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Article

Induction of Hepatitis E Virus Anti-ORF3 Antibodies from Systemic Administration of a Muscle-Specific Adeno-Associated Virus (AAV) Vector

1
Schaller Research Group, Department of Infectious Diseases, Virology, University Hospital Heidelberg, Center for Integrative Infectious Diseases Research (CIID), 61920 Heidelberg, Germany
2
Department of Infectious Diseases, Virology, University Hospital Heidelberg, Cluster of Excellence CellNetworks, BioQuant, Center for Integrative Infectious Diseases Research (CIID), 69120 Heidelberg, Germany
3
Department of Molecular and Medical Virology, Ruhr-University Bochum, 44801 Bochum, Germany
4
German Center for Infection Research (DZIF), External Partner Site Bochum, 44801 Bochum, Germany
5
German Center for Infection Research (DZIF), Partner Site Heidelberg, 69120 Heidelberg, Germany
6
German Center for Cardiovascular Research (DZHK), Partner Site Heidelberg, 69120 Heidelberg, Germany
*
Authors to whom correspondence should be addressed.
Current address: DiNAQOR, Vector Development Core, 8952 Schlieren, Switzerland.
These authors contributed equally to this work.
Academic Editor: Stefan Weger
Viruses 2022, 14(2), 266; https://doi.org/10.3390/v14020266
Received: 31 December 2021 / Revised: 23 January 2022 / Accepted: 24 January 2022 / Published: 27 January 2022
(This article belongs to the Special Issue Novel Developments and Perspectives in Viral Vector Technology)
The hepatitis E virus (HEV) is a major global health problem, leading to large outbreaks in the developing world and chronic infections in the developed world. HEV is a non-enveloped virus, which circulates in the blood in a quasi-enveloped form. The quasi-envelope protects HEV particles from neutralising anti-capsid antibodies in the serum; however, most vaccine approaches are designed to induce an immune response against the HEV capsid. In this study, we explored systemic in vivo administration of a novel synthetic and myotropic Adeno-associated virus vector (AAVMYO3) to express the small HEV phosphoprotein ORF3 (found on quasi-enveloped HEV) in the musculature of mice, resulting in the robust and dose-dependent formation of anti-ORF3 antibodies. Neutralisation assays using the serum of ORF3 AAV-transduced mice showed a modest inhibitory effect on the infection of quasi-enveloped HEV in vivo, comparable to previously characterised anti-ORF3 antibodies used as a control. The novel AAVMYO3 capsid used in this study can serve as a versatile platform for the continued development of vector-based vaccines against HEV and other infectious agents, which could complement traditional vaccines akin to the current positive experience with SARS-CoV-2. View Full-Text
Keywords: adeno-associated virus; AAV; hepatitis E virus; HEV; vector-based vaccine; neutralisation adeno-associated virus; AAV; hepatitis E virus; HEV; vector-based vaccine; neutralisation
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MDPI and ACS Style

Maurer, L.; El Andari, J.; Rapti, K.; Spreyer, L.; Steinmann, E.; Grimm, D.; Dao Thi, V.L. Induction of Hepatitis E Virus Anti-ORF3 Antibodies from Systemic Administration of a Muscle-Specific Adeno-Associated Virus (AAV) Vector. Viruses 2022, 14, 266. https://doi.org/10.3390/v14020266

AMA Style

Maurer L, El Andari J, Rapti K, Spreyer L, Steinmann E, Grimm D, Dao Thi VL. Induction of Hepatitis E Virus Anti-ORF3 Antibodies from Systemic Administration of a Muscle-Specific Adeno-Associated Virus (AAV) Vector. Viruses. 2022; 14(2):266. https://doi.org/10.3390/v14020266

Chicago/Turabian Style

Maurer, Lars, Jihad El Andari, Kleopatra Rapti, Laura Spreyer, Eike Steinmann, Dirk Grimm, and Viet L. Dao Thi. 2022. "Induction of Hepatitis E Virus Anti-ORF3 Antibodies from Systemic Administration of a Muscle-Specific Adeno-Associated Virus (AAV) Vector" Viruses 14, no. 2: 266. https://doi.org/10.3390/v14020266

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