Inactivation of Human Coronavirus by Titania Nanoparticle Coatings and UVC Radiation: Throwing Light on SARS-CoV-2
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells
2.2. Human Coronavirus
2.3. TiO2 Nanoparticles (TNPs)-Coated Glass Coverslips
2.4. UV Photocatalysis
2.5. Virus Infectivity and Immunofluorescence Assay (IFA)
2.6. RNA Extraction and qPCR
2.7. Experimental Setup
2.8. Statistical Analysis
3. Results
3.1. The Effect of UV Light Exposure on HCoV-NL63 Virus Stability
3.2. TNP Coating Efficiently Inactivated HCoV-NL63 Even at Brief Exposures
3.3. TiO2 Coatings Effectively Inactivated HCoV-NL63 Virus Present in Wet or Dried Form
3.4. Reducing the Exposure Distance and Increasing the Relative Humidity Enhanced HCoV-NL63 Inactivation
4. Discussion
5. Patents
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Khaiboullina, S.; Uppal, T.; Dhabarde, N.; Subramanian, V.R.; Verma, S.C. Inactivation of Human Coronavirus by Titania Nanoparticle Coatings and UVC Radiation: Throwing Light on SARS-CoV-2. Viruses 2021, 13, 19. https://doi.org/10.3390/v13010019
Khaiboullina S, Uppal T, Dhabarde N, Subramanian VR, Verma SC. Inactivation of Human Coronavirus by Titania Nanoparticle Coatings and UVC Radiation: Throwing Light on SARS-CoV-2. Viruses. 2021; 13(1):19. https://doi.org/10.3390/v13010019
Chicago/Turabian StyleKhaiboullina, Svetlana, Timsy Uppal, Nikhil Dhabarde, Vaidyanathan Ravi Subramanian, and Subhash C. Verma. 2021. "Inactivation of Human Coronavirus by Titania Nanoparticle Coatings and UVC Radiation: Throwing Light on SARS-CoV-2" Viruses 13, no. 1: 19. https://doi.org/10.3390/v13010019