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Article

Next-Generation Sequencing-Based Quantitative Detection of Hepatitis B Virus Pre-S Mutants in Plasma Predicts Hepatocellular Carcinoma Recurrence

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Graduate Institute of Biomedical Sciences, China Medical University, No.91, Hsueh-Shih Rd., Northern Dist., Taichung City 404, Taiwan
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Organ Transplantation Center, China Medical University Hospital, No.2, Yude Rd., Northern Dist., Taichung City 404, Taiwan
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Research Center for Cancer Biology, China Medical University, Taichung City 404, Taiwan
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Department of Public Health, College of Public Health, China Medical University, Taichung City 404, Taiwan
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Department of Healthcare Administration, College of Medical and Health Science, Asia University, Taichung City 413, Taiwan
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Department of Laboratory Medicine, China Medical University Hospital, Taichung City 404, Taiwan
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Department of Occupational Therapy, Asia University, Taichung City 413, Taiwan
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Department of Neurology, China Medical University Hospital, Taichung City 404, Taiwan
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Translational Medicine Research Center, China Medical University Hospital, Taichung City 404, Taiwan
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National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan 350, Taiwan
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Division of Hepatogastroenterology, Department of Internal Medicine, China Medical University Hospital, Taichung City 404, Taiwan
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School of Medicine, China Medical University, Taichung City 404, Taiwan
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Department of Biotechnology, Southern Taiwan University of Science and Technology, No.1, Nantai St., Yongkang Dist., Tainan City 710, Taiwan
*
Authors to whom correspondence should be addressed.
Viruses 2020, 12(8), 796; https://doi.org/10.3390/v12080796
Received: 12 June 2020 / Revised: 9 July 2020 / Accepted: 23 July 2020 / Published: 24 July 2020
(This article belongs to the Special Issue Viral Molecular Epidemiology)
Hepatocellular carcinoma (HCC) is among the most common and lethal human cancers worldwide. Despite curative resection, high recurrence of HCC remains a big threat, leading to poor patient outcomes. Hepatitis B virus (HBV) pre-S mutants, which harbor deletions over pre-S1 and pre-S2 gene segments of large surface proteins, have been implicated in HCC recurrence. Therefore, a reliable approach for detection of pre-S mutants is urgently needed for predicting HCC recurrence to improve patient survival. In this study, we used a next-generation sequencing (NGS)-based platform for quantitative detection of pre-S mutants in the plasma of HBV-related HCC patients and evaluated their prognostic values in HCC recurrence. We demonstrated that the presence of deletions spanning the pre-S2 gene segment and the high percentage of pre-S2 plus pre-S1 + pre-S2 deletions, either alone or in combination, was significantly and independently associated with poor recurrence-free survival and had greater prognostic performance than other clinicopathological and viral factors in predicting HCC recurrence. Our data suggest that the NGS-based quantitative detection of pre-S mutants in plasma represents a promising approach for identifying patients at high risk for HBV-related HCC recurrence after surgical resection in a noninvasive manner. View Full-Text
Keywords: hepatocellular carcinoma; hepatitis B virus; pre-S mutants; recurrence; next-generation sequencing hepatocellular carcinoma; hepatitis B virus; pre-S mutants; recurrence; next-generation sequencing
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MDPI and ACS Style

Teng, C.-F.; Li, T.-C.; Huang, H.-Y.; Lin, J.-H.; Chen, W.-S.; Shyu, W.-C.; Wu, H.-C.; Peng, C.-Y.; Su, I.-J.; Jeng, L.-B. Next-Generation Sequencing-Based Quantitative Detection of Hepatitis B Virus Pre-S Mutants in Plasma Predicts Hepatocellular Carcinoma Recurrence. Viruses 2020, 12, 796. https://doi.org/10.3390/v12080796

AMA Style

Teng C-F, Li T-C, Huang H-Y, Lin J-H, Chen W-S, Shyu W-C, Wu H-C, Peng C-Y, Su I-J, Jeng L-B. Next-Generation Sequencing-Based Quantitative Detection of Hepatitis B Virus Pre-S Mutants in Plasma Predicts Hepatocellular Carcinoma Recurrence. Viruses. 2020; 12(8):796. https://doi.org/10.3390/v12080796

Chicago/Turabian Style

Teng, Chiao-Fang; Li, Tsai-Chung; Huang, Hsi-Yuan; Lin, Jia-Hui; Chen, Wen-Shu; Shyu, Woei-Cherng; Wu, Han-Chieh; Peng, Cheng-Yuan; Su, Ih-Jen; Jeng, Long-Bin. 2020. "Next-Generation Sequencing-Based Quantitative Detection of Hepatitis B Virus Pre-S Mutants in Plasma Predicts Hepatocellular Carcinoma Recurrence" Viruses 12, no. 8: 796. https://doi.org/10.3390/v12080796

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