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Article

Superiority of MALDI-TOF Mass Spectrometry over Real-Time PCR for SARS-CoV-2 RNA Detection

1
Department of Molecular Diagnostics, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Abrahama 58, 80-307 Gdansk, Poland
2
Laboratory of Molecular Biology, Medical University of Gdansk, Marii Skłodowskiej-Curie 3A, 80-210 Gdansk, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Gregory Tannock
Viruses 2021, 13(5), 730; https://doi.org/10.3390/v13050730
Received: 25 March 2021 / Revised: 16 April 2021 / Accepted: 19 April 2021 / Published: 22 April 2021
(This article belongs to the Special Issue Strategies for Preventing Viral Epidemics and Pandemics)
At present, the RT-PCR test remains the gold standard for early diagnosis of SARS-CoV-2. Nevertheless, there is growing evidence demonstrating that this technique may generate false-negative results. Here, we aimed to compare the new mass spectrometry-based assay MassARRAY® SARS-CoV-2 Panel with the RT-PCR diagnostic test approved for clinical use. The study group consisted of 168 suspected patients with symptoms of a respiratory infection. After simultaneous analysis by RT-PCR and mass spectrometry methods, we obtained discordant results for 17 samples (10.12%). Within fifteen samples officially reported as presumptive positive, 13 were positive according to the MS-based assay. Moreover, four samples reported by the officially approved RT-PCR as negative were positive in at least one MS assay. We have successfully demonstrated superior sensitivity of the MS-based assay in SARS-CoV-2 detection, showing that MALDI-TOF MS seems to be ideal for the detection as well as discrimination of mutations within the viral genome. View Full-Text
Keywords: SARS-CoV-2; COVID-19; RT-PCR; mass spectrometry; epidemic; pandemic; diagnosis SARS-CoV-2; COVID-19; RT-PCR; mass spectrometry; epidemic; pandemic; diagnosis
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MDPI and ACS Style

Rybicka, M.; Miłosz, E.; Bielawski, K.P. Superiority of MALDI-TOF Mass Spectrometry over Real-Time PCR for SARS-CoV-2 RNA Detection. Viruses 2021, 13, 730. https://doi.org/10.3390/v13050730

AMA Style

Rybicka M, Miłosz E, Bielawski KP. Superiority of MALDI-TOF Mass Spectrometry over Real-Time PCR for SARS-CoV-2 RNA Detection. Viruses. 2021; 13(5):730. https://doi.org/10.3390/v13050730

Chicago/Turabian Style

Rybicka, Magda, Ewa Miłosz, and Krzysztof P. Bielawski 2021. "Superiority of MALDI-TOF Mass Spectrometry over Real-Time PCR for SARS-CoV-2 RNA Detection" Viruses 13, no. 5: 730. https://doi.org/10.3390/v13050730

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