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Communication

SARS-CoV-2 Spike Expression at the Surface of Infected Primary Human Airway Epithelial Cells

1
Centre de Recherche du CHUM (CRCHUM), Montréal, QC H2X 0A9, Canada
2
Département de Médicine, Université de Montréal, Montréal, QC H2X 0A9, Canada
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Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montréal, QC H2X 0A9, Canada
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Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada
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Laboratoire de Santé Publique du Québec, Institut Nationale de Santé Publique du Québec, Sainte-Anne-de-Bellevue, QC H9X 3R5, Canada
*
Author to whom correspondence should be addressed.
Academic Editor: Caijun Sun
Viruses 2022, 14(1), 5; https://doi.org/10.3390/v14010005
Received: 1 December 2021 / Revised: 14 December 2021 / Accepted: 16 December 2021 / Published: 21 December 2021
(This article belongs to the Section SARS-CoV-2 and COVID-19)
Different serological assays were rapidly generated to study humoral responses against the SARS-CoV-2 Spike glycoprotein. Due to the intrinsic difficulty of working with SARS-CoV-2 authentic virus, most serological assays use recombinant forms of the Spike glycoprotein or its receptor binding domain (RBD). Cell-based assays expressing different forms of the Spike, as well as pseudoviral assays, are also widely used. To evaluate whether these assays recapitulate findings generated when the Spike is expressed in its physiological context (at the surface of the infected primary cells), we developed an intracellular staining against the SARS-CoV-2 nucleocapsid (N) to distinguish infected from uninfected cells. Human airway epithelial cells (pAECs) were infected with authentic SARS-CoV-2 D614G or Alpha variants. We observed robust cell-surface expression of the SARS-CoV-2 Spike at the surface of the infected pAECs using the conformational-independent anti-S2 CV3-25 antibody. The infected cells were also readily recognized by plasma from convalescent and vaccinated individuals and correlated with several serological assays. This suggests that the antigenicity of the Spike present at the surface of the infected primary cells is maintained in serological assays involving expression of the native full-length Spike. View Full-Text
Keywords: COVID-19; SARS-CoV-2; spike glycoproteins; nucleocapsid; authentic virus; human primary airway epithelial cells; convalescent plasma; mRNA vaccine; neutralization; antibody-dependent cellular cytotoxicity (ADCC) COVID-19; SARS-CoV-2; spike glycoproteins; nucleocapsid; authentic virus; human primary airway epithelial cells; convalescent plasma; mRNA vaccine; neutralization; antibody-dependent cellular cytotoxicity (ADCC)
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MDPI and ACS Style

Ding, S.; Adam, D.; Beaudoin-Bussières, G.; Tauzin, A.; Gong, S.Y.; Gasser, R.; Laumaea, A.; Anand, S.P.; Privé, A.; Bourassa, C.; Medjahed, H.; Prévost, J.; Charest, H.; Richard, J.; Brochiero, E.; Finzi, A. SARS-CoV-2 Spike Expression at the Surface of Infected Primary Human Airway Epithelial Cells. Viruses 2022, 14, 5. https://doi.org/10.3390/v14010005

AMA Style

Ding S, Adam D, Beaudoin-Bussières G, Tauzin A, Gong SY, Gasser R, Laumaea A, Anand SP, Privé A, Bourassa C, Medjahed H, Prévost J, Charest H, Richard J, Brochiero E, Finzi A. SARS-CoV-2 Spike Expression at the Surface of Infected Primary Human Airway Epithelial Cells. Viruses. 2022; 14(1):5. https://doi.org/10.3390/v14010005

Chicago/Turabian Style

Ding, Shilei, Damien Adam, Guillaume Beaudoin-Bussières, Alexandra Tauzin, Shang Y. Gong, Romain Gasser, Annemarie Laumaea, Sai P. Anand, Anik Privé, Catherine Bourassa, Halima Medjahed, Jérémie Prévost, Hugues Charest, Jonathan Richard, Emmanuelle Brochiero, and Andrés Finzi. 2022. "SARS-CoV-2 Spike Expression at the Surface of Infected Primary Human Airway Epithelial Cells" Viruses 14, no. 1: 5. https://doi.org/10.3390/v14010005

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