Next Article in Journal
Expression of a Functional Mx1 Protein Is Essential for the Ability of RIG-I Agonist Prophylaxis to Provide Potent and Long-Lasting Protection in a Mouse Model of Influenza A Virus Infection
Next Article in Special Issue
Juvenile Wels Catfish (Silurus glanis) Display Age-Related Mortality to European Catfish Virus (ECV) under Experimental Conditions
Previous Article in Journal
Clinical, Virological and Immunological Responses after Experimental Infection with African Horse Sickness Virus Serotype 9 in Immunologically Naïve and Vaccinated Horses
Previous Article in Special Issue
Seroepidemiological Survey of West Nile Virus Infections in Horses from Berlin/Brandenburg and North Rhine-Westphalia, Germany
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Viruses
Volume 14
Issue 7
10.3390/v14071546
viruses-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Review

Fish Innate Immune Response to Viral Infection—An Overview of Five Major Antiviral Genes

by
Maria del Mar Ortega-Villaizan
,
Veronica Chico
and
Luis Perez
*
Instituto de Investigación Desarrollo e Innovación en Biotecnología Sanitaria de Elche (IDiBE—UMH), 03202 Elche, Spain
*
Author to whom correspondence should be addressed.
Viruses 2022, 14(7), 1546; https://doi.org/10.3390/v14071546
Submission received: 31 May 2022 / Revised: 29 June 2022 / Accepted: 11 July 2022 / Published: 15 July 2022
(This article belongs to the Special Issue Fish Virology)
Versions Notes

Abstract

:
Fish viral diseases represent a constant threat to aquaculture production. Thus, a better understanding of the cellular mechanisms involved in establishing an antiviral state associated with protection against virus replication and pathogenesis is paramount for a sustainable aquaculture industry. This review summarizes the current state of knowledge on five selected host innate immune-related genes in response to the most relevant viral pathogens in fish farming. Viruses have been classified as ssRNA, dsRNA, and dsDNA according to their genomes, in order to shed light on what those viruses may share in common and what response may be virus-specific, both in vitro (cell culture) as well as in vivo. Special emphasis has been put on trying to identify markers of resistance to viral pathogenesis. That is, those genes more often associated with protection against viral disease, a key issue bearing in mind potential applications into the aquaculture industry.

1. Introduction

Following virus challenge, fish mount an early antiviral response by activating the innate immune system [1,2,3] and later on another response takes place in the way of antibody production [4,5] and cytotoxic T-cell immunity [6]. Here, the focus will be set on the activation of five genes in the interferon pathway that could be regarded as the most relevant in the response to viral infection in fish. The five selected genes are the most widely reported virus-induced genes associated with antiviral activity in fish: type I interferon (ifn) [7] the myxovirus resistant protein (mx) [8], the interferon stimulated gene 15 (isg15) [9], viperin (also named vig, rsda2) [10], and the grass carp reovirus induced gene (gig) [11].
Interferons (IFN) are secreted proteins in the range of 20–23 KDa. After binding to a receptor on the cell membrane, they activate a number of cellular pathways that ultimately lead to the transcriptional activation of the interferon-stimulated genes (ISGs) [7]. Two types of interferons have been found in teleost fish: type I and type II [12,13]. In this work, the term interferon (ifn) will refer to type I interferons (IFNα and IFNβ).
Mx proteins (60–70 KDa) belong to the superfamily of interferon-induced GTPases. Mx genes are highly polymorphic, with three Mx isoforms (Mx1, Mx2, Mx3) in salmonids and perciforms, and up to seven isoforms (MxA to MxG) in zebrafish [8]. ISG15 is a highly conserved ubiquitin-like protein (15 KDa) with antiviral activity [9,14]. Originally reported as a VHSV-induced gene (vig) in rainbow trout leukocytes [15,16], Viperin (42 KDa) is one ISG with direct antiviral activity [10]. Unlike the other four antiviral genes that are present in all vertebrates, Gig proteins are specific for fish. They display good antiviral activity and may be induced both in an ifn-dependent as well as an ifn-independent way [11].
There is an extensive body of literature on the activation of the innate immune response following viral infection of fish. A fair number of reviews have covered this subject, focusing either on one specific virus or one fish species [17,18,19]. A large portion of the data available on fish immune response against viral infection has been drawn from studies conducted on cyprinid and salmonid fish species. This is not surprising, since carp and salmon are arguably amongst the most widely cultivated fish species globally [20]. Thus, specific sections dealing with those two groups of fish will be presented, while other fish will be discussed together in a separate section. “Other fish” will mainly refer to orders Perciformes (i.e., sea bass, sea bream, tilapia) and Pleuronectiformes (flat fish species).

2. ssRNA Viruses

Viruses with single-stranded RNA (ssRNA) genomes comprise the largest number of viruses reported to cause disease in farmed fish, including long time known pathogens of carp (spring viremia of carp virus, SVCV) and pathogens affecting salmon such as salmon infectious hematopoietic necrosis virus (IHNV), infectious salmon anemia virus (ISAV), salmonid alphavirus (SAV), and viral hemorrhagic septicemia virus (VHSV) (Table 1).

2.1. ssRNA Virus Infection of Cyprinid Fish

Rhabdoviruses are arguably one of the viral families with greater impact on farmed fish. With respect to cyprinid species, SVCV is the most widely spread viral pathogen [21]. SVCV has been shown to be a powerful inducer of ifn expression in carp cells [22,23]. Likewise, the up-regulation of ifn gene expression by SVCV infection has been found in zebrafish cells [24,25,26]. Also in SVCV-infected zebrafish cells, mx gene transcription appears to be activated as early as 1hpi [27], suggesting an interferon-independent stimulation of mx in this case. In some instances, even an abortive infection with a fish rhabdovirus can lead to higher ifn and mx expression in cultured cells [28]. The stimulation of the vig/viperin gene expression has been reported in SVCV-infected cells [29,30,31,32]. SVCV infection has been also shown to up-regulate the transcription of the gig gene and the synthesis of GIG protein in zebrafish cells [33].
Along with the in vitro studies on cell culture, the in vivo interferon response of fish to rhabdoviruses has been largely examined in a number of cyprinid fish species. Increased levels of ifn and mx gene expression have been reported in carp and goldfish infected with SVCV, not only in internal organs, but also in skin [34,35,36,37].
Although not an aquacultured species, zebrafish (also a cyprinid fish) has been an extensively employed experimental model to investigate innate immune response to rhabdoviruses [38,39,40]. Overall, there is ample evidence of SVCV being a powerful inducer of all five genes (ifn, mx, isg15, vig/viperin, and gig) in zebrafish and carp [24,26,32,41,42,43]. Interestingly, in SVCV-infected zebrafish, some isoforms of the mx gene had their expression increased while other isoforms seemed to be down-regulated [25,44]. In contrast, none of the four zebrafish ifn genes were induced in larvae challenged with IHNV [45], which is suggestive of significant differences in the innate immune response between the larvae and adult state in fish. In fact, infections with IHNV and SVCV do trigger ifn expression in adult zebrafish [46]. Moreover, the levels of mx expression remain high in zebrafish that survived a VHSV challenge [38]. The stimulation of mx gene expression appeared particularly high in the gills [27] and liver [40] of the infected fish. Ifn expression was high in the kidney, whereas vig/viperin gene expression was found up-regulated in every tissue [47].

2.2. ssRNA Virus Infection of Salmonid Fish

SsRNA viruses are good inducers of ifn response in salmon cells [48]. Regarding the timing of gene expression, interferon-stimulated genes would be expected to be up-regulated after interferon induction, but that does not seem to be always the case. For instance, in salmon cells infected with ISAV, a peak of isg15 at 24 hpi is followed by high ifn expression at 48 hpi [49]. In contrast, a delayed (5dpi) up-regulation of both ifn and mx genes in SAV-infected salmon cells have been reported [50].
Infection with a virulent strain of VHSV provokes the transcriptional activation of ifn and mx in rainbow trout [51]. The ex-vivo transfection of rainbow trout red blood cells (RBCs) with a G-VHSV plasmid DNA vaccine has been reported to trigger mx expression [52]. The up-regulation of mx gene expression (with up to 1700-fold increment in liver) has been reported in salmonid fish infected by IHNV [53,54,55,56], as well as in salmon displaying clinical signs but not experiencing mortality [57]. ISAV infection of salmon also leads to increasing ifn and mx levels, albeit at later times (not until 6–8 days post challenge) [58,59]. This may be one of the cases where the innate response would be more a consequence of the ongoing viral replication rather than an early antiviral response of the fish, something that has been also suggested in IHNV-infected salmon [55]. Similar to viral infections of carp, the up-regulation of gig or gig-like gene expression has been reported in ISAV-infected salmon [60]. The transcriptional activation of gig along with that of mx and vig/viperin has been observed in salmon between 2 and 4 weeks after challenge with SAV [61,62,63].

2.3. ssRNA Virus Infection of Other Fish

Many species of fish other than cyprinid and salmonid fish are known to be susceptible to ssRNA virus infections, also displaying a subsequent activation of the interferon pathway. In Pleuronectiformes (flatfish species), a potent induction of ifn and mx gene expression have been detected after challenge with rhabdoviruses HIRRV (hirame novirhabdovirus) and VHSV [64,65,66,67,68], with up to 16.000-fold increment of ifn levels at day 3 after VHSV infection. Likewise, high RNA expression levels of mx has been measured in the kidney and spleen of VHSV-infected perch [69].
Nodaviruses pose a major threat to cultured marine fish worldwide, particularly perciforms species. Nervous necrosis virus (NNV)-infected seabass displays strong ifn, mx, and isg15 responses in 6–12 h both in the kidney and brain [70,71]. The up-regulation of mx has been reported in barramundi brain cells persistently infected with nodavirus [72,73]. On this regard, the capsid protein of nodaviruses has been pointed out to be a strong inducer of innate immune genes transcription [74].
Both ifn and mx responses have been detected in tilapia lake virus (TiLV)-infected tilapia and zebrafish larvae [75,76].

3. dsRNA Viruses and Poly I:C

The Birnaviridae and Reoviridae families of viruses with double-stranded RNA (dsRNA) genomes include two very important fish pathogens such as grass carp reovirus (GCRV) and IPNV (Table 1). DsRNA has been recognized as one molecular pattern sensed as “strange” by the cell. Thus, dsRNA is regarded as a very effective inducer of the innate immune cell response. In particular, the synthetic dsRNA polyinosinic-polycytidylic acid (poly I:C) has been so widely used to study the cell response to dsRNA that it deserves an inclusion in this section along the response to actual dsRNA viruses.

3.1. dsRNA Virus Infection and polyI:C in Cyprinid Fish

Poly I:C consistently induces a rapid and strong interferon response in carp and zebrafish cells [22,23,25,26,29,77]. Mx RNA levels increased significantly following epithelioma papulosum cyprini (EPC) cells treatment either with poly I:C or with conditioned medium from cells treated with poly I:C [78]. As proof that it is a good virus mimic, poly I:C has been shown to be an effective inducer of gig and vig/viperin gene expression in vitro [10,33,79]. It is worth mentioning that the gig gene was discovered precisely in reovirus-infected cells [11].
The infection of zebrafish cells with GCRV led to an increase in mx gene expression levels over 100-fold [27]. Notably, fish reoviruses have the remarkable capacity of inducing interferon and interferon-mediated response even when the viral particle has been inactivated [7,11,80,81], which is suggestive that the input of dsRNA may be sufficient to trigger the cell response without the need of viral replication. High transcription levels of mx that correlated with the production of interferon-like activity in supernatants of birnavirus-infected cyprinid fish cells has also been reported [82,83].
In vivo, poly I:C has been most frequently delivered to fish by intraperitoneal (i.p.) injection. The transcriptional activation of ifn and mx genes in head kidney following i.p. injection of poly I:C has been determined in a zebrafish model [84] with a peak of expression after 48 h [85]. Intramuscular injection has also been proven effective in triggering a host response to poly I:C in cyprinid fish [86].
GCRV infection elicits a transcriptional activation of mx and isg15 in the spleen and kidney of carp, with the more virulent strains being the more potent inducers [87,88]. Following GCRV infection of goldfish, ifn RNA levels were particularly high in the skin [37]. This finding would be an indication that epithelial tissues may have been overlooked in the study of the immune response in fish.

3.2. dsRNA Virus Infection and polyI:C inSalmonid Fish

Many viruses of salmonid fish have been shown to be inhibited in some degree by the treatment (usually prior to infection) of the cells with poly I:C. Depending on the particular cell line, cells can be simply exposed to the synthetic dsRNA, whereas in some other cases a transfection reagent was required for poly I:C to enter the cells and exert the antiviral effect. The latter is the case of the rainbow trout gill (RTgill) cell line where ifn gene expression was significantly increased after poly I:C transfection [89]. In salmon, chinook salmon embryo cells (CHSE-214) cells transfection is also required for poly I:C to trigger ifn, mx and vig/viperin transcription [90,91]. In other salmonid cell lines, simple exposure to poly I:C is sufficient to induce mx gene expression [92]. Taken together, these studies support the notion that a robust ifn/mx response would be the main factor underlying the antiviral state induced by poly I:C.
Some authors have investigated the molecular weight-dependent activity of poly I:C, finding that high molecular weight (HMW) poly I:C is a stronger inducer of mx than the low molecular weight (LMW) poly I:C in rainbow trout gonad (RTG-2) cells [93]. This was somehow expected since the HMW poly I:C is closer to the size of the viral dsRNA genomes. Indeed, viral dsRNA isolated from reovirus-infected cells has been proven an effective inducer of interferon [94].
Further support for the poly I:C treatment accurately mimicking viral infection in vitro, the birnavirus IPNV has been proven to be good inducer of antiviral genes (ifn and mx) in cells where IPNV can establish a productive infection [56,95]. Likewise, transcriptional activation of mx has been observed in rainbow trout cells infected with chum salmon reovirus (CSV) [96]. Interestingly, in the abortive infection of rainbow trout RBCs with IPNV, still ifn and mx gene expression was found up-regulated [97].
Considering the strong antiviral effect of poly I:C in cell culture, it is not surprising to find in the literature a number of attempts to prove the antiviral activity of poly I:C in the whole fish, with reports of successful application of poly I:C in VHSV-challenged fish [84,98]. It is worth mentioning that previous poly I:C inoculation resulted in higher anti-VHSV antibody production in flounder [98].
In piscine reovirus (PRV)-infected salmon, higher levels of ifn, mx, isg15, and vig/viperin genes expression have been determined [57,99]. PRV is one virus capable of establishing productive infection in RBCs where it triggers a rapid (as early as 1dpi) ifn response followed by a peak of mx expression [100].
The birnavirus IPNV has been a classic model of dsRNA virus infection in salmonids. Several authors have demonstrated that the RNA levels of ifn, mx, and gig genes were increased in salmon fish challenged with IPNV [59,101,102,103].
The less studied piscine myocarditis virus (PMCV) also induces a response in infected salmon that is highlighted by the overexpression of the antiviral gene vig/viperin [104].

3.3. dsRNA Virus Infection and poly I:C in Other Fish

Other groups of teleost fish respond to poly I:C stimulation in a similar way to that of cyprinid and salmonid fish. Catfish cell lines exhibit a rapid (in only 6 h after stimulation) up-regulation of ifn, mx, and isg15 after exposure to poly I:C both in vitro and in vivo [105,106]. In vivo, i.p. injection of poly I:C also correlates with increased mx and isg15 levels in rock bream and large yellow croaker [107,108].
Regarding the response to a dsRNA virus challenge, bluegill (Lepomis machrochirus) cells infected with infectious pancreatic necrosis virus (IPNV) show increased levels of ifn and mx from early times of infection [95].
There is a limited number of studies on fish other than cyprinids and salmonids. In one rare example on IPNV infection in cod causing death, the induction of isg15 following IPNV challenge has been reported [9]. In birnavirus-infected flounder, mx was overexpressed at the transcriptional level [109].

4. DNA Viruses

Like ssRNA and dsRNA, a pathogen–derived dsDNA can be sensed as “strange” by cell pattern recognition receptors and mount a reaction to that exogenous molecule [110]. The two major groups of fish DNA viruses are the Iridoviridae and Herpesviridae families.
The iridovirus LCDV (lymphocystis disease virus) activates mx gene expression in Senegalese sole [111] as well as other cytokines. High Mx protein levels in RBIV-infected turbot have been also reported [112]. In contrast, neither ifn nor mx mRNA levels were increased in sea bream infected with LCDV [113]. The activation of mx transcription has also been observed in mandarin fish challenged with infectious spleen and kidney necrosis virus (ISKNV) [114].
In herpesvirus-infected carp, the interferon response shows a good correlation with viral loads [47,115]. In any case, the innate immune response of fish to herpesviruses seems to be variable. While ifn expression levels did not show significant differences in cyprinid herpesvirus (CyHV)-infected carp [116], stimulation of mx transcription did occur in catfish infected with channel catfish virus (CCV) [106].

5. Comparative Summary of ssRNA, dsRNA and DNA Viruses

Molecular patterns associated to pathogens are recognized by the cell pattern recognition receptors. One group of receptors with a decisive role in sensing foreign ssRNA, dsRNA, or DNA are the toll-like receptors (TLRs). In fish as well as in other vertebrates, while TLR9 recognizes pathogen DNA, TLR3 is the receptor for foreign dsRNA, and both TLR7 and TLR8 would sense viral ssRNA [117].
Overall, from the available information in the literature, it can be concluded that ssRNA viruses and dsRNA viruses elicit a rather similar host innate immune response (Table S1). Discrepancies come up when comparing RNA and DNA viruses. Whereas the majority of the RNA viruses reviewed in the previous sections replicate in the cytoplasm, the life cycle of many DNA viruses requires the cell nucleus. Although there are exceptions to this principle (i.e., orthomyxoviruses such as ISAV with nuclear transcription and replication), it can be taken as a rule of thumb. Thus, for a given virus, having either a nuclear or a cytoplasmic life cycle may result in differences regarding the modulation of the innate immune response within the infected cell. The transcriptional activation of vig/viperin gene was first found only in RNA virus-infected fish, but later on the up-regulation of vig/viperin in iridovirus-infected fish was also reported [118,119]. Recently, microarray analysis has revealed the stimulation of gig in salmon infected with a poxvirus, in addition to mx, isg15, and vig/viperin [120].
As a general rule, RNA viruses tend to activate the transcription of a wider number of antiviral genes, while the response to DNA viruses seems restricted to fewer genes (Table 2). Four of the key innate immune genes (ifn, mx, isg15, vig/viperin) appear to be up-regulated by almost every RNA virus studied. In studies conducted with both types of viruses, differences between RNA and DNA viruses have been reported. For instance, in carp cells, the herpesvirus CyHV seems to be a poor inducer of interferon compared to SVCV [22]. This lack of IFN stimulation upon CyHV challenge has been verified in vivo [121].
With respect to poly I:C compared to viral RNA, some differences have been found as well regarding the cell response. In salmon TO cells, while poly I:C stimulation triggers first ifn gene expression followed hours later by an increase of mx RNA levels, ISAV infection resulted in a rapid overexpression of mx and isg15 genes followed by a late induction of ifn gene expression [122]. In some instances, the differences are related to the specific isoform of the gene that is activated; in rainbow trout, while poly I:C stimulated mx3 expression, VHSV induced mx1 and mx2 [123].

6. Correlation between Up-Regulation of Innate Immunity Genes and Protection from Viral Infection and Disease

As previously discussed, there is ample support for the activation of innate immune genes after viral challenge in farmed fish species. Now the key question is: Does it really matter? Does the interferon-mediated response guarantee protection against disease, limiting morbidity and ensuring fish survival? Let us evaluate the evidence.
One point that should be underscored in the first place: a robust interferon response does not necessarily mean survival to virus infection. SVCV is a potent inducer of interferon and Mx in carp cells, but the outcome after exposure to SVCV is the death of the infected cells nevertheless [22,124]. This situation has been confirmed in vivo, with high ifn and mx induction but also high mortality in SVCV-infected zebrafish [124,125]. The transcriptional induction of the mx gene is considered a hallmark of the antiviral response in fish. Challenge with a virulent strain of VHSV leads to high interferon and mx induction but also high mortality in infected rainbow trout [51]. Thus, in some virus-host situations, a potent ifn response would be more the result of increasing viral replication rather than an early mechanism of antiviral defense [55]. Salmonid fish cell lines such as RTG-2 have shown good responsiveness to VHSV infection. Interestingly, the low pathogenic strains of VHSV exhibit the higher stimulation capacity of mx gene expression, leading to the hypothesis that the highly pathogenic VHSV strains would be somehow able to restrict mx expression at least in vitro [126].
In contrast to viral infections in the whole fish, the transfection of cultured fish cells with plasmids encoding interferon or other interferon-stimulated antiviral genes has been usually successful in halting viral replication [14,36,80]. In one case, the inhibition was achieved by the overexpression of the antiviral gene gig in carp cells infected with the reovirus GCRV [80]. Conditioned medium from cells producing interferon has been in some instances capable of blocking viral (SVCV and GCRV) replication in cell culture [37,77], evidencing the synthesis and release of antiviral factors to the medium by the transfected cells.
Poly I:C has consistently demonstrated its capacity to protect (either completely or partially) cells from the virus-induced cytopathic effect. Examples of the antiviral activity of poly I:C are found in rainbow trout cells infected with VHSV [89,94,127], salmon cells infected with IPNV [90], and sea bream cells infected with birnavirus [128]. In contrast, poly I:C was not so successful in blocking nodavirus replication in grouper cells [129]. The same antiviral effect can be induced by in vitro synthesized dsRNA [79] or with isolated viral dsRNA [94]. The novel CRISPR/Cas genome-editing tool is currently assisting in the elucidation of the detailed mechanism underlying poly I:C induced antiviral effect, by knocking out candidate genes along the interferon pathway one by one [130,131,132].
The protective effect of poly I:C has been corroborated in vivo in zebrafish intraperitoneally injected with poly I:C and challenged with VHSV [85]. However, it appeared to be more a delay in virus-induced pathogenesis and mortality than actual protection [84]. In olive flounder, a drastic decrease of VHSV-induced mortality can be achieved by poly I:C administered intraperitoneally [133]. In a similar way, RBIV iridovirus replication in rock bream was inhibited by i.p. injection of poly I:C [107].
Intramuscular injection of turbot with a plasmid encoding interferon reduced the mortality caused by VHSV from 100% to 47%, in conjunction with a 200-fold up-regulation of mx gene expression in muscle [67]. Additionally, the high transcriptional levels of mx found in zebrafish survivors of a VHSV challenge may be associated with their high resistance to a second VHSV infection [38], although in this case the role of the adaptive immune response should not be neglected. In contrast, the injection of a mx gene encoding plasmid into turbot did not appear to induce any degree of protection against RIBV [112]. Correlation between higher interferon production and resistance to IPNV has been reported in Atlantic salmon [102]. However, the higher levels of mx and other interferon stimulated genes in IPNV-infected salmon may just be a result of the higher viral replication in susceptible fish [103]. Likewise, in SVCV-infected carp, a stronger interferon response was directly correlated with higher viral loads [47]. In contrast, zebrafish rag −/− mutants defective in adaptive immune response have been used to associate higher innate immune response with less susceptibility to SVCV infection [134].
Evidence in favor of the protective efficacy of the innate response has been found by analyzing the susceptibility to viral disease of selected populations of fish, where genetically resistant individuals seemed to exhibit a stronger innate immune response [135,136,137]. In some other instances, however, up-regulation of ifn, mx, and vig/viperin was associated with acute infection [55]. In tilapia infected with TiLV, higher mx response did not correlate with reduced infection [75].
Overall, the real protective effect of a strong ifn/ifn-induced gene response in vivo remains inconclusive, although there is a body of evidence pointing to the fact that a natural endogenous innate immune response would not suffice to stop virus spread in the organism.

7. Final Remarks

In the last decade, a large body of knowledge on the innate immune response in teleost fish has been built up by researchers on viral diseases of fish. The question is how the fish farming industry can benefit from all this available information.
As a starting point of thinking, viruses exist because they “learned” how to overcome the natural host immune response, reaching a situation where the production of interferon and other antiviral proteins become ineffective against virus replication within the host. However, that does not necessarily mean that viruses would prevail against a human- engineered, enhanced immune response elicited by a specific treatment or vaccine. Therefore, when thinking on prophylactic measures, we should not only focus on improving the capacity of producing neutralizing antibodies, but rather on triggering a rapid and robust antiviral state in the fish by means of the innate immune system. The experimental results reviewed in here indicate that the latter may be feasible. However, first there are some issues that will have to be sorted out. For instance, little information is available on the duration of the antiviral effect established through the interferon and related pathways. This is a key factor, since a long-lasting exacerbated innate response could be deleterious to the fish. Therefore, a novel way of thinking is that protection against disease in the long term might come from the more durable epigenetic changes in the host chromatin [41,137], resulting in a faster and enhanced response upon a second pathogen invasion.
Better understanding of the key responders to viral infection will boost the advancement of fish vaccinology. In that regard, the knowledge on some lesser-known areas such as mucosal immunity should be expanded. The use of omics techniques will contribute to provide an unbiased and comprehensive picture and may lead to the discovery of new players in the virus-host interplay. With such a multiplicity of factors playing a part in viral pathogenesis (e.g., species susceptibility, genetic variability among fish individuals, age/size of fish, water temperature, and virulence of viral isolates), establishing clear cut patterns is not going to be an easy task. This could be further complicated by the emergence of novel aquatic viruses under changing climatic conditions and increasing sea water temperatures in the coming years.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/v14071546/s1, Table S1: In vivo stimulation of the antiviral genes reviewed in this work in the main species and groups of fish.

Author Contributions

Conceptualization, L.P.; resources, L.P., M.d.M.O.-V., V.C.; data curation, L.P., M.d.M.O.-V., V.C.; writing—original draft preparation, L.P. writing—review and editing, L.P., M.d.M.O.-V., V.C.; supervision, L.P.; project administration, L.P.; funding acquisition, L.P., M.d.M.O.-V. All authors have read and agreed to the published version of the manuscript.

Funding

The authors acknowledge the financial support of Spain’s Ministerio de Ciencia y Competitividad Project RTI2018-096957-B-C22.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Acknowledgments

Maria E. Salvador (IDiBE-UMH) is acknowledged for her assistance in the management of the bibliography.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Levraud, J.P.; Jouneau, L.; Briolat, V.; Laghi, V.; Boudinot, P. IFN-stimulated genes in zebrafish and humans define an ancient arsenal of antiviral immunity. J. Immunol. 2019, 203, 3361–3373. [Google Scholar] [CrossRef] [PubMed]
  2. Robertsen, B. Expression of interferon and interferon-induced genes in salmonids in response to virus infection, interferon-inducing compounds and vaccination. Fish Shellfish Immunol. 2008, 25, 351–357. [Google Scholar] [CrossRef] [PubMed]
  3. Collet, B. Innate immune responses of salmonid fish to viral infections. Dev. Comp. Immunol. 2014, 43, 169–173. [Google Scholar] [CrossRef] [PubMed]
  4. Diaz-Rosales, P.; Muñoz-Atienza, E.; Tafalla, C. Role of teleost B cells in viral immunity. Fish Shellfish Immunol. 2019, 86, 135–142. [Google Scholar] [CrossRef]
  5. Munang’andu, H.M.; Mutoloki, S.; Evensen, O. Acquired immunity and vaccination against infectious pancreatic necrosis virus of salmon. Dev. Comp. Immunol. 2014, 43, 184–196. [Google Scholar] [CrossRef]
  6. Yamaguchi, T.; Takizawa, F.; Furihata, M.; Soto-Lampe, V.; Dijkstra, J.M.; Fischer, U. Teleost cytotoxic T cells. Fish Shellfish Immunol. 2019, 95, 422–439. [Google Scholar] [CrossRef]
  7. Yu, F.-F.; Zhang, Y.-B.; Liu, T.-K.; Liu, Y.; Sun, F.; Jiang, J.; Gui, J.-F. Fish virus-induced interferon exerts antiviral function through Stat1 pathway. Mol. Immunol. 2010, 47, 2330–2341. [Google Scholar] [CrossRef]
  8. Leong, J.C.; Trobridge, G.; Kim, C.H.; Johnson, M.; Simon, B. Interferon-inducible Mx proteins in fish. Immunol. Rev. 1998, 166, 349–363. [Google Scholar] [CrossRef]
  9. Das, B.K.; Collet, B.; Snow, M.; Ellis, A.E. Expression kinetics of ISG15 and viral major capsid protein (VP2) in Atlantic cod (Gadus morhua L.) fry following infection with infectious pancreatic necrosis virus (IPNV). Fish Shellfish Immunol. 2007, 23, 825–830. [Google Scholar] [CrossRef]
  10. Wang, F.; Jiao, H.; Liu, W.; Chen, B.; Wang, Y.; Chen, B.; Lu, Y.; Su, J.; Zhang, Y.; Liu, X. The antiviral mechanism of viperin and its splice variant in spring viremia of carp virus infected fathead minnow cells. Fish Shellfish Immunol. 2019, 86, 805–813. [Google Scholar] [CrossRef]
  11. Zhang, Y.B.; Gui, J.F. Identification of two novel interferon-stimulated genes from cultured CAB cells induced by UV-inactivated grass carp hemorrhage virus. Dis. Aquat. Org. 2004, 60, 1–9. [Google Scholar] [CrossRef] [Green Version]
  12. Gan, Z.; Chen, S.N.; Huang, B.; Zou, J.; Nie, P. Fish type I and type II interferons: Composition, receptor usage, production and function. Rev. Aquacult. 2019, 12, 773–804. [Google Scholar] [CrossRef] [Green Version]
  13. Stosik, M.; Tokarz-Deptula, B.; Deptula, W. Type I interferons in ray-finned fish (Actinopterygii). Fish Shellfish Immunol. 2021, 110, 35–43. [Google Scholar] [CrossRef] [PubMed]
  14. Langevin, C.; Van der Aa, L.M.; Houel, A.; Torhy, C.; Briolat, V.; Lunazzi, A.; Harmache, A.; Bremont, M.; Levraud, J.; Boudinot, P. Zebrafish ISG15 exerts a strong antiviral activity against RNA and DNA viruses and regulates interferon response. J. Virol. 2013, 87, 10025–10036. [Google Scholar] [CrossRef] [Green Version]
  15. Boudinot, P.; Massin, P.; Blanco, M.; Riffault, S.; Benmansour, A. vig-1, a new fish gene induced by the rhabdovirus glycoprotein, has a virus-induced homologue in humans and shares conserved motifs with the MoaA family. J. Virol. 1999, 73, 1846–1852. [Google Scholar] [CrossRef] [Green Version]
  16. Boudinot, P.; Salhi, S.; Blanco, M.; Benmansour, A. Viral haemorrhagic septicaemia virus induces vig-2, a new interferon responsive gene in rainbow trout. Fish. Shellfish Immunol. 2001, 11, 387–397. [Google Scholar] [CrossRef]
  17. Dahle, M.K.; Jorgensen, J.B. Antiviral defense in salmonid—Mission made possible? Fish Shellfish Immunol. 2019, 87, 421–437. [Google Scholar] [CrossRef]
  18. Sullivan, C.; Soos, B.L.; Millard, P.J.; Kim, C.H.; King, B.L. Modeling virus-induced inflammation in zebrafish: A balance between infection control and excessive inflammation. Front Immunol. 2021, 12, 636623. [Google Scholar] [CrossRef]
  19. Varela, M.; Figueras, A.; Novoa, B. Modelling viral infections using zebrafish: Innate immune response and antiviral research. Antiviral. Res. 2017, 139, 59–68. [Google Scholar] [CrossRef]
  20. FAO. The State of World Fisheries and Aquaculture 2018—Sustainability in Action; FAO: Rome, Italy, 2020. [Google Scholar]
  21. Ashraf, U.; Lu, Y.; Lin, L.; Yuan, J.; Wang, M.; Liu, X. Spring viremia of carp virus: Recent advances. J. Gen. Virol. 2016, 97, 1037–1051. [Google Scholar] [CrossRef]
  22. Adamek, M.; Rakus, K.; Chyb, J.; Brodgen, G.; Huebner, A.; Irnazarow, I.; Steinhagen, D. Interferon type I responses to virus infections in carp cells: In vitro studies on Cyprinid herpesvirus 3 and Rhabdovirus carpio infections. Fish Shellfish Immunol. 2012, 33, 482–493. [Google Scholar] [CrossRef] [PubMed]
  23. Wang, Z.; Xu, J.; Feng, J.; Wu, K.; Chen, K.; Jia, Z.; Zhu, X.; Huang, W.; Zhao, X.; Liu, Q.; et al. Structural and functional analyses of type I IFNa shed light into its interaction with multiple receptors in fish. Front Immunol. 2022, 13, 862764. [Google Scholar] [CrossRef] [PubMed]
  24. Feng, H.; Zhang, Q.-M.; Zhang, Y.; Li, Z.; Zhang, J.; Xiong, Y.-W.; Wu, M.; Gui, J.-F. Zebrafish IRF1, IRF3 and IRF7 differentially regulate IFNf1 and IFNf3 expression through assembly of homo- or heteroprotein complexes. J. Immunol. 2016, 197, 1893–1904. [Google Scholar] [CrossRef] [PubMed]
  25. Liu, X.; Cai, X.; Zhang, D.; Xu, C.; Xiao, W. Zebrafish foxo3b negatively regulates antiviral response through suppressing the transactivity of irf3 and irf7. J. Immunol. 2016, 197, 4736–4749. [Google Scholar] [CrossRef] [Green Version]
  26. Liu, S.B.; Lu, L.-F.; Lu, X.-B.; Li, S.; Zhang, Y.-A. Zebrafish FGR3 is a negative regulator of RLR pathway to decrease IFN expression. Fish Shellfish Immunol. 2019, 92, 224–229. [Google Scholar] [CrossRef]
  27. Ai, K.; Li, Y.; Hu, W.; Gao, W.; Fang, L.; Tian, G.; Ruan, G.; Xu, Q. Expression pattern analysis of IRF4 and its related genes revealed the functional differentiation of IRF4 paralogues in teleost. Fish Shellfish Immunol. 2017, 60, 59–64. [Google Scholar] [CrossRef]
  28. Wang, W.; Asim, M.; Yi, L.; Hegazy, A.M.; Hu, X.; Zhou, Y.; Ai, T.; Lin, L. Abortive infection of snakehead fish vesiculovirus in ZF4 cells was associated with the RLRs pathway activation by viral replicative intermediates. Int. J. Mol. Sci. 2015, 16, 6235–6250. [Google Scholar] [CrossRef] [Green Version]
  29. Li, S.; Lu, L.-F.; Wang, Z.-X.; Lu, X.-B.; Chen, D.-D.; Nie, P.; Zhang, Y.-A. The P protein of spring viremia of carp virus negatively regulates the fish interferon response by inhibiting the kinase activity of TANK-binding kinase 1. J. Virol. 2016, 90, 10728–10737. [Google Scholar] [CrossRef] [Green Version]
  30. Gong, X.Y.; Zhang, Q.M.; Gui, J.F.; Zhang, Y.B. SVCV infection triggers fish IFN response through RLR signaling pathway. Fish Shellfish Immunol. 2019, 86, 1058–1063. [Google Scholar] [CrossRef]
  31. Rakus, K.; Adamek, M.; Mojzesz, M.; Podlasz, P.; Chmielewska-Krzesinska, M.; Naumowicz, K.; Kasica-Jarosz, N.; Klak, K.; Rakers, S.; Way, K.; et al. Evaluation of zebrafish (Danio rerio) as an animal model for the viral infections of fish. J. Fish Diseases 2019, 42, 923–934. [Google Scholar] [CrossRef]
  32. Wang, Y.; Zhang, H.; Lu, Y.; Wang, F.; Liu, L.; Liu, J.; Liu, X. Comparative transcriptome analysis of zebrafish (Danio rerio) brain and spleen infected with spring viremia of carp virus (SVCV). Fish Shellfish Immunol. 2017, 69, 35–45. [Google Scholar] [CrossRef] [PubMed]
  33. Li, S.; Zhang, Y.-B.; Gui, J.-F.; Zhang, Q.-Y. Identification of DreI as an antiviral factor regulated by RLR signaling pathway. PLoS ONE 2012, 7, e32427. [Google Scholar] [CrossRef] [PubMed]
  34. Huang, Z.; Chen, S.; Liu, J.; Xiao, J.; Yan, J.; Feng, H. IFNa of black carp is an antiviral cytokine modified with N-linked glycosylation. Fish Shellfish Immunol. 2015, 46, 477–485. [Google Scholar] [CrossRef] [PubMed]
  35. Wei, X.; Li, X.; Zheng, X.; Jia, P.; Wang, J.; Yang, X.; Yu, L.; Shi, X.; Tong, G.; Liu, H. Toll-like receptors and interferon associated immune factors responses to spring viraemia of carp virus infection in common carp (Cyprinus carpio). Fish Shellfish Immunol. 2016, 55, 568–576. [Google Scholar] [CrossRef] [PubMed]
  36. Xiao, J.; Yan, J.; Chen, H.; Li, J.; Tian, Y.; Tang, L.; Feng, H. Mx1 of black carp functions importantly in the antiviral innate immune response. Fish Shellfish Immunol. 2016, 58, 584–592. [Google Scholar] [CrossRef]
  37. Yan, J.; Peng, L.; Li, Y.; Fan, H.; Tian, Y.; Liu, S.; Feng, H. IFNa of triploid hybrid of gold fish and allotetraploid is an antiviral cytokine against SVCV and GCRV. Fish Shellfish Immunol. 2016, 54, 529–536. [Google Scholar] [CrossRef]
  38. Estepa, A.; Coll, J. Innate multigene family memories are implicated in viral-survivor zebrafish phenotype. PLoS ONE 2015, 10, e0135483. [Google Scholar] [CrossRef]
  39. Jorgensen, L.V.G. Zebrafish as a model for fish diseases in aquaculture. Pathogens 2020, 9, 609. [Google Scholar] [CrossRef]
  40. Phelan, P.E.; Pressley, M.E.; Witten, P.E.; Mellon, M.T.; Blake, S.; Kim, C.H. Characterization of snakehead rhabdovirus infection in zebrafish (Danio rerio). J. Virol. 2005, 79, 1842–1852. [Google Scholar] [CrossRef] [Green Version]
  41. Medina-Gali, R.; Bello-Perez, M.; Martinez-Lopez, A.; Falco, A.; Ortega-Villaizan, M.; Encinar, J.A.; Novoa, B.; Coll, J.; Perez, L. Chromatin immunoprecipitation and high throughput sequencing of SVCV-infected zebrafish reveals novel epigenetic histone methylation patterns involved in antiviral immune response. Fish Shellfish Immunol. 2018, 82, 514–521. [Google Scholar] [CrossRef]
  42. Mojzesz, M.; Katarzyna, K.; Paulina, W.; Adamek, M.; Podlasz, P.; Chmielewska-Krzesinska, M.; Matras, M.; Reichert, M.; Chadzinska, M.; Rakus, K. Viral infection-iduced changes in the expression profile of non-RLR DExD/H-box RNA helicases (DDX1, DDX3, DHX9, DDX21 and DHX36) in zebrafish and common carp. Fish Shellfish Immunol. 2020, 104, 62–73. [Google Scholar] [CrossRef] [PubMed]
  43. Song, D.W.; Liu, G.L.; Xue, M.Y.; Qiu, T.X.; Wang, H.; Shan, L.P.; Liu, L.; Chen, J. In vitro and in vivo evaluation of antiviral activity of a phenylpropanoid derivative against spring viraemia of carp virus. Virus Res. 2021, 291, 198221. [Google Scholar] [CrossRef] [PubMed]
  44. Encinas, P.; Garcia-Valtanen, P.; Chinchilla, B.; Gomez-Casado, E.; Estepa, A.; Coll, J. Identification of multipath genes differentially expressed in pathway-targeted microarrays in zebrafish infected and surviving spring viremia carp virus (SVCV) suggest preventive drug candidates. PLoS ONE 2013, 8, e73553. [Google Scholar] [CrossRef]
  45. Briolat, V.; Jouneau, L.; Carvalho, R.; Palha, N.; Langevin, C.; Herbomel, P.; Schwartz, O.; Spaink, H.P.; Levraud, J.P.; Boudinot, P. Contrasted innate responses to two viruses in zebrafish: Insights into the ancestral repertoire of vertebrates IFN-stimulated genes. J. Immunol. 2014, 192, 4328–4341. [Google Scholar] [CrossRef] [Green Version]
  46. Aggad, D.; Mazel, M.; Boudinot, P.; Mogensen, K.E.; Hamming, O.J.; Hartmann, R.; Kotenko, S.; Herbomel, P.; Luftfalla, G.; Levraud, J. The two groups of zebrafish virus-induced interferons signal via distinct receptors with specific and shared chains. J. Immunol. 2009, 183, 3924–3931. [Google Scholar] [CrossRef] [Green Version]
  47. Adamek, M.; Matras, M.; Dawson, A.; Piackova, V.; Gela, D.; Kocour, M.; Adamek, J.; Kaminski, R.; Rakus, K.; Bergmann, S.M.; et al. Type I interferon responses of common carp strains with different levels of resistance to koi herpesvirus disease during infection with CyHV-3 or SVCV. Fish Shellfish Immunol. 2019, 87, 809–819. [Google Scholar] [CrossRef] [Green Version]
  48. Nerbovik, I.K.G.; Solheim, M.A.; Eggestol, H.O.; Ronneseth, A.; Jakobsen, R.A.; Wergeland, H.I.; Haugland, G.T. Molecular cloning of MDA5, phylogenetic analysis of RIG-I-like receptors (RLRs) and differential gene expression of RLRs, interferons and proinflammatory cytokines after in vitro challenge with IPNV, ISAV and SAV in the salmonid cell line TO. J. Fish Diseases 2017, 40, 1529–1544. [Google Scholar] [CrossRef]
  49. Schiotz, B.L.; Roos, N.; Rishovd, A.-L.; Gjoen, T. Formation of autophagosomes and redistribution of LC3 upon in vitro infection with infectious salmon anemia virus. Virus Res. 2010, 151, 104–107. [Google Scholar] [CrossRef]
  50. Sobhkhez, M.; Joensen, L.; Tollersrud, L.G.; Strandskog, G.; Thim, H.L.; Jorgensen, J.B. A conserved inhibitory role of supressor cytokine signaling 1 (SOCS1) in salmon antiviral immunity. Dev. Comp. Immunol. 2017, 67, 66–76. [Google Scholar] [CrossRef]
  51. Campbell, S.; McBeath, A.; Secombes, C.J.; Snow, M.; Collet, B. Interferon response following infection with genetically similar isolates of viral haemorrhagic septicaemia virus (VHSV) exhibiting contrasting virulence in rainbow trout. Fish Shellfish Immunol. 2011, 30, 287–294. [Google Scholar] [CrossRef]
  52. Puente-Marin, S.; Nombela, I.; Chico, V.; Ciordia, S.; Mena, M.C.; Coll, J.; Mercado, L.; Ortega-Villaizan, M. Rainbow trout erythrocytes ex vivo transfection with a DNA vaccine encoding VHSV glycoprotein G induces an antiviral immune response. Front. Immunol. 2018, 9, 2477. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  53. Müller, A.; Sutherland, B.J.G.; Koop, B.F.; Johnson, S.C.; Garver, K.A. Infectious hematopoietic necrosis virus (IHNV) persistence in Sockeye Salmon: Influence on brain transcriptome and subsequent response to the viral mimic poly (I:C). BMC Genomics 2015, 16, 634. [Google Scholar] [CrossRef] [PubMed]
  54. Overturf, K.; LaPatra, S.E. Quantitative expression of immunological factors in rainbow trout, Oncorhyncus mykiss (Walbaum), after infection with either Flavobacterium psychrophilum, Aeromonas salmonicida, or infectious haematopoietic necrosis virus. J. Fish Diseases 2006, 29, 215–224. [Google Scholar] [CrossRef] [PubMed]
  55. Polinski, M.P.; Bradshaw, J.C.; Rise, M.L.; Johnson, S.C.; Garver, K.A. Sockeye salmon demonstrate robust yet distinct transcriptomic kidney responses to rhabdovirus (IHNV) exposure and infection. Fish Shellfish Immunol. 2019, 94, 525–538. [Google Scholar] [CrossRef]
  56. Rodriguez Saint-Jean, S.; Perez-Prieto, S.S. Effects of salmonid fish viruses on Mx gene expression and resistance to single or dual viral infections. Fish Shellfish Immunol. 2007, 23, 390–400. [Google Scholar] [CrossRef]
  57. Polinski, M.P.; Bradshaw, J.C.; Inkpen, S.M.; Richard, J.; Fristvold, C.; Poppe, T.T.; Rise, M.L.; Garver, K.A.; Johnson, S.C. De novo assembly of Sockeye salmon kidney transcriptomes reveal a limited early response to piscine reovirus with or without infectious hematopoietic necrosis virus superinfection. BMC Genomics 2016, 17, 848. [Google Scholar] [CrossRef] [Green Version]
  58. Collet, B.; Urquhart, K.; Monte, M.; Collins, C.; García Perez, S.; Secombes, C.J.; Hall, B.E. Individual monitoring of immune response in Atlantic salmon Salmo salar following experimental infection with infectious salmon anaemia virus (ISAV). PLoS ONE 2015, 10, e0137767. [Google Scholar] [CrossRef] [Green Version]
  59. McBeath, A.J.A.; Snow, M.; Secombes, C.J.; Ellis, A.E.; Collet, B. Expression kinetics of interferon and interferon-induced genes in Atlantic salmon (Salmo salar) following infection with infectious pancreatic necrosis virus and infectious salmon anaemia virus. Fish Shellfish Immunol. 2007, 22, 230–241. [Google Scholar] [CrossRef]
  60. Valenzuela-Miranda, D.; Boltaña, S.; Cabrejos, M.E.; Yáñez, J.M.; Gallardo-Escárate, C. High-throughput transcriptome analysis of ISAV-infected Atlantic salmon Salmo salar unravels divergent immune responses associated to head-kidney, liver and gills tissues. Fish Shellfish Immunol. 2015, 45, 367–377. [Google Scholar] [CrossRef]
  61. Bela-ong, D.B.; Greiner-Tollersrud, L.; van der Wal, Y.A.; Jensen, I.; Seternes, O.M.; Jorgensen, J.B. Infection and microbial molecular motifs modulate transcription of the interferon-inducible gene ifit5 in a teleost fish. Dev. Comp. Immunol. 2020, 111, 103746. [Google Scholar] [CrossRef]
  62. Johansen, L.H.; Thim, H.L.; Jorgensen, S.M.; Afanasyev, S.; Strandskog, G.; Taksdal, T.; Fremmerlid, K.; McLoughlin, M.F.; Jorgensen, J.B.; Krasnov, A. Comparison of transcriptomic responses to pancreas disease (PD) and heart and skeletal muscle inflammation (HSMI) in heart of Atlantic salmon (Salmo salar L.). Fish Shellfish Immunol. 2015, 46, 612–623. [Google Scholar] [CrossRef] [PubMed]
  63. Robinson, N.A.; Krasnov, A.; Burgerhout, E.; Johnsen, H.; Moghadam, H.K.; Hillestad, B.; Aslam, M.L.; Baranski, M.; Boison, S.A. Response of the salmon heart transcriptome to pancreas disease: Differences between high- and low-ranking families for resistance. Sci. Rep. 2020, 10, 868. [Google Scholar] [CrossRef]
  64. Diaz-Rosales, P.; Romero, A.; Balseiro, P.; Dios, S.; Novoa, B.; Figueras, A. Microarray-based identification of differentially expressed genes in families of turbot (Scophtalmus maximus) after infection with viral haemorrhagic septicaemia virus (VHSV). Mar. Biotechnol. 2012, 14, 515–519. [Google Scholar] [CrossRef] [Green Version]
  65. Hwang, J.Y.; Lee, U.H.; Heo, M.J.; Jeong, J.M.; Kwon, M.G.; Jee, B.Y.; Park, C.; Park, J.W. RNA-seq transcriptome analysis in flounder cells to compare innate immune responses to low- and high-virulence viral hemorrhagic septicemis virus. Arch. Virol 2020, 166, 191–206. [Google Scholar] [CrossRef] [PubMed]
  66. Lopez-Vazquez, C.; Alonso, M.C.; Dopazo, C.P.; Bandín, I. In vivo study of viral haemorrhagic septicaemia virus and infectious pancreatic necrosis virus coexistence in Senegalese sole (Solea senegalensis). J. Fish Diseases 2017, 40, 1129–1139. [Google Scholar] [CrossRef] [PubMed]
  67. Pereiro, P.; Costa, M.M.; Díaz-Rosales, P.; Dios, S.; Figueras, A.; Novoa, B. The first characterization of two type I interferons in turbot (Scophtalmus maximus) reveals their differential role, expression pattern and gene induction. Dev. Comp. Immunol. 2014, 45, 233–244. [Google Scholar] [CrossRef]
  68. Wang, H.; Tang, X.; Sheng, X.; Xing, J.; Chi, H.; Zhan, W. Transcriptome analysis reveals temperature-dependent early immune response in flounder (Paralychthys olivaceus) after Hirame novirhabdovirus (HIRRV) infection. Fish Shellfish Immunol. 2020, 107, 367–378. [Google Scholar] [CrossRef]
  69. Olson, W.; Emmenegger, E.; Glenn, J.; Simchick, C.; Winton, J.R.; Goetz, F. Expression kinetics of key genes in the early innate immune response to Great Lakes viral hemorrhagic septicemia virus IVb infection in yellow perch (Perca flavescens). Dev. Comp. Immunol. 2013, 41, 11–19. [Google Scholar] [CrossRef]
  70. Moreno, P.; Lopez-Jimena, B.; Randelli, E.; Scapigliati, G.; Buonocore, F.; Garcia-Rosado, E.; Borrego, J.J.; Alonso, M.C. Immuno-related gene transcription and antibody response in nodavirus (RGNNV and SJNNV)-infected European sea bass (Dicentrarchus labrax L.). Fish Shellfish Immunol. 2018, 78, 270–278. [Google Scholar] [CrossRef]
  71. Scapigliati, G.; Buonocore, F.; Randelli, E.; Casani, D.; Meloni, S.; Zarletti, G.; Tiberi, M.; Pietretti, D.; Boschi, I.; Manchado, M.; et al. Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus. Fish Shellfish Immunol. 2009, 28, 303–311. [Google Scholar] [CrossRef]
  72. Wu, Y.C.; Chi, S.C. Persistence of betanodavirus in Barramundi brain (BB) cell line involves the induction of interferon response. Fish Shellfish Immunol. 2006, 21, 540–547. [Google Scholar] [CrossRef] [PubMed]
  73. Wu, Y.C.; Tsai, P.Y.; Chan, J.C.; Chi, S.C. Endogenous grouper and barramundi Mx proteins facilitated the clearance of betanodavirus RNA-dependent RNA polymerase. Dev. Comp. Immunol. 2016, 59, 110–120. [Google Scholar] [CrossRef] [PubMed]
  74. Thwaite, R.; Ji, J.; Torrealba, D.; Coll, J.; Sabés, M.; Villaverde, A.; Roher, N. Protein nanoparticles made of recombinant viral antigens: A promising biomaterial for oral delivery of fish prophylactics. Front. Immunol. 2018, 9, 1652. [Google Scholar] [CrossRef] [PubMed]
  75. Mugimba, K.K.; Lamkhannat, M.; Dubey, S.; Mutoloki, S.; Munang’andu, H.M.; Evensen, O. Tilapia lake virus downplays innate immune responses during early stage of infection in Nile tilapia (Oreochromis niloticus). Sci. Rep. 2020, 10, 20364. [Google Scholar] [CrossRef] [PubMed]
  76. Widziolek, M.; Janik, K.; Mojzesz, M.; Pooranachandran, N.; Adamek, M.; Pecio, A.; Surachetpong, W.; Levraud, J.P.; Boudinot, P.; Chadzinska, M.; et al. Type I interferon-dependent response of zebrafish larvae during tilapia lake virus (TiLV) infection. Dev. Comp. Immunol. 2021, 116, 103936. [Google Scholar] [CrossRef]
  77. Zhang, Y.; Wang, Y.; Gui, J. Identification and characterization of two homologues of interferon-stimulated gene ISG15 in crucian carp. Fish Shellfish Immunol. 2007, 23, 52–61. [Google Scholar] [CrossRef]
  78. Parreño, R.; Torres, S.; Almagro, L.; Bello-Perez, M.; Estepa, A.; Perez, L. Induction of viral interference by IPNV-carrier cells on target cells: A cell co-culture study. Fish Shellfish Immunol. 2016, 58, 483–489. [Google Scholar] [CrossRef] [Green Version]
  79. Poynter, S.J.; Leis, E.M.; DeWitte-Orr, S. In vitro transcribed dsRNA limits viral hemorrhagic septicemia virus (VHSV)-IVb infection in a novel fathead minnow (Pimephales promelas) skin cell line. Fish Shellfish Immunol. 2019, 86, 403–409. [Google Scholar] [CrossRef]
  80. Sun, C.; Liu, Y.; Hu, Y.; Fan, Q.; Yu, X.; Mao, H.; Hu, C. Gig1 and Gig2 homologs (CiGig1 and CiGig2) from grass carp (Ctenopharyngodon idella) display good antiviral activities in an IFN-independent pathway. Dev. Comp. Immunol. 2013, 41, 477–483. [Google Scholar] [CrossRef]
  81. Zhang, Y.-B.; Jiang, J.; Chen, Y.-D.; Zhu, R.; Shi, Y.; Zhang, Q.; Gui, J.-F. The innate immune response to grass carp hemorrhagic virus (GCHV) in cultured Carassius auratus blastulae (CAB) cells. Dev. Comp. Immunol. 2007, 31, 232–243. [Google Scholar] [CrossRef]
  82. Garcia, I.; Galiana, A.; Falco, A.; Estepa, A.; Perez, L. Characterization of an infectious pancreatic necrosis (IPN) virus carrier cell culture with resistance to superinfection with heterologous viruses. Vet. Microbiol. 2011, 149, 48–55. [Google Scholar] [CrossRef] [PubMed]
  83. Jurado, M.T.; Garcia-Valtanen, P.; Estepa, A.; Perez, L. Antiviral activity produced by an IPNV-carrier EPC cell culture confers resistance to VHSV infection. Vet. Microbiol. 2013, 166, 412–418. [Google Scholar] [CrossRef] [PubMed]
  84. Kavaliauskis, A.; Arnemo, M.; Kim, S.H.; Ulanova, L.; Speth, M.; Novoa, B.; Dios, S.; Evensen, O.; Griffiths, G.W.; Gjoen, T. Use of poly(I:C) stabilized with chitosan as a vaccine-adjuvant against viral hemorrhagic septicemia virus infection in zebrafish. Zebrafish 2015, 12, 421–431. [Google Scholar] [CrossRef] [PubMed]
  85. Kavaliauskis, A.; Arnemo, M.; Speth, M.; Lagos, L.; Rishovd, A.-L.; Estepa, A.; Griffiths, G.W.; Gjoen, T. Protective effect of a recombinant VHSV-G vaccine using poly(I:C) loaded nanoparticles as an adjuvant in zebrafish (Danio rerio) infection model. Dev. Comp. Immunol. 2016, 61, 248–257. [Google Scholar] [CrossRef]
  86. Du, X. Transcriptome profiling of spleen provides insights into the antiviral mechanism in Schizotorax prenanti after poly (I:C) challenge. Fish Shellfish Immunol. 2017, 62, 13–23. [Google Scholar] [CrossRef] [PubMed]
  87. Dai, Z.; Li, J.; Hu, C.; Wang, F.; Wang, B.; Shi, X.; Hou, Q.; Huang, W.; Lin, G. Transcriptome data analysis of grass carp (Ctenopharyngodon idella) infected by reoviruses provides insights into two immune-related genes. Fish Shellfish Immunol. 2017, 64, 68–77. [Google Scholar] [CrossRef]
  88. He, L.; Zhang, A.; Pei, Y.; Chu, P.; Li, Y.; Huang, R.; Liao, L.; Zhu, Z.; Wang, Y. Differences in responses of grass carp to different types of grass carp reovirus (GCRV) and the mechanism of hemorrhage revealed by transcriptome sequencing. BMC Genom. 2017, 18, 452. [Google Scholar] [CrossRef]
  89. Al-Hussinee, L.; Pham, P.H.; Russell, S.; Tubbs, L.; Tafalla, C.; Bols, N.C.; Dixon, B.; Lumsden, J.S. Temporary protection of rainbow trout gill epithelial cells from infection with viral haemorrhagic septicaemia virus IVb. J. Fish Diseases 2016, 39, 1099–1112. [Google Scholar] [CrossRef]
  90. Jensen, I.; Larsen, R.; Robertsen, B. An antiviral state induced in Chinook salmon embryo cells (CHSE-214) by transfection with the double-stranded RNA in poly I:C. Fish Shellfish Immunol. 2002, 13, 367–378. [Google Scholar] [CrossRef]
  91. Monjo, A.L.; Poynter, S.J.; DeWitte-Orr, S. CHSE-214: A model for studying extracellular dsRNA sensing in vitro. Fish Shellfish Immunol. 2017, 68, 266–271. [Google Scholar] [CrossRef]
  92. Fierro-Castro, C.; Barrioluengo, L.; López-Fierro, P.; Razquin, B.E.; Villena, A.J. Fish cell cultures as in vitro models of imflammatory responses elicited by immunostimulants. Fish Shellfish Immunol. 2013, 35, 979–987. [Google Scholar] [CrossRef] [PubMed]
  93. Poynter, S.J.; DeWitte-Orr, S. Length-dependent innate antiviral effects of double-stranded RNA in the rainbow trout (Onchorhyncus mykiss) cell line, RTG-2. Fish Shellfish Immunol. 2015, 46, 557–565. [Google Scholar] [CrossRef] [PubMed]
  94. Poynter, S.J.; DeWitte-Orr, S. Understanding viral dsRNA-mediated innate immune responses at the cellular level using a rainbow trout model. Front. Immunol. 2018, 9, 829. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  95. Villalba, M.; Pérez, V.; Herrera, L.; Stepke, C.; Maldonado, N.; Freddericksen, F.; Yáñez, A.; Olavarría, V.H. Infectious pancreatic necrosis virus infection of fish cell lines: Preliminary analysis of gene expressions related to extracellular matrix remodeling and immunity. Vet. Immunol. Immunopathol. 2017, 193–194, 10–17. [Google Scholar] [CrossRef] [PubMed]
  96. DeWitte-Orr, S.; Leong, J.C.; Bols, N.C. Induction of antiviral genes, Mx and vig-1, by dsRNA and Chum salmon reovirus in rainbow trout monocyte/macrophage and fibroblasts cell lines. Fish Shellfish Immunol. 2007, 23, 670–682. [Google Scholar] [CrossRef]
  97. Nombela, I.; Carrion, A.; Puente-Marin, S.; Chico, V.; Mercado, L.; Perez, L.; Coll, J.; Ortega-Villaizan, M. Infectious pancreatic necrosis virus triggers antiviral immune response in rainbow trout red blood cells, despite not being infective. F1000 Res. 2017, 6, 1968. [Google Scholar] [CrossRef]
  98. Kim, H.J.; Park, J.S.; Choi, M.C.; Kwon, S.R. Comparison of the efficacy of poly(I:C) immunization with live vaccine and formalin-killed vaccine against viral hemorrhagic septicemia virus (VHSV) in olive flounder (Paralichtys olivaceus). Fish Shellfish Immunol. 2016, 48, 206–211. [Google Scholar] [CrossRef]
  99. Rosaeg, M.V.; Lund, M.; Nyman, I.B.; Markussen, T.; Asperhaug, V.; Sindre, H.; Dahle, M.K.; Rimstad, E. Immunological interactions between Piscine orthoreovirus and Salmonid alphavirus infections in Atlantic salmon. Fish Shellfish Immunol. 2017, 64, 308–319. [Google Scholar] [CrossRef]
  100. Wessel, O.; Olsen, C.M.; Rimstad, E.; Dahle, M.K. Piscine orthoreovirus (PRV) replicates in Atlantic salmon (Salmo salar L.) erythrocytes ex vivo. Vet. Res. 2015, 46, 26. [Google Scholar] [CrossRef] [Green Version]
  101. Lockhart, K.; McBeath, A.J.A.; Collet, B.; Snow, M.; Ellis, A.E. Expression of Mx mRNA following infection with IPNV is greater in IPN-susceptible Atlantic salmon post-smolts than in IPN-resistant Atlantic salmon parr. Fish Shellfish Immunol. 2007, 22, 151–156. [Google Scholar] [CrossRef]
  102. Reyes-Lopez, F.; Romero, J.S.; Vallejos-Vidal, E.; Reyes-Cerpa, S.; Sandino, A.M.; Tort, L.; MacKenzie, S.; Imarai, M. Differential immune gene expression profiles in susceptible and resistant full-sibling families of Atlantic salmon (Salmo salar) challenged with infectious pancreatic necrosis virus (IPNV). Dev. Comp. Immunol. 2015, 53, 210–221. [Google Scholar] [CrossRef] [PubMed]
  103. Robledo, D.; Taggart, J.B.; Ireland, J.H.; McAndrew, B.J.; Starkey, W.G.; Haley, C.S.; Hamilton, A.; Guy, D.R.; Mota-Velasco, J.C.; Gheyas, A.A.; et al. Gene expression comparison of resistant and susceptible Atlantic salmon fry challenged with Infectious Pancreatic Necrosis virus reveals a marked contrast in immune response. BMC Genom. 2016, 17, 279. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  104. Timmerhaus, G.; Krasnov, A.; Nilsen, P.; Alarcon, M.; Afanasyev, S.; Rode, M.; Takle, H.; Jorgensen, S.V. Transcriptome profiling of immune responses to cardiomyopathy syndrome (CMS) in Atlantic samon. BMC Genom. 2011, 12, 459. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  105. Milev-Milovanovic, I.; Majji, S.; Thodima, V.; Deng, Y.; Hanson, L.; Arnizaut, A.; Waldbieser, G.; Chinchar, G. Identification and expression analyses of poly (I:C)-stimulated genes in channel catfish (Ictalurus punctatus). Fish Shellfish Immunol. 2009, 26, 811–820. [Google Scholar] [CrossRef] [PubMed]
  106. Plant, K.P.; Harbottle, H.; Thune, R.L. Poly I:C induces an antiviral state against Ictalurid Herpesvirus 1 and Mx1 transcription in the channel catfish (Ictalurus punctatus). Dev. Comp. Immunol. 2005, 29, 627–635. [Google Scholar] [CrossRef] [PubMed]
  107. Jung, M.-H.; Jung, S. Innate immune response against rock bream iridovirus (RBIV) infection in rock bream (Oplegnatus fasciatus) following poly (I:C) administration. Fish Shellfish Immunol. 2017, 71, 171–176. [Google Scholar] [CrossRef] [PubMed]
  108. Mu, Y.; Li, M.; Ding, F.; Ding, Y.; Ao, J.; Hu, S.; Chen, X. De novo characterization of the spleen transcriptome of the large yellow croaker (Pseudosciaena crocea) and analysis of the immune relevant genes and pathways involved in the antiviral response. PLoS ONE 2014, 9, e97471. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  109. Pakingking, R.; Okinaka, Y.; Mori, K.; Arimoto, M.; Muroga, K.; Nakai, T. In vivo and in vitro analysis of the resistance against viral haemorrhagic septicaemia virus in Japanese flounder (Paralichtys olivaceus) precedingly infected with aquabirnavirus. Fish Shellfish Immunol. 2004, 17, 1–11. [Google Scholar] [CrossRef]
  110. Chang, C.J. Immune sensing of DNA and strategies for fish DNA vaccine development. Fish Shellfish Immunol. 2020, 101, 252–260. [Google Scholar] [CrossRef]
  111. Carballo, C.; Castro, D.; Borrego, J.J.; Manchado, M. Gene expression profiles associated with lymphocystis disease virus (LCDV) in experimentally infected Senegalese sole (Solea senegalensis). Fish Shellfish Immunol. 2017, 66, 129–139. [Google Scholar] [CrossRef]
  112. Zhang, J.; Hu, Y.; Xiao, Z.; Sun, L. Megalocytivirus-induced proteins of turbot (Scophthalmus maximus): Identification and antiviral potential. J. Proteomics 2013, 91, 430–443. [Google Scholar] [CrossRef] [PubMed]
  113. Cordero, H.; Cuesta, A.; Meseguer, J.; Esteban, M.A. Characterization of the gilthead (Sparus aurata L.) immune response under a natural lymphocystis disease virus outbreak. J. Fish Dis. 2016, 39, 1476. [Google Scholar] [CrossRef] [PubMed]
  114. Li, L.; Chen, S.N.; Laghari, Z.A.; Hou, H.J.; Hou, J.; Huang, L.; Nan, L.; Nie, P. Myxovirus resistance (Mx) gene and its differential expression regulated by three type I and two type II IFNs in mandarin fish, Siniperca chuatsi. Dev. Comp. Immunol. 2020, 105, 103604. [Google Scholar] [CrossRef] [PubMed]
  115. Xia, S.; Wang, H.; Hong, X.; Lu, J.; Xu, D.; Jiang, Y.; Lu, L. Identification and characterization of a type I interferon induced by cyprinid herpesvirus 2 infection in crucian carp Carassius auratus gibelio. Fish Shellfish Immunol. 2018, 76, 35–40. [Google Scholar] [CrossRef]
  116. Hwang, J.A.; Kim, J.E.; Kim, H.S.; Lee, J.H. Immune response to koi herpesvirus (KHV) of koi and koi × red common carp (Cyprinus carpio). Dev. Reprod. 2017, 2017, 361–370. [Google Scholar] [CrossRef] [Green Version]
  117. Pietretti, D.; Wiegertjes, G.F. Ligand specificities of Toll-like receptors in fish: Indications from infection studies. Dev. Comp. Immunol. 2014, 43, 205–222. [Google Scholar] [CrossRef]
  118. Zhang, B.C.; Zhang, J.; Xiao, Z.Z.; Sun, L. Rock bream (Oplegnathus fasciatus) viperin is a virus-responsive protein that modulates innate immunity and promotes resistance against megalocytivirus infection. Dev. Comp. Immunol. 2014, 45, 35–42. [Google Scholar] [CrossRef]
  119. Zhang, Y.; Lv, S.; Zheng, J.; Huang, X.; Huang, Y.; Qin, Q. Grouper viperin acts as a crucial antiviral molecule against iridovirus. Fish Shellfish Immunol. 2019, 86, 1026–1034. [Google Scholar] [CrossRef]
  120. Gjessing, M.C.; Krasnov, A.; Timmerhaus, G.; Brun, S.; Afanasyev, S.; Dale, O.B.; Dahle, M.K. The Atlantic salmon gill transcriptome response in a natural outbreak of salmon gill pox virus infection reveals new biomarkers of gill pathology and suppression of mucosal defense. Front Immunol. 2020, 11, 2154. [Google Scholar] [CrossRef]
  121. Rakus, K.L.; Irnazarow, I.; Adamek, M.; Palmeira, L.; Kawana, Y.; Hirono, I. Gene expression analysis of common carp (Cyprinus carpio L.) lines during Cyprinid herpesvirus 3 infection yields insights into differential immune responses. Dev. Comp. Immunol. 2012, 37, 65–67. [Google Scholar] [CrossRef]
  122. Kileng, O.; Brundtland, M.I.; Robertsen, B. Infectious salmon anemia virus is a powerful inducer of key genes of the type I interferon system of Atlantic salmon, but is not inhibited by interferon. Fish Shellfish Immunol. 2007, 23, 378–389. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  123. Tafalla, C.; Chico, V.; Perez, L.; Coll, J.M.; Estepa, A. In vitro and in vivo differential expression of rainbow trout (Oncorhynchus mykiss) Mx isoforms in response to viral haemorrhagic septicaemia virus (VHSV) G gene, poly I:C and VHSV. Fish Shellfish Immunol. 2007, 23, 210–221. [Google Scholar] [CrossRef] [PubMed]
  124. Zhou, X.; Lu, L.; Li, Z.; Zhang, C.; Chen, D.; Li, S. Temperature effects on SVCV propagation and the related IFN response in zebrafish. Aquaculture 2021, 533, 736084. [Google Scholar] [CrossRef]
  125. Liu, R.; Hu, X.; Lu, A.; Song, Y.; Lian, Z.; Sun, J.; Sung, Y.Y. Proteomic profiling of zebrafish challenged by spring viremia of carp virus provides insight into skin antiviral response. Zebrafish 2020, 17, 91–103. [Google Scholar] [CrossRef] [PubMed]
  126. Cano, I.; Collet, B.; Pereira, C.; Paley, R.; van Aerle, R.; Stone, D.; Taylor, N.G.H. In vivo virulence of viral haemorrhagic septicaemia virus (VHSV) in rainbow trout Oncorhynchus mykiss correlates inversely with in vitro Mx gene expression. Vet. Microbiol. 2016, 187, 31–40. [Google Scholar] [CrossRef] [PubMed]
  127. García-Rosado, E.; Alonso, M.; Bejar, J.; Manchado, M.; Cano, I.; Borrego, J.J. Expression analysis of Mx protein and evaluation of its antiviral activity against sole aquabirnavirus in SAF-1 and TV-1 cell lines. Vet. Immunol. Immunopathol. 2008, 121, 123–129. [Google Scholar] [CrossRef] [PubMed]
  128. Chen, Y.-M.; Su, Y.-L.; Shie, P.-S.; Huang, S.-L.; Yang, H.-L.; Chen, T.-Y. Grouper Mx confers resistance to nodavirus and interacts with coat protein. Dev. Comp. Immunol. 2008, 32, 825–836. [Google Scholar] [CrossRef]
  129. Kim, M.S.; Shin, M.J.; Kim, K.H. Increase of viral hemorrhagic septicemia virus growth by knockout of IRF9 gene in Epithelioma papulosum cyprini cells. Fish Shellfish Immunol. 2018, 83, 443–448. [Google Scholar] [CrossRef]
  130. Kim, M.S.; Kim, K.H. Effect of CRISPR/Cas9-mediated knockout of either Mx1 or ISG15 gene in EPC cells on resistance against VHSV infection. Fish Shellfish Immunol. 2019, 93, 1041–1046. [Google Scholar] [CrossRef]
  131. Yu, G.; Liu, X.; Tang, J.; Xu, C.; Ouyang, G.; Xiao, W. Neddylation facilitates the antiviral response in zebrafish. Front. Immunol. 2019, 10, 1432. [Google Scholar] [CrossRef] [Green Version]
  132. Avunje, S.; Jung, S. Poly (I:C) and imiquimod induced immune responses and their effects on the survival of olive flounder (Paralichtys olivaceus) from viral haemorrhagic septicaemia. Fish Shellfish Immunol. 2017, 71, 338–345. [Google Scholar] [CrossRef] [PubMed]
  133. Garcia-Valtanen, P.; Martinez-Lopez, A.; Lopez-Muñoz, A.; Bello-Perez, M.; Medina-Gali, R.M.; Ortega-Villaizan, M.; Varela, M.; Figueras, A.; Mulero, V.; Novoa, B.; et al. Zebra fish lacking adaptive immunity acquire an antiviral alert state characterized by upregulated gene expression of apoptosis, multigene families, and interferon-related genes. Front. Immunol. 2017, 8, 121. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  134. Gao, F.X.; Wang, Y.; Zhang, Q.-Y.; Mou, C.Y.; Li, Z.; Deng, Y.S.; Zhou, L.; Gui, J.-F. Distinct herpesvirus resistances and immune responses of three gynogenetic clones of gibel carp revealed by comprehensive transcriptomes. BMC Genom. 2017, 18, 561. [Google Scholar] [CrossRef] [PubMed]
  135. Mou, C.Y.; Wang, Y.; Zhang, Q.-Y.; Gao, F.X.; Li, Z.; Tong, J.F.; Zhou, L.; Gui, J.F. Differential interferon system gene expression profiles in susceptible and resistant gynogenetic clones of gibel carp challenged with herpesvirus CaHV. Dev. Comp. Immunol. 2018, 86, 52–64. [Google Scholar] [CrossRef]
  136. Xiao, J.; Fu, Y.; Zhou, W.; Peng, L.; Xiao, J.; Liu, S.; Feng, H. Establishment of fin cell lines and their use to study the immune gene expression in cyprinid fishes with different ploidy in rhabdovirus infection. Dev. Comp. Immunol. 2018, 88, 55–64. [Google Scholar] [CrossRef] [PubMed]
  137. Netea, M.G.; Joosten, L.A.B.; Latz, E.; Mills, K.H.G.; Natoli, G.; Stunnenberg, H.G.; O’Neill, L.A.J.; Xavier, R.J. Trained immunity: A program of innate immune memory in health and disease. Science 2016, 352, aaf1098. [Google Scholar] [CrossRef] [PubMed] [Green Version]
Table
Table 1. Viruses cited in this work.
Table 1. Viruses cited in this work.
ssRNANameAcronymMain host
AmnoonviridaeTilapia lake virusTiLVTilapia
NodaviridaeNervous necrosis virusNNVPerciformes
OrthomyxoviridaeInfectious salmon anemia virusISAVSalmonid
RhabdoviridaeHirame novirhabdovirusHIRRVSalmonid
Infectious hematopoietic necrosis virusIHNV 1Salmonid
Spring viremia of carp virusSVCVCyprinid
Viral hemorrhagic septicemia virusVHSV 2Salmonid
TogaviridaeSalmonid alphavirusSAVSalmonid
dsRNA
BirnaviridaeInfectious pancreatic necrosis virusIPNVSalmonid
ReoviridaeChum salmon reovirusCSVSalmonid
Grass carp reovirusGCRVCyprinid
Piscine reovirusPRVSalmonid
TotiviridaePiscine myocarditis virusPMCVSalmonid
dsDNA
HerpesviridaeChannel catfish virus (Ictalurid herpesvirus)CCVIctalurid
Cyprinid herpesvirusCyHVCyprinid
IridoviridaeInfectious spleen and kidney necrosis virusISKNVS. chuatsi
Lymphocystis disease virusLCDVSea bream, sole
Rock bream iridovirusRBIVO. fasciatus
1 Salmonid novirhabdovirus; 2 Piscine novirhabdovirus.
Table
Table 2. Innate immune genes ifn, mx, isg15, vig/viperin, and gig stimulation by viruses of teleost fish.
Table 2. Innate immune genes ifn, mx, isg15, vig/viperin, and gig stimulation by viruses of teleost fish.
ssRNACYPRINIFORMESSALMONIFORMESOTHERS
TiLV ifn, mx
NNVmx ifn, mx, isg15
ISAV ifn, mx, isg15, gig
IHNVifnifn, mx, vig
SVCVifn, mx, vig, isg15, gig
VHSVifn, mx, vigifn, mx, isg15ifn, mx
SAV ifn, mx, vig, gig
dsRNA/polyICCYPRINIFORMESSALMONIFORMESOTHERS
poly I:Cifn, mx, vig, gigifn, mx, vigifn, mx, isg15
IPNVmxifn, mx, gigifn, mx, isg15
CSV mx
GCRVifn, mx, isg15
PRV ifn, mx, isg15, vig
PMCV vig
dsDNACYPRINIFORMESSALMONIFORMESOTHERS
CCV mx
ISKNV mx
LCDV mx
RBIV mx, isg15, vig
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Ortega-Villaizan, M.d.M.; Chico, V.; Perez, L. Fish Innate Immune Response to Viral Infection—An Overview of Five Major Antiviral Genes. Viruses 2022, 14, 1546. https://doi.org/10.3390/v14071546

AMA Style

Ortega-Villaizan MdM, Chico V, Perez L. Fish Innate Immune Response to Viral Infection—An Overview of Five Major Antiviral Genes. Viruses. 2022; 14(7):1546. https://doi.org/10.3390/v14071546

Chicago/Turabian Style

Ortega-Villaizan, Maria del Mar, Veronica Chico, and Luis Perez. 2022. "Fish Innate Immune Response to Viral Infection—An Overview of Five Major Antiviral Genes" Viruses 14, no. 7: 1546. https://doi.org/10.3390/v14071546

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop