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Viruses of Microbes
Open AccessArticle

A Lactococcal Phage Protein Promotes Viral Propagation and Alters the Host Proteomic Response During Infection

1
Département de Biochimie, de Microbiologie, et de Bio-Informatique, Faculté des Sciences et de Génie and PROTEO, Québec City, QC G1V 0A6, Canada
2
Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, Québec City, QC G1V 0A6, Canada
3
Institute of Microbiology, University of Greifswald, 17489 Greifswald, Germany
4
Institut de Biologie Intégrative et des Systèmes, Université Laval, Québec City, QC G1V 0A6, Canada
5
Centre de Recherche en Infectiologie de L’Université Laval, Axe Maladies Infectieuses et Immunitaires, Centre de Recherche du CHU de Québec-Université Laval, Québec City, QC G1V 4G2, Canada
6
Centre de Recherche en Données Massives, Université Laval, Québec City, QC G1V 0A6, Canada
7
Félix D’Hérelle Reference Center for Bacterial Viruses, Université Laval, Québec City, QC G1V 0A6, Canada
8
Département de Médecine Moléculaire, Faculté de Médecine, Université Laval, Québec City, QC G1V 0A6, Canada
*
Author to whom correspondence should be addressed.
Present address: Université de Montréal, Montreal, QC H3T 1J4, Canada.
Present address: Agilent Technologies, Waldbronn, 76337 Baden-Württemberg, Germany.
Viruses 2020, 12(8), 797; https://doi.org/10.3390/v12080797
Received: 17 June 2020 / Revised: 21 July 2020 / Accepted: 22 July 2020 / Published: 24 July 2020
(This article belongs to the Special Issue Viruses of Microbes 2020: The Latest Conquests on Viruses of Microbes)
The lactococcal virulent phage p2 is a model for studying the Skunavirus genus, the most prevalent group of phages causing milk fermentation failures in cheese factories worldwide. This siphophage infects Lactococcus lactis MG1363, a model strain used to study Gram-positive lactic acid bacteria. The structural proteins of phage p2 have been thoroughly described, while most of its non-structural proteins remain uncharacterized. Here, we developed an integrative approach, making use of structural biology, genomics, physiology, and proteomics to provide insights into the function of ORF47, the most conserved non-structural protein of unknown function among the Skunavirus genus. This small phage protein, which is composed of three α-helices, was found to have a major impact on the bacterial proteome during phage infection and to significantly reduce the emergence of bacteriophage-insensitive mutants. View Full-Text
Keywords: phages; Skunavirus; Lactococcus lactis; non-structural phage proteins; phage-host interactions; bacterial proteomes; phage-resistant mutants phages; Skunavirus; Lactococcus lactis; non-structural phage proteins; phage-host interactions; bacterial proteomes; phage-resistant mutants
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MDPI and ACS Style

Lemay, M.-L.; Maaß, S.; Otto, A.; Hamel, J.; Plante, P.-L.; Rousseau, G.M.; Tremblay, D.M.; Shi, R.; Corbeil, J.; Gagné, S.M.; Becher, D.; Moineau, S. A Lactococcal Phage Protein Promotes Viral Propagation and Alters the Host Proteomic Response During Infection. Viruses 2020, 12, 797. https://doi.org/10.3390/v12080797

AMA Style

Lemay M-L, Maaß S, Otto A, Hamel J, Plante P-L, Rousseau GM, Tremblay DM, Shi R, Corbeil J, Gagné SM, Becher D, Moineau S. A Lactococcal Phage Protein Promotes Viral Propagation and Alters the Host Proteomic Response During Infection. Viruses. 2020; 12(8):797. https://doi.org/10.3390/v12080797

Chicago/Turabian Style

Lemay, Marie-Laurence; Maaß, Sandra; Otto, Andreas; Hamel, Jérémie; Plante, Pier-Luc; Rousseau, Geneviève M.; Tremblay, Denise M.; Shi, Rong; Corbeil, Jacques; Gagné, Stéphane M.; Becher, Dörte; Moineau, Sylvain. 2020. "A Lactococcal Phage Protein Promotes Viral Propagation and Alters the Host Proteomic Response During Infection" Viruses 12, no. 8: 797. https://doi.org/10.3390/v12080797

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