Molecular Characterization of Imported and Autochthonous Dengue in Northeastern Spain
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Design
2.2. Nucleic Acid Extraction, DENV Genome Detection and DENV Serotyping
2.3. Genotyping of DENV and Phylogenetic Analysis
3. Results
3.1. Imported Dengue Cases
3.2. Molecular Characterization of Imported Dengue
3.3. First Autochthonous Case in Northeastern Spain
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primer | Sequence (5′-3′) | Localization | Ref. | ||||
---|---|---|---|---|---|---|---|
Envelope gene | DENV-1 | RT-PCR | F | EGENE1-S | CCGAAACGTGGATGTCCTCTGARGG | 756–780 | [24] |
R | EGENE-R | TCCTCCCATGCCTTCCCRATGG | 2553–2574 | ||||
Nested PCR | F | F Nested | ATAGGAACATCCATYACYCAG | 866–887 | Designed de novo | ||
R | EGENE/NS-RR | TGRAAYTTRTAYTGYTCTGTCC | 2502–2523 | [24] | |||
DENV-2 | RT-PCR | F | EGENE2-S | CTGAAACATGGATGTCATCAGAAGG | 758–782 | [24] | |
R | RRT 2 | GCYGARGCYARYTTTGARGGRG | 2533-2555 | Modified from [24] | |||
Nested PCR | F | F Nested | ATGGCRGCDATYYTGGCDYAY | 844–865 | Designed de novo | ||
R | R Nested | CGKGARTTCATYCCTATCCATGT | 2348–2371 | Designed de novo | |||
DENV-3 | RT-PCR | F | EGENE3-S | CTCAAACCTGGATGTCGGCTGARGG | 756–780 | [24] | |
R | RRT 3 | ATYCCRCAVACTCCATTYTYCC | 2561–2583 | Modified from [24] | |||
Nested PCR | F | F NESTED | ATGYTGGTCACYCCATCCATG | 911–932 | Designed de novo | ||
R | R NESTED | TTGTAYTGYTCTGTCCARGTRTG | 2511–2534 | Designed de novo | |||
DENV-4 | RT-PCR | F | EGENE4-S | CTGAGACATGGATGTCATCGGAAGG | 760–784 | [24] | |
R | RRT 4 | CACAGACCCCHTCTTTGTGRGC | 2567–2589 | Modified from [24] | |||
Nested PCR | F | F NESTED | TACTCAGRAABCCAGGATTYGC | 869–890 | Designed de novo | ||
R | R NESTED | YTCCATGACACYRCACAACCC | 2478–2470 | Designed de novo | |||
E/NS1 junction | DENV-1 | RT-PCR | F | FRTC 1 | TGSYTGAGACYCARCAYGGNAC | 1869–1890 | Modified from [23] |
R | RRTC 1 | YTCRTTTGATATYTGYYTCCAC | 2620–2641 | ||||
Nested PCR | F | FNC 1 | GRAAATGTTYGARGCHACYGCCC | 2130–2153 | Modified from [24] | ||
R | RNC 1 | TCYTCCCAYGCYYTYCCRATGG | 2553–2574 | ||||
DENV-2 | RT-PCR | F | FRTC 2 | TAGCWRRRACRCARCATGGAAC | 1871–1889 | Modified from [23] | |
R | RRTC 2 | CAGTTCYGGWGYTATYTGYYTCCAC | 2622–2646 | ||||
Nested PCR | F | FNC 2 | CARTYARYATAGAAGCAGARCC | 2027–2048 | Modified from [24] | ||
R | RNC 2 | GCYGAWGCYARYTTTGRRGGRG | 2534–2555 | ||||
DENV-3 | RT-PCR | F | FRTC 3 | TYTCHGARACRCARCAYGGRAC | 1863–1884 | Modified from [23] | |
R | RRTC 3 | BARYTCATTRGCTAYTTGCTTCCAY | 2614–2638 | ||||
Nested PCR | F | FNC 3 | GRRAARATGTTYGAGRCSMCYG | 2125–2146 | Modified from [24] | ||
R | RNC 3 | ATYCCRCAVACTCCATTYTYCC | 2562–2583 | ||||
DENV-4 | RT-PCR | F | FRTC 4 | TGGCAGAAACACARCATGGRAC | 1873–1894 | Modified from [23] | |
R | RRTC 4 | YARYTCRTTRGTTATTTGYTTCCAC | 2624–2648 | ||||
Nested PCR | F | FNC4 | ATYGGYAAGATGTTYGAGTCY | 2130–2150 | Modified from [24] | ||
R | RNC 4 | CACAGACCCCHTCTTTGTGRGC | 2568–2589 |
Serotype | Genotypes Detected | Number of Cases |
---|---|---|
DENV-1 | I | 15 |
IV | 3 | |
V | 16 | |
DENV-2 | Asian I | 3 |
American/Asian | 9 | |
Cosmopolitan | 21 | |
DENV-3 | I | 8 |
III | 10 | |
DENV-4 | I | 1 |
III | 4 |
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Navero-Castillejos, J.; Benitez, R.; Torner, N.; Muñoz, J.; Camprubí-Ferrer, D.; Peiró-Mestres, A.; Sulleiro, E.; Silgado, A.; Gonzalo, V.; Falgueras, T.; et al. Molecular Characterization of Imported and Autochthonous Dengue in Northeastern Spain. Viruses 2021, 13, 1910. https://doi.org/10.3390/v13101910
Navero-Castillejos J, Benitez R, Torner N, Muñoz J, Camprubí-Ferrer D, Peiró-Mestres A, Sulleiro E, Silgado A, Gonzalo V, Falgueras T, et al. Molecular Characterization of Imported and Autochthonous Dengue in Northeastern Spain. Viruses. 2021; 13(10):1910. https://doi.org/10.3390/v13101910
Chicago/Turabian StyleNavero-Castillejos, Jessica, Rosa Benitez, Nuria Torner, José Muñoz, Daniel Camprubí-Ferrer, Aida Peiró-Mestres, Elena Sulleiro, Aroa Silgado, Verónica Gonzalo, Teresa Falgueras, and et al. 2021. "Molecular Characterization of Imported and Autochthonous Dengue in Northeastern Spain" Viruses 13, no. 10: 1910. https://doi.org/10.3390/v13101910
APA StyleNavero-Castillejos, J., Benitez, R., Torner, N., Muñoz, J., Camprubí-Ferrer, D., Peiró-Mestres, A., Sulleiro, E., Silgado, A., Gonzalo, V., Falgueras, T., Alejo-Cancho, I., Roldán, M., Plasencia, V., Albarracin, R., Perez, J., Navarro, A., Calderón, A., Rubio, R., Navarro, M., ... Martínez, M. J. (2021). Molecular Characterization of Imported and Autochthonous Dengue in Northeastern Spain. Viruses, 13(10), 1910. https://doi.org/10.3390/v13101910