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Establishment of a Genetically Confirmed Breeding Colony of Mastomys natalensis from Wild-Caught Founders from West Africa
Article

Mastomys natalensis Has a Cellular Immune Response Profile Distinct from Laboratory Mice

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Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, National Institute of Health, Hamilton, MT 59840, USA
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Research Technologies Branch, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT 59840, USA
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Rocky Mountain Veterinary Branch Division of Intramural Research, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, National Institute of Health, Hamilton, MT 59840, USA
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Laboratory of Bacteriology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, National Institute of Health, Hamilton, MT 59840, USA
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Faculty of Health: Medicine, Dentistry and Human Sciences, School of Biomedical Sciences, University of Plymouth, PL4 8AA, UK
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The Vaccine Group (TVG) Ltd., 14 Research Way, Derriford Research Facility, Plymouth Science Park, Plymouth PL6 8BU, UK
*
Authors to whom correspondence should be addressed.
Academic Editors: James Strong and David Safronetz
Viruses 2021, 13(5), 729; https://doi.org/10.3390/v13050729
Received: 22 March 2021 / Revised: 11 April 2021 / Accepted: 17 April 2021 / Published: 22 April 2021
(This article belongs to the Special Issue High-Consequence Viral Transmission)
The multimammate mouse (Mastomys natalensis; M. natalensis) has been identified as a major reservoir for multiple human pathogens including Lassa virus (LASV), Leishmania spp., Yersinia spp., and Borrelia spp. Although M. natalensis are related to well-characterized mouse and rat species commonly used in laboratory models, there is an absence of established assays and reagents to study the host immune responses of M. natalensis. As a result, there are major limitations to our understanding of immunopathology and mechanisms of immunological pathogen control in this increasingly important rodent species. In the current study, a large panel of commercially available rodent reagents were screened to identify their cross-reactivity with M. natalensis. Using these reagents, ex vivo assays were established and optimized to evaluate lymphocyte proliferation and cytokine production by M. natalensis lymphocytes. In contrast to C57BL/6J mice, lymphocytes from M. natalensis were relatively non-responsive to common stimuli such as phytohaemagglutinin P and lipopolysaccharide. However, they readily responded to concanavalin A stimulation as indicated by proliferation and cytokine production. In summary, we describe lymphoproliferative and cytokine assays demonstrating that the cellular immune responses in M. natalensis to commonly used mitogens differ from a laboratory-bred mouse strain. View Full-Text
Keywords: Mastomys natalensis; immune response; T cell; effector cytokines; concanavalin A; phytohaemagglutinin P; lipopolysaccharide; Lassa virus Mastomys natalensis; immune response; T cell; effector cytokines; concanavalin A; phytohaemagglutinin P; lipopolysaccharide; Lassa virus
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MDPI and ACS Style

Tang-Huau, T.-L.; Rosenke, K.; Meade-White, K.; Carmody, A.; Smith, B.J.; Bosio, C.M.; Jarvis, M.A.; Feldmann, H. Mastomys natalensis Has a Cellular Immune Response Profile Distinct from Laboratory Mice. Viruses 2021, 13, 729. https://doi.org/10.3390/v13050729

AMA Style

Tang-Huau T-L, Rosenke K, Meade-White K, Carmody A, Smith BJ, Bosio CM, Jarvis MA, Feldmann H. Mastomys natalensis Has a Cellular Immune Response Profile Distinct from Laboratory Mice. Viruses. 2021; 13(5):729. https://doi.org/10.3390/v13050729

Chicago/Turabian Style

Tang-Huau, Tsing-Lee, Kyle Rosenke, Kimberly Meade-White, Aaron Carmody, Brian J. Smith, Catharine M. Bosio, Michael A. Jarvis, and Heinz Feldmann. 2021. "Mastomys natalensis Has a Cellular Immune Response Profile Distinct from Laboratory Mice" Viruses 13, no. 5: 729. https://doi.org/10.3390/v13050729

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