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Article

Comparison of the Virome of Quarantined Sugarcane Varieties and the Virome of Grasses Growing near the Quarantine Station

1
CIRAD, UMR PHIM, 34090 Montpellier, France
2
PHIM Plant Health Institute, Univ Montpellier, CIRAD, INRAE, Institut Agro, IRD, 34090 Montpellier, France
3
Tour du Valat, Research Institute for the Conservation of Mediterranean Wetlands, 13200 Arles, France
4
Institut Méditerranéen de Biodiversité et Ecologie, UMR CNRS-IRD, Avignon Université, Aix-Marseille Université, IUT d’Avignon, 337 chemin des Meinajariés, Site Agroparc BP 61207, 84911 Avignon, France
*
Author to whom correspondence should be addressed.
Academic Editor: Henryk Czosnek
Viruses 2021, 13(5), 922; https://doi.org/10.3390/v13050922
Received: 13 April 2021 / Revised: 28 April 2021 / Accepted: 10 May 2021 / Published: 16 May 2021
(This article belongs to the Special Issue Plant Virus Surveillance and Metagenomics)
Visacane is a sugarcane quarantine station located in the South of France, far away from sugarcane growing areas. Visacane imports up to 100 sugarcane varieties per year, using safe control and confinement measures of plants and their wastes to prevent any risk of pathogen spread outside of the facilities. Viruses hosted by the imported material are either known or unknown to cause disease in cultivated sugarcane. Poaceae viruses occurring in plants surrounding the quarantine glasshouse are currently unknown. These viruses could be considered as a source of new sugarcane infections and potentially cause new sugarcane diseases in cases of confinement barrier failure. The aim of this study was to compare the plant virome inside and outside of the quarantine station to identify potential confinement failures and risks of cross infections. Leaves from quarantined sugarcane varieties and from wild Poaceae growing near the quarantine were collected and processed by a metagenomics approach based on virion-associated nucleic acids extraction and library preparation for Illumina sequencing. While viruses belonging to the same virus genus or family were identified in the sugarcane quarantine and its surroundings, no virus species was detected in both environments. Based on the data obtained in this study, no virus movement between quarantined sugarcane and nearby grassland has occurred so far, and the confinement procedures of Visacane appear to be properly implemented. View Full-Text
Keywords: viral metagenomics; plant quarantine; sugarcane viral metagenomics; plant quarantine; sugarcane
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MDPI and ACS Style

Daugrois, J.H.; Filloux, D.; Julian, C.; Claude, L.; Ferdinand, R.; Fernandez, E.; Fontes, H.; Rott, P.C.; Roumagnac, P. Comparison of the Virome of Quarantined Sugarcane Varieties and the Virome of Grasses Growing near the Quarantine Station. Viruses 2021, 13, 922. https://doi.org/10.3390/v13050922

AMA Style

Daugrois JH, Filloux D, Julian C, Claude L, Ferdinand R, Fernandez E, Fontes H, Rott PC, Roumagnac P. Comparison of the Virome of Quarantined Sugarcane Varieties and the Virome of Grasses Growing near the Quarantine Station. Viruses. 2021; 13(5):922. https://doi.org/10.3390/v13050922

Chicago/Turabian Style

Daugrois, Jean H., Denis Filloux, Charlotte Julian, Lisa Claude, Romain Ferdinand, Emmanuel Fernandez, Hugo Fontes, Philippe C. Rott, and Philippe Roumagnac. 2021. "Comparison of the Virome of Quarantined Sugarcane Varieties and the Virome of Grasses Growing near the Quarantine Station" Viruses 13, no. 5: 922. https://doi.org/10.3390/v13050922

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