | - | Neg | Neg | - | ||
25 | 0 | 0 | - | Neg | Neg | - | ||
Neg control | 31 | 0 | 0 | - | Neg | - | - | No death |
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Yadeta, W.; Amosun, E.; Mohammed, H.; Woldemedhin, W.; Sherefa, K.; Legesse, A.; Deresse, G.; Birhanu, K.; Abayneh, T.; Getachew, B.; et al. Isolation and Genetic Characterization of Genotype VII Velogenic Pathotype Newcastle Disease Virus from Commercial Chicken Farms in Central Ethiopia, Distinct from the Local Vaccine Strains. Viruses 2024, 16, 1249. https://doi.org/10.3390/v16081249
Yadeta W, Amosun E, Mohammed H, Woldemedhin W, Sherefa K, Legesse A, Deresse G, Birhanu K, Abayneh T, Getachew B, et al. Isolation and Genetic Characterization of Genotype VII Velogenic Pathotype Newcastle Disease Virus from Commercial Chicken Farms in Central Ethiopia, Distinct from the Local Vaccine Strains. Viruses. 2024; 16(8):1249. https://doi.org/10.3390/v16081249
Chicago/Turabian StyleYadeta, Waktole, Elizabeth Amosun, Hawa Mohammed, Wubet Woldemedhin, Kedir Sherefa, Abinet Legesse, Getaw Deresse, Kenaw Birhanu, Takele Abayneh, Belayneh Getachew, and et al. 2024. "Isolation and Genetic Characterization of Genotype VII Velogenic Pathotype Newcastle Disease Virus from Commercial Chicken Farms in Central Ethiopia, Distinct from the Local Vaccine Strains" Viruses 16, no. 8: 1249. https://doi.org/10.3390/v16081249
APA StyleYadeta, W., Amosun, E., Mohammed, H., Woldemedhin, W., Sherefa, K., Legesse, A., Deresse, G., Birhanu, K., Abayneh, T., Getachew, B., Farnós, O., Kamen, A. A., & Gelaye, E. (2024). Isolation and Genetic Characterization of Genotype VII Velogenic Pathotype Newcastle Disease Virus from Commercial Chicken Farms in Central Ethiopia, Distinct from the Local Vaccine Strains. Viruses, 16(8), 1249. https://doi.org/10.3390/v16081249