Protein Arginine N-methyltransferases 5 and 7 Promote HIV-1 Production
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Culture Conditions
2.2. Expression Vectors
2.3. Identification of Vpr-Binding Proteins
2.4. Glutathione-S-Transferase (GST) Pull-Down Assay
2.5. Immunoblotting
2.6. Co-Immunoprecipitation
2.7. siRNA and Transfection
2.8. Quantitative PCR (qPCR)
2.9. Viruses and Infection
2.10. Statistical Analysis
3. Results
3.1. Identification of Vpr-Binding Protein Derived from Human MDMs
3.2. Four PRMT Family Proteins Bind to Vpr
3.3. Vpr Expression Affected by PRMT5 and PRMT7
3.4. Inhibition of Proteasome-Dependent Vpr Degradation by Two Vpr-Binding PRMTs
3.5. Knockdown of Vpr-Binding PRMTs in MDMs Inhibited Efficient Virus Production
3.6. Low Expression of PRMT5 Affected Wild-Type HIV-1 Replication in MDMs
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Murakami, H.; Suzuki, T.; Tsuchiya, K.; Gatanaga, H.; Taura, M.; Kudo, E.; Okada, S.; Takei, M.; Kuroda, K.; Yamamoto, T.; et al. Protein Arginine N-methyltransferases 5 and 7 Promote HIV-1 Production. Viruses 2020, 12, 355. https://doi.org/10.3390/v12030355
Murakami H, Suzuki T, Tsuchiya K, Gatanaga H, Taura M, Kudo E, Okada S, Takei M, Kuroda K, Yamamoto T, et al. Protein Arginine N-methyltransferases 5 and 7 Promote HIV-1 Production. Viruses. 2020; 12(3):355. https://doi.org/10.3390/v12030355
Chicago/Turabian StyleMurakami, Hironobu, Takehiro Suzuki, Kiyoto Tsuchiya, Hiroyuki Gatanaga, Manabu Taura, Eriko Kudo, Seiji Okada, Masami Takei, Kazumichi Kuroda, Tatsuo Yamamoto, and et al. 2020. "Protein Arginine N-methyltransferases 5 and 7 Promote HIV-1 Production" Viruses 12, no. 3: 355. https://doi.org/10.3390/v12030355