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Article

Maturation of Pseudo-Nucleus Compartment in P. aeruginosa, Infected with Giant phiKZ Phage

1
Biology Department, Moscow Lomonosov University, 119234 Moscow, Russia
2
Research Center of Nanobiotechnologies, Peter the Great St. Petersburg Polytechnic University, 195251 St. Petersburg, Russia
3
Semenov Institute of Chemical Physics, Russian Academy of Sciences, 117977 Moscow, Russia
4
Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg, Russia
5
Laboratory of Evolution of Sensory Organs, Sechenov Institute of Evolutionary Physiology and Biochemistry Russian Academy of Sciences, 194223 St. Petersburg, Russia
6
Biology Department, Shenzhen MSU-BIT University, Shenzhen 518172, China
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(10), 1197; https://doi.org/10.3390/v12101197
Received: 26 August 2020 / Revised: 19 October 2020 / Accepted: 19 October 2020 / Published: 21 October 2020
(This article belongs to the Special Issue Giant or Jumbo Phages)
The giant phiKZ phage infection induces the appearance of a pseudo-nucleus inside the bacterial cytoplasm. Here, we used RT-PCR, fluorescent in situ hybridization (FISH), electron tomography, and analytical electron microscopy to study the morphology of this unique nucleus-like shell and to demonstrate the distribution of phiKZ and bacterial DNA in infected Pseudomonas aeruginosa cells. The maturation of the pseudo-nucleus was traced in short intervals for 40 min after infection and revealed the continuous spatial separation of the phage and host DNA. Immediately after ejection, phage DNA was located inside the newly-identified round compartments; at a later infection stage, it was replicated inside the pseudo-nucleus; in the mature pseudo-nucleus, a saturated internal network of filaments was observed. This network consisted of DNA bundles in complex with DNA-binding proteins. On the other hand, the bacterial nucleoid underwent significant rearrangements during phage infection, yet the host DNA did not completely degrade until at least 40 min after phage application. Energy dispersive x-ray spectroscopy (EDX) analysis revealed that, during the infection, the sulfur content in the bacterial cytoplasm increased, which suggests an increase of methionine-rich DNA-binding protein synthesis, whose role is to protect the bacterial DNA from stress caused by infection. View Full-Text
Keywords: giant phage; phiKZ; Pseudomonas aeruginosa; nucleoid; pseudo-nucleus; analytical electron microscopy; electron tomography; fluorescent in situ hybridization; stress response giant phage; phiKZ; Pseudomonas aeruginosa; nucleoid; pseudo-nucleus; analytical electron microscopy; electron tomography; fluorescent in situ hybridization; stress response
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MDPI and ACS Style

Danilova, Y.A.; Belousova, V.V.; Moiseenko, A.V.; Vishnyakov, I.E.; Yakunina, M.V.; Sokolova, O.S. Maturation of Pseudo-Nucleus Compartment in P. aeruginosa, Infected with Giant phiKZ Phage. Viruses 2020, 12, 1197. https://doi.org/10.3390/v12101197

AMA Style

Danilova YA, Belousova VV, Moiseenko AV, Vishnyakov IE, Yakunina MV, Sokolova OS. Maturation of Pseudo-Nucleus Compartment in P. aeruginosa, Infected with Giant phiKZ Phage. Viruses. 2020; 12(10):1197. https://doi.org/10.3390/v12101197

Chicago/Turabian Style

Danilova, Yana A.; Belousova, Viktoriia V.; Moiseenko, Andrey V.; Vishnyakov, Innokentii E.; Yakunina, Maria V.; Sokolova, Olga S. 2020. "Maturation of Pseudo-Nucleus Compartment in P. aeruginosa, Infected with Giant phiKZ Phage" Viruses 12, no. 10: 1197. https://doi.org/10.3390/v12101197

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