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Article

Global Metabolic Profiling of Baculovirus Infection in Silkworm Hemolymph Shows the Importance of Amino-Acid Metabolism

1
Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China
2
Insect Molecular Genetics and Biotechnology, National Centre for Scientific Research Demokritos, Institute of Biosciences and Applications, 15310 Athens, Greece
*
Authors to whom correspondence should be addressed.
The authors contributed equally to the manuscript.
Academic Editor: A. Lorena Passarelli
Viruses 2021, 13(5), 841; https://doi.org/10.3390/v13050841
Received: 14 April 2021 / Revised: 28 April 2021 / Accepted: 1 May 2021 / Published: 6 May 2021
(This article belongs to the Section Insect Viruses)
Viruses rely on host cell metabolism to provide the necessary energy and biosynthetic precursors for successful viral replication. Infection of the silkworm, Bombyx mori, by Bombyx mori nucleopolyhedrovirus (BmNPV), has been studied extensively in the past to unravel interactions between baculoviruses and their lepidopteran hosts. To understand the interaction between the host metabolic responses and BmNPV infection, we analyzed global metabolic changes associated with BmNPV infection in silkworm hemolymph. Our metabolic profiling data suggests that amino acid metabolism is strikingly altered during a time course of BmNPV infection. Amino acid consumption is increased during BmNPV infection at 24 h post infection (hpi), but their abundance recovered at 72 hpi. Central carbon metabolism, on the other hand, particularly glycolysis and glutaminolysis, did not show obvious changes during BmNPV infection. Pharmacologically inhibiting the glycolytic pathway and glutaminolysis also failed to reduce BmNPV replication, revealing that glycolysis and glutaminolysis are not essential during BmNPV infection. This study reveals a unique amino acid utilization process that is implemented during BmNPV infection. Our metabolomic analysis of BmNPV-infected silkworm provides insights as to how baculoviruses induce alterations in host metabolism during systemic infection. View Full-Text
Keywords: baculoviruses; Bombyx mori; metabolome; BmNPV; amino acid baculoviruses; Bombyx mori; metabolome; BmNPV; amino acid
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MDPI and ACS Style

Feng, M.; Fei, S.; Xia, J.; Zhang, M.; Wu, H.; Swevers, L.; Sun, J. Global Metabolic Profiling of Baculovirus Infection in Silkworm Hemolymph Shows the Importance of Amino-Acid Metabolism. Viruses 2021, 13, 841. https://doi.org/10.3390/v13050841

AMA Style

Feng M, Fei S, Xia J, Zhang M, Wu H, Swevers L, Sun J. Global Metabolic Profiling of Baculovirus Infection in Silkworm Hemolymph Shows the Importance of Amino-Acid Metabolism. Viruses. 2021; 13(5):841. https://doi.org/10.3390/v13050841

Chicago/Turabian Style

Feng, Min, Shigang Fei, Junming Xia, Mengmeng Zhang, Hongyun Wu, Luc Swevers, and Jingchen Sun. 2021. "Global Metabolic Profiling of Baculovirus Infection in Silkworm Hemolymph Shows the Importance of Amino-Acid Metabolism" Viruses 13, no. 5: 841. https://doi.org/10.3390/v13050841

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